Abstract
By the direct immunofluorescence technique 19 liver biopsies were examined for the presence of the e-antigen associated with the hepatitis B virus infection. With the use of double incubation and blocking experiments with FITC and Rhodamine labelled antisera against hepatitis B core antigen and e-antigen, evidence is presented that the e-antigen is localised in the nuclei of the hepatocytes. We conclude that the e-antigen and the core antigen are not identical, although they are often present simultaneously in the nucleus of the hepatocyte.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arnold W., von Mayersbach H. Changes in the solubility of immunoglobulins after fluorescent labeling and its influence on immunofluorescent techniques. J Histochem Cytochem. 1972 Dec;20(12):975–985. doi: 10.1177/20.12.975. [DOI] [PubMed] [Google Scholar]
- Hess G., Nielsen J. O., Arnold W., Büschenfelde K. H. e System and intrahepatocelullar HBcAG and HBsAG in HBsAG positive patients with liver diseases and healthy carriers. Scand J Gastroenterol. 1977;12(3):325–330. doi: 10.3109/00365527709180935. [DOI] [PubMed] [Google Scholar]
- Magnius L. O., Espmark A. A new antigen complex co-occurring with Australia antigen. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):335–337. doi: 10.1111/j.1699-0463.1972.tb00167.x. [DOI] [PubMed] [Google Scholar]
- Magnius L. O., Espmark J. A. New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J Immunol. 1972 Nov;109(5):1017–1021. [PubMed] [Google Scholar]
- Nielsen J. O., Dietrichson O., Juhl E. Incidence and meaning of the "e" determinant among hepatitis-B-antigen positive patients with acute and chronic liver diseases. Report from the Copenhagen Hepatitis Acuta Programme. Lancet. 1974 Oct 19;2(7886):913–915. doi: 10.1016/s0140-6736(74)91127-1. [DOI] [PubMed] [Google Scholar]
- Trepo C., Vitvitski L., Neurath R., Hashimoto N., Schaefer R., Nemoz G. Letter: Detection of e antigen by immunofluorescence in cytoplasm of hepatocytes of HBsAg carriers. Lancet. 1976 Feb 28;1(7957):486–486. doi: 10.1016/s0140-6736(76)91510-5. [DOI] [PubMed] [Google Scholar]