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. 1988 Jun;56(6):1655–1657. doi: 10.1128/iai.56.6.1655-1657.1988

Cloning and expression of secreted antigens of Clostridium difficile in Escherichia coli.

D C Dailey 1, R H Schloemer 1
PMCID: PMC259452  PMID: 3286505

Abstract

The feasibility of the cloning and expression of Clostridium difficile antigens in Escherichia coli was investigated. The expression of a limited number of cloned clostridial antigens under the control of clostridial promoter elements in E. coli was observed.

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Selected References

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  1. Banno Y., Kobayashi T., Kono H., Watanabe K., Ueno K., Nozawa Y. Biochemical characterization and biologic actions of two toxins (D-1 and D-2) from Clostridium difficile. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S11–S20. doi: 10.1093/clinids/6.supplement_1.s11. [DOI] [PubMed] [Google Scholar]
  2. Bartlett J. G., Chang T., Taylor N. S., Onderdonk A. B. Colitis induced by Clostridium difficile. Rev Infect Dis. 1979 Mar-Apr;1(2):370–378. doi: 10.1093/clinids/1.2.370. [DOI] [PubMed] [Google Scholar]
  3. Bartlett J. G., Moon N., Chang T. W., Taylor N., Onderdonk A. B. Role of Clostridium difficile in antibiotic-associated pseudomembranous colitis. Gastroenterology. 1978 Nov;75(5):778–782. [PubMed] [Google Scholar]
  4. Czuprynski C. J., Johnson W. J., Balish E., Wilkins T. Pseudomembranous colitis in Clostridium difficile-monoassociated rats. Infect Immun. 1983 Mar;39(3):1368–1376. doi: 10.1128/iai.39.3.1368-1376.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engleberg N. C., Drutz D. J., Eisenstein B. I. Cloning and expression of Legionella pneumophila antigens in Escherichia coli. Infect Immun. 1984 May;44(2):222–227. doi: 10.1128/iai.44.2.222-227.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. George W. L. Antimicrobial agent-associated colitis and diarrhea: historical background and clinical aspects. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S208–S213. doi: 10.1093/clinids/6.supplement_1.s208. [DOI] [PubMed] [Google Scholar]
  7. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  8. Justus P. G., Martin J. L., Goldberg D. A., Taylor N. S., Bartlett J. G., Alexander R. W., Mathias J. R. Myoelectric effects of Clostridium difficile: motility-altering factors distinct from its cytotoxin and enterotoxin in rabbits. Gastroenterology. 1982 Oct;83(4):836–843. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lyerly D. M., Saum K. E., MacDonald D. K., Wilkins T. D. Effects of Clostridium difficile toxins given intragastrically to animals. Infect Immun. 1985 Feb;47(2):349–352. doi: 10.1128/iai.47.2.349-352.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Onderdonk A. B., Cisneros R. L., Bartlett J. G. Clostridium difficile in gnotobiotic mice. Infect Immun. 1980 Apr;28(1):277–282. doi: 10.1128/iai.28.1.277-282.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rolfe R. D., Finegold S. M. Purification and characterization of Clostridium difficile toxin. Infect Immun. 1979 Jul;25(1):191–201. doi: 10.1128/iai.25.1.191-201.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rothman S. W. Presence of Clostridium difficile toxin in guinea pigs with penicillin-associated colitis. Med Microbiol Immunol. 1981;169(3):187–196. doi: 10.1007/BF02123592. [DOI] [PubMed] [Google Scholar]
  14. Silva J., Fekety R., Werk C., Ebright J., Cudmore M., Batts D., Syrjamaki C., Lukens J. Inciting and etiologic agents of colitis. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S214–S221. doi: 10.1093/clinids/6.supplement_1.s214. [DOI] [PubMed] [Google Scholar]
  15. Sullivan N. M., Pellett S., Wilkins T. D. Purification and characterization of toxins A and B of Clostridium difficile. Infect Immun. 1982 Mar;35(3):1032–1040. doi: 10.1128/iai.35.3.1032-1040.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]

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