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. 2016 May 27;21(6):691. doi: 10.3390/molecules21060691

Expression of Terpenoid Biosynthetic Genes and Accumulation of Chemical Constituents in Valeriana fauriei

Yun Ji Park 1, Mariadhas Valan Arasu 2, Naif Abdullah Al-Dhabi 2, Soon Sung Lim 3, Yeon Bok Kim 4, Sang Won Lee 4, Sang Un Park 1,*
Editor: Tobias A M Gulder
PMCID: PMC6274141  PMID: 27240331

Abstract

Valeriana fauriei (V. fauriei), which emits a characteristic and unpleasant odor, is important in traditional medicine. In this study, the expression of terpenoid biosynthetic genes was investigated in different organs that were also screened for volatile compounds including valerenic acid and its derivatives. Specific expression patterns from different parts of V. fauriei were observed using quantitative real-time PCR (qRT-PCR). The highest transcript levels of biosynthetic genes involved in mevalonic acid (MVA) and methylerythritol phosphate (MEP) production were found in the stem. Although the amounts of volatile compounds were varied by organ, most of the volatile terpenoids were accumulated in the root. Gas chromatography mass spectrometry (GC-MS) analysis identified 128 volatile compounds, which represented 65.33% to 95.66% of total volatiles. Certain compounds were only found in specific organs. For example, isovalerenic acid and valerenic acid and its derivatives were restricted to the root. Organs with high transcript levels did not necessarily have high levels of the corresponding chemical constituents. According to these results, we hypothesize that translocation may occur between different organs in V. fauriei.

Keywords: gene expression, terpenoid, valerenic acid, Valeriana fauriei, volatile compounds

1. Introduction

Plants have complex mixtures of volatile lipophilic compounds with low molecular weight and high vapor pressure, derived from both primary and secondary metabolisms [1]. More than 1700 volatile compounds have been characterized from 90 plant families [2]. Plants release these compounds for general or specialized functions in both floral and vegetative tissues [3]. These compounds protect against herbivores, pathogens, and parasites, attract pollinators and seed dispersers, and provide plant–plant signaling [4]. Additionally, volatile compounds emitted from plants can seal wounds [5]. For millennia, humans have used floral scents from many aromatic plants, intended to attract pollinators, as sources of flavorings, preservatives, and herbal remedies [6]. Researches have documented antimicrobial, anti-inflammatory, bronchodilatory, expectorant, anticonvulsant, cholagogic, analgesic, and spasmolytic effects of volatile compounds [7]. Volatile compounds determine a plant’s chemotype and can influence its ecological relevance by shaping interactions with pollinators and herbivores [8]. Diverse volatile compounds are synthesized by different biochemical pathways in plants. There are lipoxygenase pathways including oxylipins, green leaf volatiles, isoprene and other terpenoids, some carotenoid derivatives, indoles, and phenolics, such as methyl salicylate [9].

Terpenoids, which constitute the largest class of volatiles from plants, are synthesized from isoprenoid pathways (Figure 1) [1]. There are three basic phases of volatile terpenoid biosynthesis [10]. First, C5-isoprene units are formed from isopentenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate (DMAPP) via two compartmentally separated pathways, the mevalonic acid (MVA) pathway in the cytoplasm and the methylerythritol phosphate (MEP) pathway in the chloroplast. These precursors are then catalyzed by short-chain prenyltransferase to form prenyl diphosphates, including geranyl diphosphate (GPP), farnesyl diphosphate (FPP), and geranylgeranyl pyrophosphate (GGPP) [11,12]. Finally, many terpene synthases (TPS) synthesize prenyl diphosphates and build primary representatives of each type of terpenoid skeleton. Many volatile terpenoids are directly catalyzed by TPS, but others are modified by oxidation, dehydrogenation, acylation, and other types of reactions [10]. The MVA pathway is thought to provide three C5 units for volatile sesquiterpenes (C15), while the MEP pathway gives rise to volatile hemiterpenes (C5), monoterpenes (C10), and diterpenes (C20) [13]. However, metabolic cross talk has been demonstrated, regulated by exchange of isoprenes like IPP between cytosolic and chloroplastic pathways [14].

Figure 1.

Figure 1

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Volatile terpenoid biosynthetic pathways in plants. AACT, Acetoacetyl-CoA thiolase; CMK, 4-(cytidine 5′-diphosphate)-2-C-methyl-d-erythritol kinase; DMAPP, dimethylallyl diphosphate; DXP, 1-deoxy-d-xylulose 5-phosphate; DXR, DXP reductoisomerase; DXS, DXP synthase; FDS, farnesyl diphosphate synthase; FPP, farnesyl diphosphate; GA-3P, glyceraldehyde-3-phosphate; GDS, geranyl diphosphate synthase; GGPP, geranylgeranyl diphosphate; GPP, geranyl diphosphate; HDR, (E)-4-hydroxy-3-methylbut 2-enyl diphosphate reductase; HDS, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase; HMG-CoA, 3-hydroxy-3-methylglutaryl-CoA; HMGR, HMG-CoA reductase; HMGS, HMG-CoA synthase; IDI, isopentenyl diphosphate isomerase; IPP, isopentenyl diphosphate; MCT, 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase; MDS, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase; MEP, 2-C-methyl-D-erythritol 4-phosphate; MK, mevalonate kinase; MVA, mevalonate; MVD, mevalonate diphosphate decarboxylase; PMK, phosphomevalonate kinase; TPS, terpene synthases.

