Abstract
1 Norfenfluramine is thought to be the major active metabolite of fenfluramine in man. Therefore its in vitro effect on lipogenesis in human adipose tissue from twelve patients was investigated.
2 Although inhibition of lipogenesis was demonstrated, the concentrations of norfenfluramine necessary (>1 mM) were much higher than normal blood levels of the drug.
3 There was no correlation between the degree of inhibition by norfenfluramine in vitro and fat cell size, age or relative weight of the subject. Greater degrees of inhibition were however associated with samples of tissue from patients with higher blood glycerol concentrations and lower blood triglyceride concentrations.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Campbell D. B. Gas chromatographic measurement of levels of fenfluramine and norfenfluramine in human plasma, red cells and urine following therapeutic doses. J Chromatogr. 1970 Jun 24;49(3):442–447. doi: 10.1016/s0021-9673(00)93657-4. [DOI] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- Garrow J. S., Belton E. A., Daniels A. A controlled investigation of the "glycolyptic" action of fenfluramine. Lancet. 1972 Sep 16;2(7777):559–561. doi: 10.1016/s0140-6736(72)91957-5. [DOI] [PubMed] [Google Scholar]
- Gilbert C. H., Kaye J., Galton D. J. The effect of a glucose load on plasma fatty acids and lipolysis in adipose tissue of obese diabetic and non-diabetic patients. Diabetologia. 1974 Apr;10(2):135–138. doi: 10.1007/BF01219669. [DOI] [PubMed] [Google Scholar]
- Pawan G. L. Effect of fenfluramine on blood-lipids in man. Lancet. 1969 Mar 8;1(7593):498–500. doi: 10.1016/s0140-6736(69)91594-3. [DOI] [PubMed] [Google Scholar]
- RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
- Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol. 1969 Mar;22(2):158–161. doi: 10.1136/jcp.22.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson J. P., Galton D. J. The effect of drugs on lipogenesis from glucose and palmitate in human adipose tissue. Horm Metab Res. 1971 Jul;3(4):262–266. doi: 10.1055/s-0028-1094145. [DOI] [PubMed] [Google Scholar]