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. 1988 Sep;95(1):183–188. doi: 10.1111/j.1476-5381.1988.tb16563.x

Palytoxin-induced contraction and release of endogenous noradrenaline in rat tail artery.

H Karaki 1, H Nagase 1, Y Ohizumi 1, N Satake 1, S Shibata 1
PMCID: PMC1854150  PMID: 2905903

Abstract

1. The mechanism of the contractile effect of a potent marine toxin, palytoxin (PTX) on the rat isolated tail artery was examined. 2. PTX (10(-7) M) induced a contraction in the tail artery which was dependent on external Ca2+. This contraction was inhibited (by 75% or more) by 10(-6) M prazosin, 2.4 x 10(-5) M bretylium and 10(-4) M 6-hydroxydopamine (6-OHDA), and partially (by 40%) by 10(-5) M indomethacin. However, this contraction was not affected by 10(-6) M tetrodotoxin (TTX), 10(-6) M nifedipine or reserpine treatment. The PTX-induced contraction in reserpine-treated artery was partially inhibited by nifedipine and indomethacin but not by prazosin. 3. Transmural electrical stimulation induced a transient contraction which was dependent on external Ca2+. The contraction induced by electrical stimulation was inhibited by TTX, prazosin, bretylium, reserpine treatment and 6-OHDA but not by nifedipine or indomethacin. 4. PTX increased the release of noradrenaline from this artery. However PTX did not release noradrenaline from reserpine-treated arteries. PTX-induced noradrenaline release was only partially inhibited by TTX or by Ca2+-free solution. 5. These results suggest that PTX has pre- and postsynaptic effects in the rat tail artery. PTX may stimulate adrenergic nerves and release noradrenaline mainly by a TTX-insensitive and Ca2+-independent mechanism and partially by a TTX-sensitive and Ca2+-dependent mechanism. Further, PTX may also release prostaglandins and depolarize smooth muscle cell membrane to induce a contraction.