The genus Valeriana L. belongs to Caprifoliaceae (honeysuckle family), and includes approximately 250 perennial herbaceous species with malodorous root stalks [15], and grows in temperate areas around the world [16]. These species have been used as mild sedatives and tranquilizers in traditional medicine in various cultures since ancient times [17]. The rhizomes and roots are thought to induce sedation, promote sleep, and relieve depression and anxiety. Species of Valeriana are also used in the food, beverage, and cosmetic industries because of their unique flavor [18]. Many studies have demonstrated that extracts from Valeriana plants include terpenoids, iridoids, flavonoids, and alkaloids [16]. Valerenic acid and its derivatives accumulate in the roots and rhizomes in significant quantities [19]. Chemical constituents of Valeriana vary by species and with seasonal variation and ecological factors [16,17].

We profiled overall volatile compounds, including valerenic acid and its derivatives, from different tissues of Valeriana fauriei (V. fauriei) grown in South Korea. In addition, we characterized the expression patterns of terpenoid biosynthetic genes and compared them with patterns of volatile compound accumulation.

2. Results

2.1. Transcript Levels of Terpenoid Biosynthetic Genes

We determined differential organ-specific expression patterns of terpenoid biosynthetic genes in V. fauriei using qRT-PCR (quantitative real time polymerase chain reaction). The results are shown in Figure 2 and Figure 3. The height of each bar and the error bar indicate the mean and standard deviation based on three independent measurements. The Y-axis represents transcript levels normalized to 18S expression. Most genes related to the MVA pathway showed the highest expression levels in the stem, except for VfHMGS and VfHMGR. The expression patterns of VfAACT, VfMK, VfPMK, VfMVD, and VfFDS were fairly similar, with the highest levels occurring in the stem, followed by the root and the flower or the leaf. Interestingly, in roots, VfAACT and VfFDS, which are involved in the first and last steps of the MVA pathway, were expressed at similar levels to the stem. Among MVA biosynthetic genes, the expression of VfAACT, VfMK and VfMVD was high relative to 18S, while expression of VfFDS was the lowest relative to 18S. VfIDI, which produces the enzyme that catalyzes the isomerization of IPP to DMAPP in both the MVA and MEP pathways, was expressed most abundantly in the stem, followed by the flower, root, and leaf. According to analyses of MEP biosynthetic gene expression levels, most genes, including VfCMK, VfMDS, VfHDR, and VfGDS, had the highest expression levels in the stem, whereas VfDXR and VfHDS were highly expressed in the leaf. VfMCT had similar expression levels in all organs of V. fauriei. The lowest expression levels of VfDXR, VfMDS, VfHDS, and VfHDR were displayed in the root. In contrast, VfDXS transcripts, which are involved in the start of the MEP biosynthetic pathway, had their highest level in the root. Expression levels of terpenoid backbone biosynthetic genes, which lead to IPP and DMAPP production, were primarily high in stem of V. fauriei.

Figure 2.

Figure 2

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Transcript levels of MVA biosynthetic genes in different organs of V. fauriei (Vf). Genes encode the following enzymes: (a) VfAACT, acetoacetyl-CoA thiolase; (b) VfHMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; (c) VfHMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; (d) VfMK, mevalonate kinase; (e) VfPMK, phosphomevalonate kinase; (f) VfMVD, mevalonate diphosphate decarboxylase; (g) VfFDS, farnesyl diphosphate synthase; and (h) VfIDI, isopentenyl diphosphate isomerase.

Figure 3.

Figure 3

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Transcript levels of MEP biosynthetic genes in different organs from V. fauriei (Vf). Genes encode the following enzymes: (a) VfDXS, 1-deoxy-d-xylulose 5-phosphate synthase; (b) VfDXR, 1-deoxy-d-xylulose 5-phosphate reductoisomerase; (c) VfMCT, 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase; (d) VfCMK, 4-(cytidine 5’-diphosphate)-2-C-methyl-d-erythritol kinase; (e) VfMDS, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase; (f) VfHDS, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase; (g) VfHDR, (E)-4-hydroxy-3-methylbut 2-enyl diphosphate reductase; and (h) VfGDS, geranyl diphosphate synthase.

2.2. Analysis of Volatile Constituents

In total, 128 volatile compounds were separated and identified based on comparison of the mass spectra with the National Institute of Standards and Technology (NIST, Gaithersburg, MD, USA) atomic spectra database, Wiley Registry of Mass Spectral Data, and the related literature (Table 1) [20]. The total yield of the volatile compounds was indicated by the highest amount in the root (95.66%), followed by stem (84.05%), leaf (74.91%), and flower (65.33%). We identified 18 monoterpenes, 34 sesquiterpenes, and 80 other volatile compounds from V. fauriei. Monoterpenes, namely borneol (11, 22.96%), bornyl acetate (13, 19.92%), and camphene (1, 3.33%) were found in high amounts in the roots. Interestingly, β-terpinene (3), myrcene (4), p-cymene (5), and borneol (11) were detected only in the root. The leaf extract was characterized by a high content of endo-borneol (10, 11.48%) and γ-terpinene (7, 3.23%). Sesquiterpenes accumulated primarily in the stem (21.55%) and roots (20.66%). The stem contained the highest amount of the sesquiterpene caryophyllene oxide (34, 17.45%). Seven sesquiterpenes (20–22, 24–26, and 50) were root-specific, while four others (33, 37, 39, and 51) were flower-specific. Isovalerenic acid (53, 3.33%), which produces the strong smell of Valeriana species, was found only in the root of V. fauriei.

