Skip to main content
NCBI home page
Gut logoLink to Gut
. 1978 Apr;19(4):267–272. doi: 10.1136/gut.19.4.267

Vitamin D-3 intestinal absorption in vivo: influence of fatty acids, bile salts, and perfusate pH on absorption.

D Hollander, K S Muralidhara, A Zimmerman
PMCID: PMC1411923  PMID: 25826

Abstract

Intestinal absorption of vitamin D-3 in physiological concentrations was studied in the live unanesthetised rat. In both the jejunum and the ileum a linear relationship was found between the absorption rate of the vitamin and its intraluminal concentration. Increasing the sodium taurocholate concentation in the perfusate above 5mM did not change ileal absorption rate but did decrease jejunal absorption rate. The vitamin's rate of absorption was raised by increases in either the hydrogen ion concentration in vivo is mediated by passive diffusion. The rate of absorption of ttion or the perfusate's flow rate. Addition of 2.5 mM fatty acids of varying chain length and degrees of saturation resulted in a decrease in the rate of vitamin D-3 absorption. These experiments indicate that vitamin D-3 absorption in vivo is mediated by passive diffusion. The rate of absorption of the vitamin is influenced by the composition of the perfusate and the thickness of the unstirred layer.

Full text

PDF
267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOOM B., CHAIKOFF I. L., REINHARDT Intestinal lymph as pathway for transport of absorbed fatty acids of different chain lengths. Am J Physiol. 1951 Aug;166(2):451–455. doi: 10.1152/ajplegacy.1951.166.2.451. [DOI] [PubMed] [Google Scholar]
  2. Carey M. C., Small D. M. The characteristics of mixed micellar solutions with particular reference to bile. Am J Med. 1970 Nov;49:590–608. doi: 10.1016/s0002-9343(70)80127-9. [DOI] [PubMed] [Google Scholar]
  3. Clark M. L., Lanz H. C., Senior J. R. Bile salt regulation of fatty acid absorption and esterification in rat everted jejunal sacs in vitro and into thoracic duct lymph in vivo. J Clin Invest. 1969 Sep;48(9):1587–1599. doi: 10.1172/JCI106124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fraser D. R., Kodicek E. Conformational similarities of vitamin D and cholesterol as enzyme substrates. Nature. 1968 Dec 7;220(5171):1031–1032. doi: 10.1038/2201031a0. [DOI] [PubMed] [Google Scholar]
  5. Gregg J. A. New solvent systems for thin-layer chromatography of bile acids. J Lipid Res. 1966 Jul;7(4):579–581. [PubMed] [Google Scholar]
  6. HENDRY E. B. Osmolarity of human serum and of chemical solutions of biologic importance. Clin Chem. 1961 Apr;7:154–164. [PubMed] [Google Scholar]
  7. Hollander D., Muralidhara K. S. Vitamin A1 intestinal absorption in vivo: influence of luminal factors on transport. Am J Physiol. 1977 May;232(5):E471–E477. doi: 10.1152/ajpendo.1977.232.5.E471. [DOI] [PubMed] [Google Scholar]
  8. Hollander D., Rim E., Muralidhara K. S. Vitamin K1 intestinal absorption in vivo: influence of luminal contents on transport. Am J Physiol. 1977 Jan;232(1):E69–E74. doi: 10.1152/ajpendo.1977.232.1.E69. [DOI] [PubMed] [Google Scholar]
  9. Hollander D., Rosenstreich S. J., Volwiler W. Role of the duodenum in vitamin D3 absorption in man. Am J Dig Dis. 1971 Feb;16(2):145–149. doi: 10.1007/BF02284452. [DOI] [PubMed] [Google Scholar]
  10. Hollander D., Truscott T. C. Mechanism and site of small intestinal uptake of vitamin D3 in pharmacological concentrations. Am J Clin Nutr. 1976 Sep;29(9):970–975. doi: 10.1093/ajcn/29.9.970. [DOI] [PubMed] [Google Scholar]
  11. Hollander D., Truscott T. C. Mechanism and site of vitamin K-3 small intestinal transport. Am J Physiol. 1974 Jun;226(6):1516–1522. doi: 10.1152/ajplegacy.1974.226.6.1516. [DOI] [PubMed] [Google Scholar]
  12. Lewis L. D., Fordtran J. S. Effect of perfusion rate on absorption, surface area, unstirred water layer thickness, permeability, and intraluminal pressure in the rat ileum in vivo. Gastroenterology. 1975 Jun;68(6):1509–1516. [PubMed] [Google Scholar]
  13. Miller D. L., Schedl H. P. Total recovery studies of nonabsorbable indicators in the rat small intestine. Gastroenterology. 1970 Jan;58(1):40–46. [PubMed] [Google Scholar]
  14. Saunders D. R. Regional differences in the effect of bile salts on absorption by rat small intestine in vivo. J Physiol. 1975 Sep;250(2):373–383. doi: 10.1113/jphysiol.1975.sp011060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smyth D. H. Methods of studying intestinal absorption. Biomembranes. 1974;4A(0):241–283. [PubMed] [Google Scholar]
  16. Thompson G. R., Ockner R. K., Isselbacher K. J. Effect of mixed micellar lipid on the absorption of cholesterol and vitamin D3 into lymph. J Clin Invest. 1969 Jan;48(1):87–95. doi: 10.1172/JCI105977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Westergaard H., Dietschy J. M. Delineation of the dimensions and permeability characteristics of the two major diffusion barriers to passive mucosal uptake in the rabbit intestine. J Clin Invest. 1974 Sep;54(3):718–732. doi: 10.1172/JCI107810. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES