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. 1990 Mar;66(773):195–199. doi: 10.1136/pgmj.66.773.195

Endometrial oxytocin binding sites in normal women and in subfertile patients.

P N Baker 1, M L Peat 1, E M Symonds 1, P V Maynard 1
PMCID: PMC2429461  PMID: 2362885

Abstract

Specific binding of oxytocin to high affinity sites in endometrial membrane preparations has previously been shown in sheep. Endometrial tissue preparations from 27 'normal' women of proven fertility were incubated with tritiated oxytocin and the existence of significant binding sites in human endometrium was shown. Furthermore, the level of binding sites underwent a cyclical variation with the highest concentration of binding at midcycle. A cyclical pattern of binding site concentration not unlike that found in the normal women was shown in 19 subfertile patients taking clomiphene. However, in 20 subfertile patients not taking clomiphene, no cyclical pattern emerged with significantly lower levels of binding sites in the mid-portion of the cycle (P less than 0.02) and significantly higher levels in the mid-late luteal phase (P less than 0.01), as compared to the normal women. In the mid-portion of the cycle levels were significantly lower in the subfertile patients not taking clomiphene (P less than 0.03) as compared to those taking clomiphene. No significant differences were shown between the normal women and those patients taking clomiphene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Drake T. S., Tredway D. R., Buchanan G. C. Continued clinical experience with an increasing dosage regimen of clomiphene citrate administration. Fertil Steril. 1978 Sep;30(3):274–277. [PubMed] [Google Scholar]
  2. Fairclough R. J., Moore L. G., McGowan L. T., Peterson A. J., Smith J. F., Tervit H. R., Watkins W. B. Temporal relationship between plasma concentrations of 13,14-dihydro-15-keto-prostaglandin F and neurophysin I/II around luteolysis in sheep. Prostaglandins. 1980 Aug;20(2):199–208. doi: 10.1016/s0090-6980(80)80039-6. [DOI] [PubMed] [Google Scholar]
  3. Fleming R., Coutts J. R. Effects of clomiphene treatment on infertile women with normal menstrual rhythm. Br J Obstet Gynaecol. 1982 Sep;89(9):749–753. doi: 10.1111/j.1471-0528.1982.tb05103.x. [DOI] [PubMed] [Google Scholar]
  4. Gautray J. P., Mortel R., Robel P., Baulieu E. E. Uterine receptors and fertility regulation. Acta Eur Fertil. 1980 Sep;11(3):185–198. [PubMed] [Google Scholar]
  5. Isotalo H., Isomaa V., Jänne O. Replenishment and properties of cytosol progesterone receptor in rabbit uterus after multiple progesterone administrations. Endocrinology. 1981 Mar;108(3):868–873. doi: 10.1210/endo-108-3-868. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Leavitt W. W., Chen T. J., Allen T. C. Regulation of progesterone receptor formation by estrogen action. Ann N Y Acad Sci. 1977 Mar 11;286:210–225. doi: 10.1111/j.1749-6632.1977.tb29418.x. [DOI] [PubMed] [Google Scholar]
  8. Levy C., Robel P., Gautray J. P., De Brux J., Verma U., Descomps B., Baulieu E. E. Estradiol and progesterone receptors in human endometrium: normal and abnormal menstrual cycles and early pregnancy. Am J Obstet Gynecol. 1980 Mar 1;136(5):646–651. doi: 10.1016/0002-9378(80)91018-2. [DOI] [PubMed] [Google Scholar]
  9. Maynard P. V., Baker P. N., Symonds E. M., Sant-Cassia L. J., Johnson J., Selby C. Nuclear progesterone uptake by endometrial tissue in cases of subfertility. Lancet. 1983 Aug 6;2(8345):310–312. doi: 10.1016/s0140-6736(83)90291-x. [DOI] [PubMed] [Google Scholar]
  10. Nissenson R., Fluoret G., Hechter O. Opposing effects of estradiol and progesterone on oxytocin receptors in rabbit uterus. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2044–2048. doi: 10.1073/pnas.75.4.2044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pliska V., Heiniger J., Müller-Lhotsky A., Pliska P., Ekberg B. Binding of oxytocin to uterine cells in vitro. Occurrence of several binding site populations and reidentification of oxytocin receptors. J Biol Chem. 1986 Dec 25;261(36):16984–16989. [PubMed] [Google Scholar]
  12. Roberts J. S., Barcikowski B., Wilson L., Skarnes R. C., McCracken J. A. Hormonal and related factors affecting the release of prostaglandin F2alpha from the uterus. J Steroid Biochem. 1975 Jun;6(6):1091–1097. doi: 10.1016/0022-4731(75)90354-4. [DOI] [PubMed] [Google Scholar]
  13. Roberts J. S., McCracken J. A., Gavagan J. E., Soloff M. S. Oxytocin-stimulated release of prostaglandin F2alpha from ovine endometrium in vitro: correlation with estrous cycle and oxytocin-receptor binding. Endocrinology. 1976 Oct;99(4):1107–1114. doi: 10.1210/endo-99-4-1107. [DOI] [PubMed] [Google Scholar]
  14. Sharma S. C., Fitzpatrick R. J. Effect of oestradiol-17beta and oxytocin treatment on prostaglandin F alpha release in the anoestrous ewe. Prostaglandins. 1974 Apr 25;6(2):97–105. doi: 10.1016/0090-6980(74)90021-5. [DOI] [PubMed] [Google Scholar]
  15. Sheldrick E. L., Flint A. P. Endocrine control of uterine oxytocin receptors in the ewe. J Endocrinol. 1985 Aug;106(2):249–258. doi: 10.1677/joe.0.1060249. [DOI] [PubMed] [Google Scholar]
  16. Soloff M. S., Rees H. D., Sar M., Stumpf W. E. Autoradiographic localization of radioactivity from [3-H]oxytocin in the rat mammary gland and oviduct. Endocrinology. 1975 Jun;96(6):1475–1477. doi: 10.1210/endo-96-6-1475. [DOI] [PubMed] [Google Scholar]
  17. Templeton A. A., Penney G. C. The incidence, characteristics, and prognosis of patients whose infertility is unexplained. Fertil Steril. 1982 Feb;37(2):175–182. doi: 10.1016/s0015-0282(16)46035-8. [DOI] [PubMed] [Google Scholar]

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