Table 1.

Composition of volatile compounds in different organs from V. fauriei.

No. Volatile Compounds RI * Relative Peak Area (%)
Flower Stem Leaf Root
1 n-Hexanal 1.43 0 0 0 0.162 ± 0.004
2 Isovaleric acid 11.96 0 0 0 3.338 ± 0.082
3 Camphene 12.91 0 0 0 3.338 ± 0.082
4 β-Pinene 13.64 0 1.244 ± 0.103 0 0.173 ± 0.0041
5 β-Terpinene 14.51 0 0 0 0.558 ± 0.014
6 Myrcene 15.44 0 0 0 0.465 ± 0.011
7 p-Cymene 17.22 0 0 0 0.085 ± 0.002
8 β-Phellandrene 17.79 1.277 ± 0.111 0 0 0.803 ± 0.020
9 γ-Terpinene 19.09 0.132 ± 0.011 0 3.231 ± 0.262 0.052 ± 0.001
10 Terpinolene 21.35 0 0.901 ± 0.074 0 0.041 ± 0.001
11 Ethyl 3-Hydroxymandelate 21.54 0.197 ± 0.017 0.699 ± 0.058 5.331 ± 0.433 0
12 d-Limonene 22.90 0.282 ± 0.024 0 0 0.082 ± 0.002
13 1,3-Bis-(p-carbamoylmethylphenoxy)-2-propanol 23.18 0.512 ± 0.044 0 0 0
14 endo-Borneol 23.60 0 0.125 ± 0.010 11.482 ± 0.932 2.841 ± 0.069
15 1-(4-methoxyphenyl)imidazzoline-2-thione 24.22 0.564 ± 0.049 0.211 ± 0.017 0 0
16 2,2-Dimethyl-3-heptanone 24.41 0 0 0.561 ± 0.046 0
17 1,2,4-Trizol-3-amene 24.87 0.179 ± 0.016 0 0 0
18 Borneol 25.11 0 0 0 22.96 ± 0.561
19 Benzoic acid 25.35 0 0.966 ± 0.080 0 1.522 ± 0.037
20 Benzothialzole 25.87 2.725 ± 0.237 0 0 0
21 Eudesmol 25.95 0 0.354 ± 0.029 0 0.077 ± 0.002
22 2-Isopropyl-5-methyl anisole 26.47 0.987 ± 0.086 0.497 ± 0.041 0 0
23 Myrtenyl acetate 26.73 0 0 2.226 ± 0.181 1.729 ± 0.042
24 2-Hexanoylfuran 27.27 0.610 ± 0.053 0 0.556 ± 0.045 0.132 ± 0.003
25 Perillaldehyde 27.57 0.834 ± 0.072 0 0 1.071 ± 0.026
26 9-oxo-(2,6-dimethylpehenyl)amide 9-H-Fluorene-4-carboxylic acid 27.94 1.067 ± 0.093 0.241 ± 0.020 21.436 ± 1.741 0.581 ± 0.014
27 Acetic acid 28.31 0.612 ± 0.053 0 0.353 ± 0.029 0.86 ± 0.021
28 α-Gurjunene 29.74 0.640 ± 0.056 0 0 0.133 ± 0.003
29 Bornyl acetate 30.51 0.132 ± 0.011 0 0 19.923 ± 0.487
30 9H-Fluorene-4-carboxylic acid 30.77 0.266 ± 0.023 0.306 ± 0.025 0.253 ± 0.021 0.087 ± 0.002
31 α-Elemene 30.88 0 0 0 0.038 ± 0.001
32 1-Trifluoromethyl-4-(2-emthoxylbenzyloxy)-3-nitro-benzene 31.06 0.298 ± 0.026 0.078 ± 0.006 0 0.029 ± 0.001
33 N-(Cyclohexanecarbonyl)-l-proline isobutyl ester 31.35 0.133 ± 0.012 0 0 0.022 ± 0.001
34 Bromopropylate 31.97 0.461 ± 0.040 0.367 ± 0.030 0 0.318 ± 0.008
35 Methadone N-oxide 32.50 0.286 ± 0.025 0 2.524 ± 0.205 0.509 ± 0.012
36 Quinoline 33.01 0.191 ± 0.017 0.174 ± 0.014 1.510 ± 0.123 1.35 ± 0.033
37 2-Chloro-6-methyl-pyridine 33.40 0.190 ± 0.016 0.226 ± 0.019 0.456 ± 0.037 0.065 ± 0.002
38 Pentadecane 33.51 0.170 ± 0.015 0 0 0.095 ± 0.002
39 4,6-bis(1-Methylethyl)-trans-1,3-dioxane 34.00 0 0.074 ± 0.006 0.751 ± 0.061 0.065 ± 0.002
40 o-Choloroaniline 34.12 0 0.955 ± 0.079 1.886 ± 0.153 0
41 α-Caryophyllene alcohol 34.72 0 0 0 1.272 ± 0.031
42 Isolonhifolan-8-ol 34.81 0.8410.073 0 0.424 ± 0.034 0.209 ± 0.005
43 γ-Elemene 34.93 0 0 0 0.357 ± 0.009
44 Caryophyllene 35.22 0.680 ± 0.059 0 0.205 ± 0.017 0.134 ± 0.003
45 5-(Phenylmethyl)-2-thioxo-4-imidazolidinone 35.37 0 2.235 ± 0.0185 0.434 ± 0.035 0
46 α-Acorenol 36.04 0.284 ± 0.025 0 0 4.025 ± 0.098
47 cis-β-Farnesene 36.18 0 0 0 0.041 ± 0.001
