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. 1988 Jan 1;249(1):297–300. doi: 10.1042/bj2490297

Histidine decarboxylase from rat mast cells. Enhanced recovery in cell-free extracts and isotopic labelling.

R S Feldberg 1, D A Iannitti 1, D E Cochrane 1
PMCID: PMC1148697  PMID: 3342012

Abstract

A procedure for obtaining rat mast-cell histidine decarboxylase in greater than 50% yield in cell-free extracts was developed. The enzyme was found in the supernatant fractions from a 3,500 g and a 105,000 g centrifugation step and was demonstrated to be sensitive to inhibition by alpha-fluoromethylhistidine but not by phenylalanine. Although the enzyme shows a half-life of only 3 h in cell-free extract, the initial high recovery of activity allowed for active-site labelling of the enzyme by [3H]histidine and NaBH4. Labelled protein migrated on non-denaturing polyacrylamide-gradient-gel electrophoresis as a 55,000 Da species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaven M. A. Histamine (second of two parts). N Engl J Med. 1976 Feb 5;294(6):320–325. doi: 10.1056/NEJM197602052940608. [DOI] [PubMed] [Google Scholar]
  2. Beaven M. A., Roderick N. B., Shaff R. E., Soll A. H. Histamine synthesis in intact and disrupted rat mast cells. Biochem Pharmacol. 1982 Apr 1;31(7):1189–1195. doi: 10.1016/0006-2952(82)90003-x. [DOI] [PubMed] [Google Scholar]
  3. Beaven M. A., Wilcox G., Terpstra G. K. A microprocedure for the measurement of 14CO2 release from [14C]carboxyl-labeled amino acids. Anal Biochem. 1978 Feb;84(2):638–641. doi: 10.1016/0003-2697(78)90089-1. [DOI] [PubMed] [Google Scholar]
  4. Bibb P. C., Cochrane D. E., Morel-Laurens N. Loss of quin 2 accompanies degranulation of mast cells. FEBS Lett. 1986 Dec 15;209(2):169–174. doi: 10.1016/0014-5793(86)81105-x. [DOI] [PubMed] [Google Scholar]
  5. Cochrane D. E., Douglas W. W. Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48-80 or the ionophores A-23187 and X-537A. Proc Natl Acad Sci U S A. 1974 Feb;71(2):408–412. doi: 10.1073/pnas.71.2.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayashi H., Tanase S., Snell E. E. Pyridoxal 5'-phosphate-dependent histidine decarboxylase. Inactivation by alpha-fluoromethylhistidine and comparative sequences at the inhibitor- and coenzyme-binding sites. J Biol Chem. 1986 Aug 25;261(24):11003–11009. [PubMed] [Google Scholar]
  7. Kollonitsch J., Perkins L. M., Patchett A. A., Doldouras G. A., Marburg S., Duggan D. E., Maycock A. L., Aster S. D. Selective inhibitors of biosynthesis of aminergic neurotransmitters. Nature. 1978 Aug 31;274(5674):906–908. doi: 10.1038/274906a0. [DOI] [PubMed] [Google Scholar]
  8. Kubota H., Hayashi H., Watanabe T., Taguchi Y., Wada H. Mechanism of inactivation of mammalian L-histidine decarboxylase by (S)-alpha-fluoromethylhistidine. Biochem Pharmacol. 1984 Apr 1;33(7):983–990. doi: 10.1016/0006-2952(84)90504-5. [DOI] [PubMed] [Google Scholar]
  9. Martin S. A., Bishop J. O. Purification and characterization of histidine decarboxylase from mouse kidney. Biochem J. 1986 Mar 1;234(2):349–354. doi: 10.1042/bj2340349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schwartz J. C. Histaminergic mechanisms in brain. Annu Rev Pharmacol Toxicol. 1977;17:325–339. doi: 10.1146/annurev.pa.17.040177.001545. [DOI] [PubMed] [Google Scholar]
  11. Schwartz L. B., Austen K. F. Structure and function of the chemical mediators of mast cells. Prog Allergy. 1984;34:271–321. [PubMed] [Google Scholar]
  12. Slutsky B., Jarvis D., Bibb P., Feldberg R. S., Cochrane D. E. Viability and recovery from degranulation of isolated rat peritoneal mast cells. Exp Cell Res. 1987 Jan;168(1):63–78. doi: 10.1016/0014-4827(87)90416-2. [DOI] [PubMed] [Google Scholar]
  13. Taguchi Y., Watanabe T., Kubota H., Hayashi H., Wada H. Purification of histidine decarboxylase from the liver of fetal rats and its immunochemical and immunohistochemical characterization. J Biol Chem. 1984 Apr 25;259(8):5214–5221. [PubMed] [Google Scholar]
  14. Yamada M., Watanabe T., Harino S., Fukui H., Wada H. The effects of protease inhibitors on histidine decarboxylase activities and assay of enzyme in various rat tissues. Biochim Biophys Acta. 1980 Oct;615(2):458–464. doi: 10.1016/0005-2744(80)90511-2. [DOI] [PubMed] [Google Scholar]

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