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Selected References

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  1. Dubois J. M., Cohen J. B. Effect of palytoxin on membrane and potential and current of frog myelinated fibers. J Pharmacol Exp Ther. 1977 Apr;201(1):148–145. [PubMed] [Google Scholar]
  2. Hudgins P. M., Harris T. M. Further studies on the effects of reserpine pretreatment on rabbit aorta: calcium and histologic changes. J Pharmacol Exp Ther. 1970 Dec;175(3):609–618. [PubMed] [Google Scholar]
  3. Ishida Y., Satake N., Habon J., Kitano H., Shibata S. Inhibitory effect of ouabain on the palytoxin-induced contraction of human umbilical artery. J Pharmacol Exp Ther. 1985 Feb;232(2):557–560. [PubMed] [Google Scholar]
  4. Ito K., Karaki H., Ishida Y., Urakawa N., Deguchi T. Effects of palytoxin on isolated intestinal and vascular smooth muscles. Jpn J Pharmacol. 1976 Dec;26(6):683–692. doi: 10.1254/jjp.26.683. [DOI] [PubMed] [Google Scholar]
  5. Ito K., Karaki H., Urakawa N. Effects of palytoxin on mechanical and electrical activities of guinea pig papillary muscle. Jpn J Pharmacol. 1979 Jun;29(3):467–476. doi: 10.1254/jjp.29.467. [DOI] [PubMed] [Google Scholar]
  6. Ito K., Karaki H., Urakawa N. The mode of contractile action of palytoxin on vascular smooth muscle. Eur J Pharmacol. 1977 Nov 1;46(1):9–14. doi: 10.1016/0014-2999(77)90137-6. [DOI] [PubMed] [Google Scholar]
  7. Karaki H., Nakagawa H., Urakawa N. Effects of calcium antagonists on release of [3H]noradrenaline in rabbit aorta. Eur J Pharmacol. 1984 Jun 1;101(3-4):177–183. doi: 10.1016/0014-2999(84)90154-7. [DOI] [PubMed] [Google Scholar]
  8. Karaki H., Weiss G. B. Calcium channels in smooth muscle. Gastroenterology. 1984 Oct;87(4):960–970. [PubMed] [Google Scholar]
  9. Kudo Y., Shibata S. The potent depolarizing action of palytoxin isolated from Palythoa tubercurosa on the isolated spinal cord of the frog. Br J Pharmacol. 1980;71(2):575–579. doi: 10.1111/j.1476-5381.1980.tb10975.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Muramatsu I., Uemura D., Fujiwara M., Narahashi T. Characteristics of palytoxin-induced depolarization in squid axons. J Pharmacol Exp Ther. 1984 Dec;231(3):488–494. [PubMed] [Google Scholar]
  11. Nagase H., Karaki H. Palytoxin-induced contraction and release of prostaglandins and norepinephrine in the aorta. J Pharmacol Exp Ther. 1987 Sep;242(3):1120–1125. [PubMed] [Google Scholar]
  12. Nagase H., Karaki H., Urakawa N. Palytoxin-induced endothelium-dependent relaxation in the isolated rat aorta. Naunyn Schmiedebergs Arch Pharmacol. 1987 May;335(5):575–579. doi: 10.1007/BF00169127. [DOI] [PubMed] [Google Scholar]
  13. Nagase H., Ozaki H., Karaki H., Urakawa N. Intracellular Ca2+-calmodulin system involved in the palytoxin-induced K+ release from rabbit erythrocytes. FEBS Lett. 1986 Jan 20;195(1-2):125–128. doi: 10.1016/0014-5793(86)80145-4. [DOI] [PubMed] [Google Scholar]
  14. Ohizumi Y., Kajiwara A., Yasumoto T. Excitatory effect of the most potent marine toxin, maitotoxin, on the guinea-pig vas deferens. J Pharmacol Exp Ther. 1983 Oct;227(1):199–204. [PubMed] [Google Scholar]
  15. Ohizumi Y., Shibata S. Mechanism of the excitatory action of palytoxin and N-acetylpalytoxin in the isolated guinea-pig vas deferens. J Pharmacol Exp Ther. 1980 Jul;214(1):209–212. [PubMed] [Google Scholar]
  16. Ozaki H., Nagase H., Urakawa N. Involvement of the sugar moiety in the inhibitory action of the cardiac glycosides on the palytoxin-induced responses in vascular smooth muscles. J Pharmacol Exp Ther. 1984 Oct;231(1):153–158. [PubMed] [Google Scholar]
  17. Ozaki H., Tomono J., Nagase H., Urakawa N. The mechanism of contractile action of palytoxin on vascular smooth muscle of guinea-pig aorta. Jpn J Pharmacol. 1983 Dec;33(6):1155–1162. doi: 10.1254/jjp.33.1155. [DOI] [PubMed] [Google Scholar]
  18. Rayner M. D., Sanders B. J., Harris S. M., Lin Y. C., Morton B. E. Palytoxin: effects on contractility and 45Ca2+ uptake in isolated ventricle strips. Res Commun Chem Pathol Pharmacol. 1975 May;11(1):55–64. [PubMed] [Google Scholar]
  19. Shibata S., Satake N., Ueda S., Ohizumi Y., Flores F., Paulino R. The contractile action of palytoxin in the isolated rabbit urinary bladder. Eur J Pharmacol. 1986 Aug 7;127(1-2):129–133. doi: 10.1016/0014-2999(86)90214-1. [DOI] [PubMed] [Google Scholar]
  20. Vanhoutte P. M., Verbeuren T. J., Webb R. C. Local modulation of adrenergic neuroeffector interaction in the blood vessel well. Physiol Rev. 1981 Jan;61(1):151–247. doi: 10.1152/physrev.1981.61.1.151. [DOI] [PubMed] [Google Scholar]

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