48 Humulene 36.28 0 0 0 0.085 ± 0.002
49 Aromadendrene 36.56 0 0 0 0.741 ± 0.018
50 Alloaromandendrene 36.71 1.162 ± 0.101 0 0 0.066 ± 0.002
51 Ylangene 36.92 0 0.299 ± 0.025 0.779 ± 0.063 2.878 ± 0.070
52 1-(1,5-Dimethyl-4-hexenyl)-4-methyl-benzene 37.13 1.181 ± 0.102 0.297 ± 0.025 0 0.065 ± 0.002
53 β-Ionone 37.39 0.554 ± 0.048 0.179 ± 0.015 0.922 ± 0.075 2.333 ± 0.057
54 Pentadecane 37.62 0 0.523 ± 0.043 0.439 ± 0.036 1.786 ± 0.044
55 N-(1H-1,3-Benzimidazol-2-ylmethyl)-4-methoxy-benzamide 38.31 1.646 ± 0.143 0.047 ± 0.004 0.629 ± 0.051 0.475 ± 0.012
56 Malonic acid 38.50 0 0 1.177 ± 0.096 3.118 ± 0.076
57 β-Bisabolene 38.81 0 0.158 ± 0.013 0.119 ± 0.010 0.54 ± 0.013
58 cis-Sesquisabinene 38.87 2.302 ± 0.200 0.640 ± 0.053 2.530 ± 0.0205 0.527 ± 0.013
59 N-Phenyl-(3-methyl-2-oxiranyl) methyl carbamate 39.03 0 0.362 ± 0.030 0 0.287 ± 0.007
60 Bornyl isovalerate 39.20 1.110 ± 0.096 0.143 ± 0.012 0.674 ± 0.055 0.216 ± 0.005
61 exo-3-Methyl-1,7,7-trimethylbicyclo[2,2,1]hept-2-yl butanoate 39.50 0 0.143 ± 0.012 0 2.464 ± 0.060
62 2.6-Dimethylnon-1-en-3-yn-5-yl valeric acid 39.81 0.708 ± 0.061 0 0 1.249 ± 0.031
63 cis-α-Bisabolene 40.33 0.621 ± 0.054 0 0.533 ± 0.043 0.187 ± 0.005
64 trans-Sesquisabinene 40.50 0 0 0.467 ± 0.038 0.213 ± 0.005
65 1-Phenylthio-3-(1-piperidyl)-propan-2ol 40.61 0.894 ± 0.078 0 0 0.134 ± 0.003
66 6-epi-Shyobunol 40.79 0 0.139 ± 0.011 0 2.376 ± 0.058
67 Photocitral B 40.97 0.779 ± 0.068 0.356 ± 0.029 2.119 ± 0.0172 0.282 ± 0.007
68 N-(4-Methoxyphenyl)-propanamide 41.42 1.247 ± 0.019 0.339 ± 0.028 1.212 ± 0.098 2.537 ± 0.062
69 Menthyl acetate 41.68 0 0.254 ± 0.021 0.901 ± 0.073 0.08 ± 0.002
70 (−)-Isolongifolol methyl ether 42.09 0 0 0 0.207 ± 0.005
71 5,5-Dimethyl-4-(3-methyl-1,3-butadienyl)-1-oxaspiro[2,5]octane 42.20 1.121 ± 0.097 0 0 0.127 ± 0.003
72 3,3-dichlorodihydro-2(3H)-furanone 42.38 0 0 0 0.313 ± 0.008
73 Z-9-Pentadecenol 42.74 2.454 ± 0.213 1.530 ± 0.126 3.563 ± 0.289 0.544 ± 0.013
74 Acetonylacetone dioxime 42.97 0 0.122 ± 0.010 0 0
75 1-Adamantylmethyl 3-methyl-2-butenoate 43.27 1.593 ± 0.138 1.392 ± 0.115 0.223 ± 0.018 0.221 ± 0.005
76 3-(Methylthio)phenyl isothiocyanate 43.89 0 0.527 ± 0.044 0.921 ± 0.075 0.041 ± 0.001
77 1-Adamantylmethyl octanoic acid 44.20 0.780 ± 0.068 11.040 ± 0.912 0 0.123 ± 0.003
78 2-(Methylthio)-benzothialzole 44.43 1.013 ± 0.088 0.300 ± 0.025 0.280 ± 0.023 0.06 ± 0.001
79 3-Methyl-2(3H)-benzothiazolethione 44.74 0.596 ± 0.052 0.439 ± 0.036 0.654 ± 0.053 0.046 ± 0.001
80 Pentanoic acid 45.25 1.072 ± 0.09 0.531 ± 0.044 0.522 ± 0.042 0.165 ± 0.004
81 Terpinyl acetate 45.42 1.550 ± 0.135 3.000 ± 0.248 0 0
82 p-Methoxybenzylazidoformate 46.29 0.975 ± 0.085 2.492 ± 0.206 1.051 ± 0.085 0.439 ± 0.011
83 5-Amino-ethyl ester [1,2,4]triazolo[4.3-a]pyrimidine-6-carboxylate 46.47 0.611 ± 0.053 0.288 ± 0.024 0 0
84 1,3,-Trimethyl-2-hydroxymethyl-3,3-dimethyl-4(3-methylbut-2-eny)-cyclohexene 46.71 1.044 ± 0.091 0.766 ± 0.063 0 0
85 5-Butyl-6-hexyloctahydro-1H-indene 47.14 0.992 ± 0.086 0.229 ± 0.019 0 0
86 4-Hydroxy-2-hydroxymethyl-6-methylpyrimidine 47.47 0.456 ± 0.040 0 0 0
