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. 1988 Sep;27(2):103–108. doi: 10.1007/BF00200012

Species-specific TNF induction of thymocyte proliferation

M Jane Ehrke 1,✉, Richard L X Ho 1, Kazuyoshi Hori 1
PMCID: PMC11038300  PMID: 3262011

Abstract

Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF concentration in a biphasic manner. Thus, 100 to 1000 units/ml TNF were near optimal while concentrations ≥1,000 units/ml caused apparent down regulation. The effect was abrogated by neutralizing antibody to rMu-TNF but not by neutralizing antibody to rMu-interleukin 1α or β. The rMu-TNF did not induce proliferation of the mature murine T-helper cell line, D10.G4.1, in the presence of mitogen. Taken together the results indicate that TNF, in a strictly species-specific manner, can regulate thymocyte proliferation independently of interleukin 1.

Keywords: Cancer Research, Tumor Necrosis, Tumor Necrosis Factor, Human Tumor Necrosis Factor, Biphasic Manner

Footnotes

Supported in part by Asahi Chemical Industry Co., Inc. and by USPHS Grants CA-24538, CA-15142 and CA-09072 awarded by the National Cancer Institute, Department of Health and Human Services

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