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. 1978 Dec;22(3):901–907. doi: 10.1128/iai.22.3.901-907.1978

Phytohemagglutinin stimulation of enhanced immunoglobulin G production in mice inoculated with type III pneumococcal polysaccharide.

S N Vogel, B S Roberson
PMCID: PMC422242  PMID: 32142

Abstract

In BALB/c mice previously inoculated intraperitoneally with an immunogenic dose of the T-independent antigen type III pneumococcal polysaccharide, the intravenous administration of the T-cell activating agent phytohemagglutinin P causes a pronounced increase in the number and relative proportion of immunoglobulin G-producing cells. These results, detected by a modified hemolytic plaque assay, were supported by finding increased levels of serum immunoglobulin G anti-type III pneumococcal polysaccharide in the treated mice. A comparable stimulation of immunoglobulin G antibody-producing cells was not induced in phytohemagglutinin P-treated nude mice, indicating that the change in class of the predominant antibody is attributable to the activation by the phytohemagglutinin P of a T-cell population. Under the conditions of these experiments, phytohemagglutinin P also promotes a progressive suppression of the antibody-forming cells during the response to type III pneumococcal polysaccharide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. H., Stashak P. W. Quantitative and qualitative studies on the primary antibody response to pneumococcal polysaccharides at ehe cellular level. J Immunol. 1969 Dec;103(6):1342–1348. [PubMed] [Google Scholar]
  2. Baker P. J., Barth R. F., Stashak P. W., Amsbaugh D. F. Enhancement of the antibody response to type 3 pneumococcal polysaccharide in mice treated with antilymphocyte serum. J Immunol. 1970 May;104(5):1313–1315. [PubMed] [Google Scholar]
  3. Baker P. J., Stashak P. W., Amsbaugh D. F., Prescott B. Characterization of the antibody response to type 3 pneumococcal polysaccharide at the cellular level. I. Dose-response studies and the effect of prior immunization on the magnitude of the antibody response. Immunology. 1971 Apr;20(4):469–480. [PMC free article] [PubMed] [Google Scholar]
  4. Baker P. J., Stashak P. W., Amsbaugh D. F., Prescott B. Regulation of the antibody response to type 3 pneumococcal polysaccharide. II. Mode of action of thymic-derived suppressor cells. J Immunol. 1974 Jan;112(1):404–409. [PubMed] [Google Scholar]
  5. Baker P. J., Stashak P. W., Prescott B. Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Braley-Mullen H. Regulatory role of T cells in IgG antibody formation and immune memory to type III Pneumococcal polysaccharide. J Immunol. 1974 Dec;113(6):1909–1920. [PubMed] [Google Scholar]
  7. Braley H. C., Freeman M. J. Strain differences in the antibody plaque-forming cell responses of inbred mice to pneumococcal polysaccharide. Cell Immunol. 1971 Feb;2(1):73–81. doi: 10.1016/0008-8749(71)90026-8. [DOI] [PubMed] [Google Scholar]
  8. Coffman R. L., Cohn M. The class of surface immunoglobulin on virgin and memory B lymphocytes. J Immunol. 1977 May;118(5):1806–1815. [PubMed] [Google Scholar]
  9. Coutinho A., Gronowicz E., Bullock W. W., Möller G. Mechanism of thymus-independent immunocyte triggering. Mitogenic activation of B cells results in specific immune responses. J Exp Med. 1974 Jan 1;139(1):74–92. doi: 10.1084/jem.139.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gengozian N., Hübner K. F. Effect of phytohemagglutinin (PHA) on an antibody-forming system. J Immunol. 1967 Jul;99(1):184–190. [PubMed] [Google Scholar]
  11. Gershon R. K. T cell control of antibody production. Contemp Top Immunobiol. 1974;3:1–40. doi: 10.1007/978-1-4684-3045-5_1. [DOI] [PubMed] [Google Scholar]
  12. HUMPHREY J. H., PARROTT D. M., EAST J. STUDIES ON GLOBULIN AND ANTIBODY PRODUCTION IN MICE THYMECTOMIZED AT BIRTH. Immunology. 1964 Jul;7:419–439. [PMC free article] [PubMed] [Google Scholar]
  13. Havas H. F., Hraba T. A study of the kinetics of the anti-dinitrophenyl antibody response at the cellular level. J Immunol. 1969 Aug;103(2):349–356. [PubMed] [Google Scholar]
  14. Howard J. G., Christie G. H., Courtenay B. M., Leuchars E., Davies A. J. Studies on immunological paralysis. VI. Thymic-independence of tolerance and immunity to type 3 pneumococcal polysaccharide. Cell Immunol. 1971 Dec;2(6):614–626. doi: 10.1016/0008-8749(71)90009-8. [DOI] [PubMed] [Google Scholar]
  15. Jones J. M., Amsbaugh D. F., Prescott B. Kinetics of the antibody response to type III pneumococcal polysaccharide (SSS-III). I. Use of 125I-labeled SSS-III to study serum antibody levels, as well as the distribution and excretion of antigen after immunization. J Immunol. 1976 Jan;116(1):41–51. [PubMed] [Google Scholar]
  16. Katz D. H., Benacerraf B. The regulatory influence of activated T cells on B cell responses to antigen. Adv Immunol. 1972;15:1–94. doi: 10.1016/s0065-2776(08)60683-5. [DOI] [PubMed] [Google Scholar]
  17. Kong A. S., Morse S. I. The effect of Bordetella pertussis on the antibody response in mice to type III pneumococcal polysaccharide. J Immunol. 1976 Apr;116(4):989–993. [PubMed] [Google Scholar]
  18. Moutsopoulos H. M., Boehm-Truitt M., Kassan S. S., Chused T. M. Demonstration of activation of B lymphocytes in New Zealand black mice at birth by an immunoradiometric assay for murine IgM. J Immunol. 1977 Nov;119(5):1639–1644. [PubMed] [Google Scholar]
  19. Petrányi G., Jr, Jánossy G., Alföldy P. Effect of phytohaemagglutinin on plaque forming cells in the mouse spleen. Nature. 1969 Jan 4;221(5175):76–78. doi: 10.1038/221076a0. [DOI] [PubMed] [Google Scholar]
  20. Pick E., Feldman J. D. Effect of phytohemagglutinin on hemolytic plaque formation by rat lymphoid cells. Proc Soc Exp Biol Med. 1968 Feb;127(2):524–526. doi: 10.3181/00379727-127-32731. [DOI] [PubMed] [Google Scholar]
  21. Press J. L., Klinman N. R., McDevitt H. O. Expression of Ia antigens on hapten-specific B cells. I. Delineation of B-cell subpopulations. J Exp Med. 1976 Aug 1;144(2):414–427. doi: 10.1084/jem.144.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spreafico F., Lerner E. M., 2nd Suppression of the primary and secondary immune response of the mouse by phytohemagglutinin. J Immunol. 1967 Mar;98(3):407–416. [PubMed] [Google Scholar]

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