87 Murolan-3,9(11)-diene-10-peroxy 47.76 0.888 ± 0.077 0.355 ± 0.029 0.482 ± 0.039 0.131 ± 0.003
88 Methanone 48.04 0.414 ± 0.036 0.2750.023 0 0
89 8-Chlorooctyl isobutyl carbonate 48.29 0.522 ± 0.045 0.134 ± 0.011 0 0
90 1,2-Pentanediol 48.72 0.719 ± 0.062 0 0 0.031 ± 0.001
91 Bicyclogermacrene 49.21 1.642 ± 0.143 0 0 0
92 Larixone 49.39 0 0.159 ± 0.013 0 0
93 Ursane-3,16-diol 49.70 0.856 ± 0.074 0.921 ± 0.076 0.640 ± 0.052 0.04 ± 0.001
94 Dodecahydro-3,8,8,11a-tetramethyl-5H-3,5a-epoxynaphth[2,1-c]oxepin 49.94 1.248 ± 0.108 0.354 ± 0.029 0 0
95 Hexahydro-5-methyl-1-phenyl-1,3,5-triazine-2-thione 50.27 0 1.515 ± 0.125 0.240 ± 0.019 0
96 (2R,4R)-p-Mentha-6,8-diene-2-hydroperoxide 50.71 0.793 ± 0.069 0.271 ± 0.022 0 0
97 4-(Diethoxyphosphiniyl)butanoic acid 51.18 0.259 ± 0.022 0.246 ± 0.020 0 0
98 2,2-Dimethylpropanoic acid 51.74 0.878 ± 0.076 0.076 ± 0.006 0 0
99 Nerolidol isobutyrate 52.17 0.587 ± 0.051 0.253 ± 0.021 0 0
100 Longifolenaldehyde 52.31 0.510 ± 0.044 0 0 0
101 Caryophyllene oxide 52.67 0.359 ± 0.031 17.454 ± 1.442 0 0
102 Carbamic acid 52.89 0.670 ± 0.058 0.939 ± 0.078 0.220 ± 0.018 0.061 ± 0.001
103 Ptenin-6-carboxylic acid 53.22 1.473 ± 0.127 2.224 ± 0.183 0 0.024 ± 0.001
104 2,4,4-Trimethyl-3-hydroxymethyl-5a-(3-methyl-but-2-enyl)-cyclohexene 53.48 0.218 ± 0.019 0.324 ± 0.027 0 0
105 2-(2-Dodecen-1yl)succinic acid 53.76 0.801 ± 0.070 0.145 ± 0.012 0 0
106 Ginsenol 54.37 0.294 ± 0.026 0.394 ± 0.033 0 0
107 Costunolide 54.55 0 0.114 ± 0.009 0 0
108 1-Formyl-2,2,6-trimethyl-3-(3-methyl-but-2-enyl)-6-cyclohexene 54.95 3.140 ± 0.273 1.125 ± 0.093 0 0
109 4-epi-Cubedol 55.61 0.819 ± 0.071 0.313 ± 0.026 0 0
110 5-(6-Bromodecahydro-2-hydroxy-2,5,5a,8a-tetramethyl-1-naphthalene)-1,2-pentanediol 55.82 0.441 ± 0.038 0 0 0
111 (8S,14)-Cedran-diol 56.00 0.855 ± 0.074 0.914 ± 0.075 0 0
112 Cedrol 56.39 0.379 ± 0.033 0 0 0
113 8-Propoxy cedrane 56.65 0.187 ± 0.016 0.078 ± 0.006 0 0
114 5,6,6-Trimethyl-undeca-3,4-diene-2,10-dione 56.81 0.311 ± 0.027 0.130 ± 0.011 0 0
115 Octahydro [1,2]azaborino[1,2-a][1,2]azaborine 57.53 0.535 ± 0.046 0 0 0
116 2.6-Dimethylnon-1-en-3-yn-5-yl valeric acid 58.56 0.292 ± 0.025 0 0 0
117 Terephthalic acid 58.80 0.174 ± 0.015 7.487 ± 0.618 0 0
118 Adamantane 58.98 0.457 ± 0.040 0.944 ± 0.078 0 0
119 4,7-Methano-3,6,8-methenocyclopent[a]indene 59.80 0.105 ± 0.009 0 0 0
120 4-Dimethylamino-2-methyl-1-phenyl-butan-2-ol 60.11 0.316 ± 0.027 0.376 ± 0.031 0 0
121 (±)cis-3,4-Dimethyl-2-phenyltetrahydro-1,4-thiazine 61.09 0.185 ± 0.016 0 0 0
122 2-Isopropyl-6-phenylnicotinonitrile 62.82 0.251 ± 0.022 0 0 0
123 2-Propenoic acid 64.22 0.242 ± 0.021 0.300 ± 0.025 0 0
124 Hexanedioic acid 64.57 0.476 ± 0.041 7.976 ± 0.659 0 0
125 Methadone N-oxide 65.01 0.115 ± 0.010 0 0 0
126 Isophthalic acid 67.21 0.591 ± 0.051 1.164 ± 0.096 0 0
127 Diisooctyl phthalate 68.01 0.276 ± 0.024 0.420 ± 0.035 0 0
128 Di-(2-methyoxyethyl) Isophthalate 69.18 0 0.113 ± 0.009 0 0
Total 65.33 ± 5.564 84.057 ± 6.932 74.916 ± 6.073 95.661 ± 2.342
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* Retent ion time (min).

