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. 1977 Sep 1;146(3):779–791. doi: 10.1084/jem.146.3.779

Cell-mediated mitogenic response induced by leukoagglutinin and Lens culinaris lectin in mouse lymphocytes

PMCID: PMC2180798  PMID: 302308

Abstract

The proliferative response of mouse lymphocytes to syngeneic cellular stimulation upon membrane modification with lectins was studied. Brief pretreatment of stimulator cells (mitomycin-C-treated spleen cells) followed by mixed culture with syngeneic cortisone-resistant thymocytes resulted in a significant proliferative response in the thymocytes. This effect was not due to a soluble mediator and was similar to the mitogenic response after Con A-induced membrane modification reported previously. Because of its general characteristics, we refer to this response as cell-mediated mitogenic response (CMMR). Cell contact between stimulator and responder cells was necessary but not sufficient for the induction of the response. The lectins that generated CMMR were T-cell mitogens. CMMR was generated in all the syngeneic combinations tested and even in allogeneic combinations. No detectable cytotoxic activity towards syngeneic targets cells was produced after CMMR. Moreover, CMMR in allogeneic combinations led to the suppression of the generation of specific cytotoxic lymphocytes. Population analysis with antibodies against T or B cells, nylon wool fractionation of stimulator cells, and tests with peritoneal macrophages and with spleen cells from athymic mice revealed that CMMR depends predominantly on the interaction between responder T cells and stimulator Ig-positive lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Möller G., Sjöberg O. Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972 Aug;4(4):381–393. doi: 10.1016/0008-8749(72)90040-8. [DOI] [PubMed] [Google Scholar]
  2. Beyer C. F., Bowers W. E. Periodate and concanavalin A induce blast transformation of rat lymphocytes by an indirect mechanism. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3590–3593. doi: 10.1073/pnas.72.9.3590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blomgren H., Andersson B. Evidence for a small pool of immunocompetent cells in the mouse thymus. Exp Cell Res. 1969 Oct;57(2):185–192. doi: 10.1016/0014-4827(69)90140-2. [DOI] [PubMed] [Google Scholar]
  4. Burger M. M., Goldberg A. R. Identification of a tumor-specific determinant on neoplastic cell surfaces. Proc Natl Acad Sci U S A. 1967 Feb;57(2):359–366. doi: 10.1073/pnas.57.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cerottini J. C., Nordin A. A., Brunner K. T. Cellular and humoral response to transplantation antigens. I. Development of alloantibody-forming cells and cytotoxic lymphocytes in the graft-versus-host reaction. J Exp Med. 1971 Aug 1;134(2):553–564. doi: 10.1084/jem.134.2.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cheers C., Sprent J. B lymphocytes as stimulators of a mixed lymphocyte reaction. Transplantation. 1973 Mar;15(3):336–337. doi: 10.1097/00007890-197303000-00014. [DOI] [PubMed] [Google Scholar]
  7. Doherty P. C., Zinkernagel R. M. T-cell-mediated immunopathology in viral infections. Transplant Rev. 1974;19(0):89–120. doi: 10.1111/j.1600-065x.1974.tb00129.x. [DOI] [PubMed] [Google Scholar]
  8. Elfenbein G. J., Gelfand M. C. Proliferation of mouse bone marrow-derived lymphocytes in vitro: one mechanism of response to concanavalin A and phytochemagglutinin. Cell Immunol. 1975 Jun;17(2):463–476. doi: 10.1016/s0008-8749(75)80050-5. [DOI] [PubMed] [Google Scholar]
  9. Howard I. K., Sage H. J., Stein M. D., Young N. M., Leon M. A., Dyckes D. F. Studies on a phytohemagglutinin from the lentil. II. Multiple forms of Lens culinaris hemagglutinin. J Biol Chem. 1971 Mar 25;246(6):1590–1595. [PubMed] [Google Scholar]
