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Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2010 Feb 10;25(1):82–85. doi: 10.1007/s12291-010-0017-y

Role of reactive oxygen species in pathogenesis of nephrotic syndrome

Santoshi R Ghodake 1,✉, A N Suryakar 2, R D Ankush 2, K Shaikh 1, A V Katta 1
PMCID: PMC3453014  PMID: 23105890

Abstract

Nephrotic syndrome is the common chronic disorder characterized by alteration of permeability of the glomerular capillary wall, resulting in its inability to restrict the urinary loss of proteins. Nephrotic syndrome is characterized by heavy proteinuria, hypoalbuminemia, hyperlipidemia associated with peripheral edema. The molecular basis of glomerular permselectivity remains largely unknown. In recent years it has been proposed that Nephrotic syndrome is a consequence of an imbalance between oxidant and antioxidant activity. The present study was aimed to test that the reactive oxygen species are the mediators of excessive protein permeability and other complications of Nephrotic syndrome. For this 30 adults with Nephrotic syndrome were studied. The control group comprised 30 healthy adults matched for age. Serum levels of lipid peroxides, nitric oxide (NO⊙), α- tocopherol, ascorbic acid, erythrocyte superoxide dismutase activity, serum albumin, uric acid, cholesterol and plasma total antioxidant capacity were measured. Student’s ‘t’ test was applied for statistical analysis. There was a significant increase in lipid peroxide (1.58 ± 0.42 in controls, 3.64 ±1.3 in patients) (P<0.001) levels in study group as compared with controls. α-tocopherol (12.95 ± 1.04 in controls, 9.93 ± 1.43 in patients) (P<0.001), erythrocyte SOD activity(1.88 ± 0.9 in controls 1.07 ± 0.5 in patients) (P=0.01), serum albumin(4.06 ± 0.50 in controls, 3.04 ± 0.11 in patients) (P<0.001), and plasma total antioxidant capacity (847.33 ± 126.83 in controls, 684.00±102.94 in patients) (P<0.001) were significantly decreased. There was non-significant increase in uric acid (P>0.05), a non-significant decrease in NO⊙ (38.48 ± 15.47 in controls 37.47 ± 14.27 in patients) (P>0.05) and ascorbic acid levels ascorbic acid,( 0.95 ± 0.31in controls 0.79 ± 0.30 in patients) (P>0.05) in study group as compared with controls. Imbalance between oxidants and antioxidants may contribute to pathogenesis of proteinuria and related complications in nephrotic syndrome.

Key Words: Nephrotic syndrome, Lipid peroxide, Antioxidants, Erythrocyte Superoxide dismutase