2.3. Amounts of Valerenic Acid and Its Derivatives

Valerenic acid and its derivatives, acetoxyvalerenic acid and hydroxyvalerenic acid, were extracted from different organs (flower, stem, leaf, and root) of V. fauriei and analyzed with HPLC. Valerenic acid and its derivatives were detected only in roots (Table 2). However, flowers, stems, and leaves have not shown detectable levels of the three compounds. The root extract had an average of 69.450 μg of valerenic acid per g of dry weight. Acetoxyvalerenic acid (32.234 μg/g dry weight) also had a high accumulation in the root. However, hydroxyvalerenic acid was not detected in any organ.

Table 2.

Amounts of valerenic acid and its derivatives in V. fauriei roots.

Compound Dry Weight (μg/g)
valerenic acid 69.450 ± 0.263
acetoxyvalerenic acid 32.234 ± 0.961
hydroxyvalerenic acid n.d. 1
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Numbers indicate the mean of three replicates ± standard deviation. 1 n.d. = not detected.

3. Discussion

There is considerable interest in chemical composition variation in Valeriana owing to the numerous beneficial properties attributed to consumption of the genus. Previously, the genes involved in volatile terpenoid biosynthesis had not been investigated in different organs of V. fauriei. Our study documents the compositional diversity and amount of volatile compounds, including valerenic acid and its derivatives, and quantifies the expression of genes involved in terpenoid biosynthetic pathways, in different organs of V. fauriei.

Roots had high amounts of volatile compounds but low transcript levels. Evidently, expression levels of terpenoid biosynthetic genes did not correspond with the storage of volatile constituents, indicating that products synthesized in the stem or other organs may be translocated into the root. Earlier experimental studies have found similar results. Plants often transport natural products from a synthesis site to an accumulation site [21]. For example, compounds were synthesized in the stem and leaf, then transported to the root of Astragalus membranaceus Moench [22]. In addition, Lykkesfeldt and Moller [23] have reported the synthesis and translocation of glucosinolates from leaves to seeds during development in Tropaeolum majus L. Moreover, nicotine and caffeine, which are primarily produced roots, are transferred to leaves in Arabidopsis thaliana (L.) Heynh. [24]. Translocation of secondary metabolites from source cells to neighboring cells, or even further to other tissues usually occurs in plants. Volatile terpenes are emitted by the transcript levels of biosynthetic enzymes, and transporter for their emission is not required. However, the strong evidence which demonstrates to deny the presence of broad-specificity transporters for the emission of volatiles has not yet been identified. In addition, the mechanism for the long-distance translocation of volatiles has not been investigated in plants [25]. Therefore, we should carry out more experiments such as feeding of radio labels to V. fauriei tissues to clarify the transport and accumulation of volatile compounds in further study.

Various taxa of Valeriana, including V. officinalis L., V. jatamansi Jones, V. officinalis var. latifolia Miq., V. amurensis P. Smirn. ex Kom., V. fauriei, and V. alternifolia Bunge var. stolonifera A.I. Baranov & Skvortsov, have been the focus of scientific studies [18,26,27]. Several studies have included experiments to isolate and identify chemical constituents including sesquiterpenoids and iridoid glycosides from V. fauriei [26,27]. In our study, we identified 128 volatile compounds in V. fauriei by GC/MS. Although most volatile compounds had high concentrations in the roots, each organ had several distinctive compounds that served as biochemical markers. For example, isovalerenic acid, which causes the malodorous characteristic, was accumulated solely in the root of V. fauriei. In addition, we found that borneol and bornyl acetate were the most abundant monoterpenes in the roots of V. fauriei, in agreement with the results from previous studies. Wang et al. [18] documented that V. officinalis var. latifolia contained the highest amounts of bornyl acetate (23.93%) in the roots. To date, more than 100 members of TPSs have been identified from various plant species [13]. The action of these enzymatic family lead to structurally diverse cyclic and acyclic monoterpenes and sesquiterpenes [28]. Specific monoterpene synthase catalyze from GPP to various skeletons which are the precursors such as olefins, alcohols and diphosphate esters for the biosynthesis of monoterpenes [29]. Also, the enzymatic cyclization in several plants including Salvia, Mentha, Tanacetum, Foeniculum, Pinus, and Citrus species has been reported [30]. Cyclization of linalyl diphosphate derived from GPP occurs to form the α-terpinyl cation, which is the universal monocyclic intermediate. Then, bornane family such as borneol, bornyl acetate, and camphor is converted by hydrolysis, oxidation, and rearrangement of carbocylic skeleton [31]. It has been investigated that bornyl diphosphate synthase acts on production of bornane-type monoterpenes in higher plants [32]. The characteristic compound valerenic acid, along with its derivatives and iridoids, are primarily accumulated in the roots and rhizomes of Valeriana species [20].Similarly, we found that high amounts of valerenic acid and hydroxyvalerenic acid were detected only in the roots of V. fauriei. Navarrete et al. [33] performed quantitative analyses of valerenic acids (valerenic, hydroxyvalerenic, and acetoxyvalerenic) in Valeriana species including V. officinalis, V. edulis Nutt. ex Torr. & A. Gray, V. sitchensis Bong., and V. jatamansi, but only V. officinalis and V. sitchensis accumulated these compounds.

