Abstract
Anabaena cylindrica sparged with argon gas produced H2 continuously for 30 days under limited light conditions (6.0 W/m2) and for 18 days under elevated light conditions (32 W/m2) in the absence of exogenous nitrogen. The efficiency of converting visible light energy (32 W/m2) into chemical energy that is trapped as H2 ranged between 0.35 and 0.85% (approximately 13 microliter of H2 per mg [drywt] per h). Ammonium additions (0.2 mM NH4+) at various times destabilized the system and eventually suppressed H2 production completely, as compared with the control. Cultures grown with 5.0 mg of Fe3+ per liter produced H2 at a rate about twice that of cultures with 0.5 mg of Fe3+ per liter. Cultures grown at pH 7.4 produced H2 at the same initial rates as cultures that were grown at pH 9.4; however, the latter cultures continued to produce H2 after CO2 deprivation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen M. M., Stanier R. Y. Selective isolation of blue-green algae from water and soil. J Gen Microbiol. 1968 Apr;51(2):203–209. doi: 10.1099/00221287-51-2-203. [DOI] [PubMed] [Google Scholar]
- Benemann J. R., Berenson J. A., Kaplan N. O., Kamen M. D. Hydrogen evolution by a chloroplast-ferredoxin-hydrogenase system. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2317–2320. doi: 10.1073/pnas.70.8.2317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bothe H., Tennigkeit J., Eisbrenner G. The utilization of molecular hydrogen by the blue-green alga Anabaena cylindrica. Arch Microbiol. 1977 Jul 26;114(1):43–49. doi: 10.1007/BF00429628. [DOI] [PubMed] [Google Scholar]
- Donze M., Haveman J., Schiereck P. Absence of photosystem 2 in heterocysts of the blue-green alga Anabaena. Biochim Biophys Acta. 1972 Jan 21;256(1):157–161. doi: 10.1016/0005-2728(72)90170-3. [DOI] [PubMed] [Google Scholar]
- Healey F. P. The Mechanism of Hydrogen Evolution by Chlamydomonas moewusii. Plant Physiol. 1970 Feb;45(2):153–159. doi: 10.1104/pp.45.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao K. K., Rosa L., Hall D. O. Prolonged production of hydrogen gas by a chloroplast biocatalytic system. Biochem Biophys Res Commun. 1976 Jan 12;68(1):21–28. doi: 10.1016/0006-291x(76)90004-8. [DOI] [PubMed] [Google Scholar]
- Stewart W. D., Haystead A., Pearson H. W. Nitrogenase activity in heterocysts of blue-green algae. Nature. 1969 Oct 18;224(5216):226–228. doi: 10.1038/224226a0. [DOI] [PubMed] [Google Scholar]
- Stewart W. D., Rowell P., Tel-or E. Nitrogen fixation and the heterocyst in blue-green algae. Biochem Soc Trans. 1975;3(3):357–361. doi: 10.1042/bst0030357. [DOI] [PubMed] [Google Scholar]
- Thomas J. Absence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga. Nature. 1970 Oct 10;228(5267):181–183. doi: 10.1038/228181b0. [DOI] [PubMed] [Google Scholar]
- Weare N. M., Benemann J. R. Nitrogen fixation by Anabaena cylindrica. II. Nitrogenase activity during induction and aging of batch cultures. Arch Mikrobiol. 1973 Oct 19;93(2):101–112. [PubMed] [Google Scholar]
- Weissman J. C., Benemann J. R. Hydrogen production by nitrogen-starved cultures of Anabaena cylindrica. Appl Environ Microbiol. 1977 Jan;33(1):123–131. doi: 10.1128/aem.33.1.123-131.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winter H. C., Burris R. H. Nitrogenase. Annu Rev Biochem. 1976;45:409–426. doi: 10.1146/annurev.bi.45.070176.002205. [DOI] [PubMed] [Google Scholar]
- Wolk C. P. Movement of carbon from vegetative cells to heterocysts in Anabaena cylindrica. J Bacteriol. 1968 Dec;96(6):2138–2143. doi: 10.1128/jb.96.6.2138-2143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]