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References

  • 1.Zachwieja J., Bobkowski W., Zaniew M., Dobrowolska-Zachwieja A., Lewandowska-Stachowiak M., Siwinska A. Apoptosis and antioxidant defense in the nephrotic syndrome. Pediatr Nephrol. 2003;18:1116–1121. doi: 10.1007/s00467-003-1250-x. [DOI] [PubMed] [Google Scholar]
  • 2.McBryde K.D., Kershaw D.B., Smoyer W.E. Pediatric steroid-resistant nephrotic syndrome. Curr Probl Pediatr. 2001;31:275–307. doi: 10.1067/mps.2001.119800. [DOI] [PubMed] [Google Scholar]
  • 3.Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chemical Acta. 1978;90:37–43. doi: 10.1016/0009-8981(78)90081-5. [DOI] [PubMed] [Google Scholar]
  • 4.Cortas N.K., Wakid N.W. Determination of inorganic nitrate in serum and urine by kinetic cadmium — reduction method. Clin Chem. 1990;36(8):1440–1143. [PubMed] [Google Scholar]
  • 5.Doumas (Modified Spencer and Price 1977). Determination of serum albumin using Bromocresol Green. Practical Clinical Biochemistry; Heinemann professional publishing 5th edition; 1980: 553–554.
  • 6.Das K. A modified spectrophotometric assay of superoxide dismutase using nitrate formation by superoxide radicals. Ind J Biochem Biophys. 2000;57:201–204. [PubMed] [Google Scholar]
  • 7.Baker, Frank. Determination of Serum tocopherol by colorimetric method. In Varley’s practical Clinical Biochemistry, 6th edition: Heinmann Professional Publishing 1988; 902.
  • 8.Caraway WT. Carbohydrates in Tietz NW (Ed): Fundamentals of clinical chemistry. W B Saunder Company 173–176.
  • 9.Iris F.F., Benzie, Strain J.J. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochem. 1996;239(1):70–76. doi: 10.1006/abio.1996.0292. [DOI] [PubMed] [Google Scholar]
  • 10.Dogra G., Ward N., Croft K.D., Mori T.A., Barrett P.H.R., Herrmann S.E., et al. Oxidant stress in nephrotic syndrome: comparison of F2-isoprostanes and plasma antioxidant potential. Nephrol Dial Transplant. 2001;16:1626–1630. doi: 10.1093/ndt/16.8.1626. [DOI] [PubMed] [Google Scholar]
  • 11.Kinra S., Rath B., Kabi B.C. Indirect quantification of lipid per oxidation in steroid responsive nephrotic syndrome. Arch Dist Child. 2000;82:76–78. doi: 10.1136/adc.82.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Raasts C.J.I., Bakker M.A.H., Born J.V.D., Berden J.H.M. Hydroxyl radicals depolymerize glomerular heparan sulphates in vitro and in experimental nephrotic syndrome. J Biol Chem. 1997;272(42):26734–36741. doi: 10.1074/jbc.272.42.26734. [DOI] [PubMed] [Google Scholar]
  • 13.Raats C.J.I., Born J.V.D., Berden J.H.M. Glomerular heparin sulfates alterations: Mechanism and relevance for proteinuria. Kidney Int. 2000;57:385–400. doi: 10.1046/j.1523-1755.2000.00858.x. [DOI] [PubMed] [Google Scholar]
  • 14.Isaacs B.R., Pawar B., Sajiv C.T., Calton N., Isaacs R. Barraquer — Simons Disease-A Rare cause of proteinuria. Ind J Nephrol. 1997;7(1):31–32. [Google Scholar]
  • 15.Bryde K.D.M., Kershaw D.B., Smoyer W.E. Pediatric steroid-resistant nephrotic syndrome. Curr Probl Pediatr. 2001;31:275–307. doi: 10.1067/mps.2001.119800. [DOI] [PubMed] [Google Scholar]
  • 16.Wever R., Boer P., Hampering M., Stores E., Gerhard M., Versluis J.K.K., et al. Nitric oxide production is reduced in patients with chronic renal failure. Arterioscler Thromb Vasc Biol. 1999;19:1168–1172. doi: 10.1161/01.atv.19.5.1168. [DOI] [PubMed] [Google Scholar]
  • 17.Ni Z., Vaziri N.D. Downregulation of nitric oxide synthase in nephrotic syndrome: role of proteinuria. Biochim Biophys Acta. 2003;1638(2):129–137. doi: 10.1016/s0925-4439(03)00061-9. [DOI] [PubMed] [Google Scholar]
  • 18.Duran P., Datta P.K., Pan C., Blumberg J.B., Sharma M., Lianos E.A. Superoxide dismutase mimetic preserves the glomerular capillary permeability barrier to protein. J Pharmacol Experimental Therapeutics. 2006;316(3):1249–1254. doi: 10.1124/jpet.105.092957. [DOI] [PubMed] [Google Scholar]
  • 19.Goldstone A., Rastegar R., Shariftabrizi A., Payabvash S.K.S.M., Salmasi A.H., Dehpour A.R., et al. Paradoxical dose and time- dependent regulation of superoxide dismutase and antioxidant capacity by vitamin E in rat. Clin Chim Acta. 2006;265:153–159. doi: 10.1016/j.cca.2005.08.008. [DOI] [PubMed] [Google Scholar]
  • 20.Ichikawa I., Kiyama S., Yoshioka T. Renal antioxidant enzymes: Their regulation and function. Kidney Int. 1994;45:1–9. doi: 10.1038/ki.1994.1. [DOI] [PubMed] [Google Scholar]
  • 21.Tian J., Chen J.H., Li Q., He Q., Lin W.Q. Lipid peroxidation in IgA nephropathy and the effect of lipo — prostaglandin E1. J Nephrol. 2005;18:243–248. [PubMed] [Google Scholar]
  • 22.Young I.S., Woodside J.V. Antioxidants in health and disease. J. Clin Petrol. 2001;54:176–186. doi: 10.1136/jcp.54.3.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Padayatti S.J., Katz A., Wang Y., Eck P., Kwon O., Lee J.H., et al. Vitamin C as an antioxidant: Evaluation of its role in disease prevention. J Am College of Nutr. 2003;22(1):18–35. doi: 10.1080/07315724.2003.10719272. [DOI] [PubMed] [Google Scholar]
  • 24.Rajbala A., Sane A.S., Cope J., Mishra V.V., Trivedi H.L. Oxidative stress status in children with nephrotic syndrome. Panminerva Med. 1997;39(3):165–168. [PubMed] [Google Scholar]
  • 25.Ames B.N., Cathcart R., Schwiers E., Hochstein P. Uric acid provides an antioxidant defense in human aging and cancer: A hypothesis. Proc Natl Acad Sci USA. 1981;78(11):6858–6862. doi: 10.1073/pnas.78.11.6858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Bourdon E., Loreau N., Blache D. Glucose and free radicals impair the antioxidant properties of serum albumin. FASEB J. 1999;13:233–244. doi: 10.1096/fasebj.13.2.233. [DOI] [PubMed] [Google Scholar]
  • 27.Zachwieja J., Bobkdwski Dobrowolska-Zachwieja A., Zaniew M., Maciejewsk J. Decreased antioxidant activity in hypercholesterolemic children with nephrotic syndrome. Med Sci Monit. 2003;9(6):CR 287–CR 291. [PubMed] [Google Scholar]
  • 28.Vaziri N.D., Liang K., Parks J.S. Acquired lecithin — cholesterol acyltransferase deficiency in nephrotic syndrome. Am J Physiol Renal Physiol. 2001;49:F 823–F 828. doi: 10.1152/ajprenal.2001.280.5.F823. [DOI] [PubMed] [Google Scholar]

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