In summary, we analyzed organ specificity in general-common MVA and MEP biosynthesis by measuring transcript levels of related biosynthetic genes. We also measured the accumulation of volatile compounds, and the presence of valerenic acid and its derivatives, in specific organs. We separated and identified 128 volatile compounds in V. fauriei cultivated in South Korea. Volatile compounds were detected in all tested organs, but most of the volatile compounds were stored in the root. In contrast, high levels of gene expression predominantly occurred in the stem. Therefore, terpenoids appear to be translocated after biosynthesis and are stored in specific organs.

4. Materials and Methods

4.1. Plant Materials and Growth Conditions

V. fauriei plants were grown at the experimental station, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Pyeongchang, Gangwon-do, South Korea. The plants were grown outdoors in a field from 2013 to 2014. Leaves, stems, flowers, and roots were collected in July 2014 (Figure 4) and then immediately frozen in liquid nitrogen. Different organs from three individual plants were harvested under the same experimental settings. The materials were stored at −80 °C until they were used.

Figure 4.

Figure 4

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Photographs of two-year-old V. fauriei grown in Pyeongchang, Gangwon-do, South Korea.

4.2. Total RNA Extraction and cDNA Preparation

Total RNA was isolated from each organ using a Total RNA Mini Kit (Geneaid, New Taipei City, Taiwan) following the manufacturer’s instructions using three biological replicates. DNase I (Qiagen, Hilden, North Rhine-Westphalia, Germany) was used to remove genomic DNA. RNA concentration and purity were confirmed with agarose gel electrophoresis and a NanoVue Plus Spectrophotometer (GE Healthcare Bio-Science Corp., Piscataway, NJ, USA). cDNA was synthesized from 1 μg of total RNA using the ReverTra Ace-α Kit (Toyobo, Osaka, Japan).

4.3. qRT-PCR Analysis

All gene specific primers used were designed as previously described by Park et al [34] (Table A1). qRT-PCR was performed with a BIO-RAD CFX96 Real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA) with the 2X Real-Time PCR smart mix (Solgent Co., Ltd. Daejeon, Korea) under the following conditions: initial denaturation at 95 °C for 15 min, 39 cycles of denaturation for 20 s at 95 °C and annealing for 20 s at 72 °C, with a final extension at 72 °C for 10 min. Melting curves were analyzed to verify reaction specificity. Means and standard deviations were calculated from three independent biological replicates for each sample and compared by organ.

4.4. GC and GC-MS

Samples (10 g) of the fresh material (leaves, stems, flowers, and roots) were weighed and placed in vials with 25 mL of headspace. A fused-silica fiber covered with a 75-μm-thick layer of carboxen/polydimethylsiloxane (CAR/PDMS) was used to absorb volatile compounds. It was exposed in the headspace in the vials at 25 °C for 20 min, then removed from vials and introduced directly into the GC injector where thermal desorption of the analysis was performed at 250 °C for 3 min.

GC analysis was carried out with an Agilent 6890N GC mainframe fitted with an HP-5 fused-silica capillary column (30 m × 0.32 mm i.d., 0.25 μm film thickness; Agilent, Santa Clara, CA, USA) and a flame ionization detector (FID). The injector and detector temperatures for each analysis were 250 °C and 280 °C, respectively. The carrier gas was nitrogen, with a flow rate of 1.0 mL/min. The column temperature was maintained at 50 °C for 5 min and then programmed as follows: increase from 50 °C to 260 °C at a rate of 3 °C·min−1, increase from 260 °C to 280 °C at a rate of 10 °C·min−1, and hold at 280 °C for 5 min.

GC-MS analysis was performed on a Polaris Q GC/MS (Thermo Finnigan, Waltham, MA, USA) coupled with an HP-5 fused-silica capillary column (30 m × 0.32 mm i.d., film thickness 0.25 μm; Agilent). Helium was used as the carrier gas at a flow rate of 1.0 mL/min. The mass spectra were obtained with an ionization voltage of 70 eV, trap current of 250 μA, and ion source temperature of 200 °C. The oven temperature program was the same as described previously, and injections were made in splitless mode. Volatile compounds were separated and identified based on comparison of the mass spectra with the National Institute of Standards and Technology (NIST) atomic spectra database, Wiley Registry of Mass Spectral Data, and the related literature. Total ion current chromatograms were recorded in a mass range of 40–400 amu. The analyses were performed using three biological replicates.

4.5. Measurement of Valerenic Acid and Derivatives

All samples were lyophilized at −80 °C for 72 h. Dried samples were ground into a fine powder using a mortar and pestle. One g of powdered sample was extracted with 10 mL of 90% (v/v) methanol at room temperature for 30 min and then centrifuged at 19354 rcf for 10 min. The supernatant was transferred to a new tube. Centrifugation and supernatant transfer were repeated two more times. The final extract was adjusted to 1 mL through evaporation using a LABOROTA 4000 rotary evaporator (Heidolph Instruments GmbH, Schwabach, Bavaria, Germany). The solution was filtered with a 0.45 μm Acrodisc syringe filter (Pall Corp.; Port Washington, NY, USA) for HPLC analysis. HPLC analysis was performed with a Futecs model NS-4000 HPLC apparatus (Daejeon, Korea) and a C18 column (μBondapak™ C18 10 μm 125Å 3.9 × 300 nm column, Waters, Milford, MA, USA). The mobile phase had a gradient prepared from mixtures of acetonitrile and 0.25% phosphoric acid, and the column temperature was maintained at 30 °C. The flow rate was maintained at 0.7 mL/min. Twenty microliters of the solution was injected into the HPLC, and the detection wavelength was set at 221 nm. The analysis was performed using three biological replicates.

