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. 1977 Dec;34(6):797–800. doi: 10.1128/aem.34.6.797-800.1977

Beneficial effects of catalase or pyruvate in a most-probable-number technique for the detection of Staphylococcus aureus.

D G Brewer, S E Martin, Z J Ordal
PMCID: PMC242750  PMID: 339836

Abstract

The effects of the addition of catalase (EC 1.11.1.6) or pyruvate on the enumeration of Staphylococcus aureus in Trypticase soy broth with 10% NaCl were examined using a most-probable-number technique. Addition of catalase or pyruvate to the broth increased enumeration of all heat-stressed S. aureus strains tested. Increases were also observed with nonstressed cells. Catalase and pyruvate were similarly effective when added to Trypticase soy broth-10% NaCl in enumerating staphylococci naturally present in low-temperature-rendered ground-beef samples.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allwood M. C., Russell A. D. Mechanisms of thermal injury in nonsporulating bacteria. Adv Appl Microbiol. 1970;12:89–119. doi: 10.1016/s0065-2164(08)70583-5. [DOI] [PubMed] [Google Scholar]
  2. Baer E. F., Gilden M. M., Wienke C. L., Mellitz M. B. Comparative efficiency of two enrichment and four plating media for isolation of Staphylococcus aureus. J Assoc Off Anal Chem. 1971 May;54(3):736–738. [PubMed] [Google Scholar]
  3. Bairdparker A. C., Davenport E. The effect of recovery medium on the isolation of Staphylococcus aureus after heat treatment and after the storage of frozen or dried cells. J Appl Bacteriol. 1965 Dec;28(3):390–402. doi: 10.1111/j.1365-2672.1965.tb02169.x. [DOI] [PubMed] [Google Scholar]
  4. Flowers R. S., Martin S. E., Brewer D. G., Ordal Z. J. Catalase and enumeration of stressed Staphylococcus aureus cells. Appl Environ Microbiol. 1977 May;33(5):1112–1117. doi: 10.1128/aem.33.5.1112-1117.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giolitti G., Cantoni C. A medium for the isolation of staphylococci from foodstuffs. J Appl Bacteriol. 1966 Aug;29(2):395–398. doi: 10.1111/j.1365-2672.1966.tb03488.x. [DOI] [PubMed] [Google Scholar]
  6. Gomez R., Takano M., Sinskey A. J. Proceedings: Characteristics of freeze-dried cells. Cryobiology. 1973 Nov;10(5):368–374. doi: 10.1016/0011-2240(73)90060-6. [DOI] [PubMed] [Google Scholar]
  7. HEIMETS F., TAYLOR W. W., LEHMAN J. J. The use of metabolites in the restoration of the viability of heat and chemically inactivated Escherichia coli. J Bacteriol. 1954 Jan;67(1):5–12. doi: 10.1128/jb.67.1.5-12.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harmon S. M., Kautter D. A. Beneficial effect of catalase treatment on growth of Clostridium perfringens. Appl Environ Microbiol. 1976 Sep;32(3):409–416. doi: 10.1128/aem.32.3.409-416.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hurst A., Hendry G. S., Hughes A., Paley B. Enumeration of sublethally heated staphylococci in some dried foods. Can J Microbiol. 1976 May;22(5):677–683. doi: 10.1139/m76-100. [DOI] [PubMed] [Google Scholar]
  10. Iandolo J. J., Ordal Z. J. Repair of thermal injury of Staphylococcus aureus. J Bacteriol. 1966 Jan;91(1):134–142. doi: 10.1128/jb.91.1.134-142.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin S. E., Flowers R. S., Ordal Z. J. Catalase: its effect on microbial enumeration. Appl Environ Microbiol. 1976 Nov;32(5):731–734. doi: 10.1128/aem.32.5.731-734.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Patterson J. T. Comparison of plating and most probable number techniques for the isolation of staphylococci from foods. J Appl Bacteriol. 1973 Jun;36(2):273–277. doi: 10.1111/j.1365-2672.1973.tb04102.x. [DOI] [PubMed] [Google Scholar]

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