  10. Jacobsson H., Blomgren H. Characterization of mouse cells releasing or responding to mitogenic factor induced by phytomitogens in vitro. J Immunol. 1975 May;114(5):1631–1637. [PubMed] [Google Scholar]
  11. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  12. Katz D. H., Hamaoka T., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. II. Failure of physiologic cooperative interactions between T and B lymphocytes from allogeneic donor strains in humoral response to hapten-protein conjugates. J Exp Med. 1973 Jun 1;137(6):1405–1418. doi: 10.1084/jem.137.6.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koszinowski U., Ertl H. Lysis mediated by T cells and restricted by H-2 antigen of target cells infected with vaccinia virus. Nature. 1975 Jun 12;255(5509):552–554. doi: 10.1038/255552a0. [DOI] [PubMed] [Google Scholar]
  14. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kyminski J. W., Smith R. T. Evidence for a B-cell -like helper function in mixed lymphocyte culture between immunocompetent thymus cells. J Exp Med. 1975 Feb 1;141(2):360–373. doi: 10.1084/jem.141.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maino V. C., Green N. M., Crumpton M. J. The role of calcium ions in initiating transformation of lymphocytes. Nature. 1974 Sep 27;251(5473):324–327. doi: 10.1038/251324b0. [DOI] [PubMed] [Google Scholar]
  17. Mills G., Monticone V., Paetkau The role of macrophages in thymocyte mitogenesis. J Immunol. 1976 Oct;117(4):1325–1330. [PubMed] [Google Scholar]
  18. Novogrodsky A., Katchalski E. Transformation of neuraminidase-treated lymphocytes by soybean agglutinin. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2515–2518. doi: 10.1073/pnas.70.9.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Novogrodsky A., Lotan R., Ravid A., Sharon N. Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment. J Immunol. 1975 Nov;115(5):1243–1248. [PubMed] [Google Scholar]
  20. Novogrodsky A. Selective activation of mouse T and B lymphocytes by periodate, galactose oxidase and soybean agglutinin. Eur J Immunol. 1974 Oct;4(10):646–648. doi: 10.1002/eji.1830041003. [DOI] [PubMed] [Google Scholar]
  21. O'Brien R. L., Parker J. W., Paolilli P., Steiner J. Periodate-induced lymphocyte transformation. IV. Mitogenic effect of NaIO4 treated lymphocytes upon autologous lymphocytes. J Immunol. 1974 May;112(5):1884–1890. [PubMed] [Google Scholar]
  22. Ozato K., Ebert J. D. Concanavalin A potentiates syngeneic response in murine lymphocytes. J Exp Med. 1976 Jan 1;143(1):1–14. doi: 10.1084/jem.143.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shearer G. M. Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes. Eur J Immunol. 1974 Aug;4(8):527–533. doi: 10.1002/eji.1830040802. [DOI] [PubMed] [Google Scholar]
  26. Skoog V. T., Weber T. H., Richter W. Studies on the interaction between mitogens and human lymphocytes in vitro. Exp Cell Res. 1974 Apr;85(2):339–350. doi: 10.1016/0014-4827(74)90135-9. [DOI] [PubMed] [Google Scholar]
  27. Tichá M., Entlicher G., Kostír J. V., Kocourek J. Studies on phytohemagglutinins. IV. Isolation and characterization of a hemagglutinin from the lentil, Lens, esculenta, Moench. Biochim Biophys Acta. 1970 Nov 17;221(2):282–289. doi: 10.1016/0005-2795(70)90268-0. [DOI] [PubMed] [Google Scholar]
  28. Wong S. Y., Longenecker B. M., Pazderka F., Ruth R. F. Immobilization of lymphocytes at surfaces by lectins. Exp Cell Res. 1975 May;92(2):428–434. doi: 10.1016/0014-4827(75)90398-5. [DOI] [PubMed] [Google Scholar]
  29. Young N. M., Leon M. A., Takahashi T., Howard I. K., Sage H. J. Studies on a phytohemagglutinin from the lentil. 3. Reaction of Lens culinaris hemagglutinin with polysaccharides, glycoproteins, and lymphocytes. J Biol Chem. 1971 Mar 25;246(6):1596–1601. [PubMed] [Google Scholar]

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