4.6. Statistical Analysis

The data for gene expression and valerenic acid and its derivatives contents were analyzed by Microsoft EXCEL (Version. 2010, Microsoft Corporation, Seattle, WA, USA). Statistical analyses for the relative peak areas were conducted using XL-STAT, version 2013 (Addinsoft, NY, USA).

Acknowledgments

This work was carried out with the support of “National Institute of Horticultural and Herbal Science (NIHHS)” Rural Development Administration (RDA), Korea. The authors extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding through the Prolific Research Group (PRG-1437-28).

Abbreviations

The following abbreviations are used in this manuscript:

AACT Acetocetyl-CoA thiolase
CAR/PDMS carboxen/polydimethylsiloxane
CMK 4-(cytidine 5′-diphosphate)-2-C-methyl-d-erythritol kinase
DMAPP dimethylallyl diphosphate
DXP 1-deoxy-d-xylulose 5-phosphate
DXR DXP reductoisomerase
DXS DXP synthase
FDS farndsyl diphosphate synthase
FPP farnesyl diphosphate
GA-3P glyceraldehyde-3-phosphae
GC gas chromatography
GC/MS gas chromatography-mass spectrometry
GDS geranyl diphosphate synthase
GGPP geranyl geranyl diphosphate
GPP geranyl diphosphate
HDR (E)-4-hydroxy-3-methylbut 2-enyl diphosphate reductase
HDS (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase
HMG-CoA 3-hydroxy-3-methylglutaryl-CoA
HMGR HMG-CoA reductase
HMGS HMG-CoA synthase
IDI isopentenyl diphosphate isomerase
IPP isopentenyl diphosphate
MCT 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase
MDS 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase
MEP 2-C-methyl-d-erythritol 4-phosphate
MK mevalonate kinase
MVA mevalonae
MVD mevalonate diphosphate decarboxylase
qRT-PCR quantitative Real-time PCR
PMK phosphomevalonate kinase
TPS terpene synthase
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Appendix

Table A1.

Primers sets used in the experiments and their efficiency (%) for qRT-PCR.

Primer Sequence (5′ to 3′) Amplication (bp) Primer Efficiency (%)
VfAACT-F ATCGGGCATGAAAGCAACCA 129 97.9
VfAACT-R GATCCCTTCCTTGCTTCCGCTA
VfHMGS-F TGGTGGAACTGCAGCATTGTTC 131 98.8
VfHMGS-R CCGCACTGTCTGTGCACACGA
VfHMGR-F TCAGATGCCCTGCCTCTTCC 102 100.2
VfHMGR-R GATCTTCTCGCGCCACCTTG
VfMK-F CGGCGGCTGCGTATTGACT 133 107.1
VfMK-R GCAAATCTCGAGCCCATTGC
VfPMK-F GAGCCGGAATCACAGACGGA 146 93.3
VfPMK-R CTCCACGTCTTTGCGAGGCT
VfMVD-F AATGGAATCGCGCTGAAGGA 105 98
VfMVD-R CACAGCATTCGGCCCAGCAT
VfIDI-F AGCAGATGCAGGCGAAGAGG 111 93.3
VfIDI-R GCCTCGCTTAAGTTCCCGTTCT
VfFDS-F TGATGACGACGGCAAGGAGA 131 94.9
VfFDS-R CACCAACCAAGTGAGCATGCAAGA
VfDXS-F GCCCAATACCACCTGTCGGA 121 95.8
VfDXS-R TGCATCGGTCCACCAATCTG
VfDXR-F AGAACTCCGGTCATTGTGCCA 120 100.1
VfDXR-R CCGCCTCGATCTTTGCAAGTTA
VfMCT-F TCAGTTGCTCTGCAAATGGGAGT 104 102.2
VfMCT-R TCCCATTCTTGTGCCCTTTCC
VfCMK-F GCACCATTGTTGGGATCGGT 113 82.6
VfCMK-R CTCGTTCTCGGCTCGTGTGA
VfMDS-F TGCAGCTACTGCTGCTGTGGA 144 70.6
VfMDS-R GGTATGTTGATGCCGCCGAT
VfHDS-F CTGACAGGCGGGCACAGTTT 134 97.1
VfHDS-R GCCGATTCGCATAGCTCTTCC
VfHDR-F CCGAAGCAATCGGGAAGTTG 112 95
VfHDR-R CGCTCTTGAGTAGCGTCGCA
VfGDS-F TAGCAGTGCTGGCGGGAGAT 145 102.8
VfGDS-R TCGCGCGTCGTACTCATTTG
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Author Contributions

N.A.A-D. and S.U.P. conceived and designed the experiments. Y.J.P., S.S.L., Y.B.K., M.V.A. and S.W.L. performed the experiments and wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

Footnotes

Sample Availability: Not Available.

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