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. 1978 Aug;62(2):262–270. doi: 10.1172/JCI109125

Effects of Methylprednisolone on Hydrogen Ion Absorption in the Canine Stomach

Raphael S K Chung 1,2,3, Michael Field 1,2,3, William Silen 1,2,3
PMCID: PMC371762  PMID: 27530

Abstract

The effect of methylprednisolone (2 mg/kg per day given parenterally for 3 doses, 2 wk or 12 wk) on the permeability of mammalian gastric mucosa to hydrogen ion (H+) was examined with denervated fundic pouches in dogs with antrectomies. Transmucosal electric potential difference (PD) and net fluxes of H+ and Na+ were determined for luminal [H+] from 20 to 160 mM and [Na+] from 1 to 140 mM ([H+] and [Na+] were varied reciprocally). The PD was 50-60 mV lumen negative and was constant over the entire range of Na+ and H+ concentration tested. Net H+ flux varied linearly with [H+]. Extrapolation indicated apparent H+ loss at zero luminal concentration, suggesting a basal HCO3− secretion. Addition of acetylsalicylic acid (ASA) or taurocholate decreased the PD to 30-40 mV and increased threefold the slope of the relation between net H+ flux and [H+] (kH). Calculation of PD-independent permeability constants for H+ (PH) with the Goldman constant field equation indicated that this increase in kH could not be attributed solely to the associated decrease in PD. Prednisolone administered for 3 doses had no effect on either the basal mucosal permeability to H+ or the altered permeability induced by ASA or taurocholate. Chronic administration induced a low rate of basal acid secretion (at 12 wk) but had no effect on either PD or kH. However, the increase in kH and PH that developed upon addition of ASA or taurocholate in chronically treated dogs was more than one and a half times that of controls. These data suggest that prolonged treatment with glucocorticoids increases susceptibility of the gastric mucosa to damage by agents that increase permeability to H+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altamirano M. Backdiffusion of H+ during gastric secretion. Am J Physiol. 1970 Jan;218(1):1–6. doi: 10.1152/ajplegacy.1970.218.1.1. [DOI] [PubMed] [Google Scholar]
  2. Black D. A., Rose G., Brewer D. B. Controlled trial of prednisone in adult patients with the nephrotic syndrome. Br Med J. 1970 Aug 22;3(5720):421–426. doi: 10.1136/bmj.3.5720.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black R. B., Hole D., Rhodes J. Bile damage to the gastric mucosal barrier: the influence of pH and bile acid concentration. Gastroenterology. 1971 Aug;61(2):178–184. [PubMed] [Google Scholar]
  4. Bugajski J., Code C. F., Schlegel J. F. Sodium-hydrogen ion exchange across canine resting gastric mucosa. Am J Physiol. 1972 Apr;222(4):858–863. doi: 10.1152/ajplegacy.1972.222.4.858. [DOI] [PubMed] [Google Scholar]
  5. CODE C. F., HIGGINS J. A., MOLL J. C., ORVIS A. L., SCHOLER J. F. The influence of acid on the gastric absorption of water, sodium and potassium. J Physiol. 1963 Apr;166:110–119. doi: 10.1113/jphysiol.1963.sp007093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chung R. S., Field M., Silen W. Permeability of gastric mucosa to hydrogen and lithium. Gastroenterology. 1973 Apr;64(4):593–598. [PubMed] [Google Scholar]
  7. Chvasta T. E., Cooke A. R. The effect of several ulcerogenic drugs on the canine gastric mucosal barrier. J Lab Clin Med. 1972 Feb;79(2):302–315. [PubMed] [Google Scholar]
  8. Conn H. O., Blitzer B. L. Nonassociation of adrenocorticosteroid therapy and peptic ulcer. N Engl J Med. 1976 Feb 26;294(9):473–479. doi: 10.1056/NEJM197602262940905. [DOI] [PubMed] [Google Scholar]
  9. Cooke A. R., Preshaw R. M., Grossman M. L. Effect of adrenalectomy and glucocorticoids on the secretion and absorption of hydrogen ion. Gastroenterology. 1966 Jun;50(6):761–767. [PubMed] [Google Scholar]
  10. Cooke A. R. Role of adrenocortical steroids in the regulation of gastric secretion. Gastroenterology. 1967 Feb;52(2):272–281. [PubMed] [Google Scholar]
  11. Cushman P., Jr Glucocorticoids and the gastrointestinal tract: current status. Gut. 1970 Jun;11(6):534–539. doi: 10.1136/gut.11.6.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. DAVENPORT H. W. DAMAGE TO THE GASTRIC MUCOSA: EFFECTS OF SALICYLATES AND STIMULATION. Gastroenterology. 1965 Aug;49:189–196. [PubMed] [Google Scholar]
  13. Flemström G. Active alkalinization by amphibian gastric fundic mucosa in vitro. Am J Physiol. 1977 Jul;233(1):E1–12. doi: 10.1152/ajpendo.1977.233.1.E1. [DOI] [PubMed] [Google Scholar]
  14. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. INGLE D. J., PRESTRUD M. C., NEZAMIS J. E. Effects of administering large doses of cortisone acetate to normal rats. Am J Physiol. 1951 Jul;166(1):171–175. doi: 10.1152/ajplegacy.1951.166.1.171. [DOI] [PubMed] [Google Scholar]
  16. Ivey K. J. Gastric mucosal barrier. Gastroenterology. 1971 Aug;61(2):247–257. [PubMed] [Google Scholar]
  17. Kitahara S., Fox K. R., Hogben C. A. Acid secretion, Na+ absorption, and the origin of the potential difference across isolated mammalian stomachs. Am J Dig Dis. 1969 Apr;14(4):221–238. doi: 10.1007/BF02235951. [DOI] [PubMed] [Google Scholar]
  18. Loeb J. N., Sternschein M. J. Suppression of thymidine incorporation into the gastric mucosa of cortisone-treated rats: possible relation to glucocorticoid-induced gastric ulceration. Endocrinology. 1973 May;92(5):1322–1327. doi: 10.1210/endo-92-5-1322. [DOI] [PubMed] [Google Scholar]
  19. Max M., Menguy R. Influence of adrenocorticotropin, cortisone, aspirin, and phenylbutazone on the rate of exfoliation and the rate of renewal of gastric mucosal cells. Gastroenterology. 1970 Mar;58(3):329–336. [PubMed] [Google Scholar]
  20. RASANEN T. Fluctuations in the mitotic frequency of the glandular stomach and intestine of rat under the influence of ACTH. glucocorticoids. stress and heparin. Acta Physiol Scand. 1963 Jun-Jul;58:201–210. doi: 10.1111/j.1748-1716.1963.tb02641.x. [DOI] [PubMed] [Google Scholar]
  21. RASANEN T. Mitotic activity in rat epidermis and gastric mucosa after gluco- and mineralocorticoid administration. Growth. 1962 Mar;26:1–14. [PubMed] [Google Scholar]
  22. Sun D. C. Effect of corticotropin on gastric acid, pepsin, and mucus secretion in dogs with fistulas. Am J Dig Dis. 1969 Feb;14(2):107–112. doi: 10.1007/BF02232833. [DOI] [PubMed] [Google Scholar]
  23. TALALAY P. Enzymic analysis of steroid hormones. Methods Biochem Anal. 1960;8:119–143. doi: 10.1002/9780470110249.ch3. [DOI] [PubMed] [Google Scholar]
  24. TRINDER P. Rapid determination of salicylate in biological fluids. Biochem J. 1954 Jun;57(2):301–303. doi: 10.1042/bj0570301. [DOI] [PMC free article] [PubMed] [Google Scholar]

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