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. 1977 Jan;74(1):173–177. doi: 10.1073/pnas.74.1.173

Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.

R L Jansing, J L Stein, G S Stein
PMCID: PMC393220  PMID: 264672

Abstract

The regulation of histone gene expression was examined after confluent, nondividing WI-38 human diploid fibroblasts were stimulated to proliferate. Histone mRNA sequences were assayed by hybrid formation with an 3H-labeled single-stranded DNA complementary to histone mRNAs. Histone mRNA became associated with polyribosomes concomitant with the activation of DNA synthesis. The ability of chromatin from WI-38 cells to serve as a template for in vitro transcription of histone mRNA sequences parallels the onset of DNA replication. A role for nonhistone chromosomal proteins in the control of histone gene readout is suggested because, when chromatin from confluent WI-38 cells was dissociated and then reconstituted in the presence of S phase nonhistone chromosomal proteins, a 500-fold activation of histone mRNA sequence transcription was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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