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. 1978 Jul;23(1):15–31. doi: 10.1016/S0006-3495(78)85429-0

Quasi-elastic light scattering from migrating chemotactic bands of Escherichia coli.

M Holz, S H Chen
PMCID: PMC1473557  PMID: 352425

Abstract

We report the observation of migrating chemotactic bands of Escherichia coli in a buffer solution. The temporal development of the bacterial density profile is observed by the scattered light intensity as the band migrates through a stationary laser beam. We have made a preliminary analysis of the observed band profile with help of the Keller-Segel theory. The model accounts for only some aspects of the observed time evolution of the density profile. The microscopic motility characteristics of the E. coli in the band are simultaneously studied by photon correlation. The measured correlation functions are analyzed to obtain the spatial dependence of the half-width within the band. A simple analytical model is proposed to account for the contribution of the twiddle motion to the correlation function. By analyzing the correlation function as a superposition of straight-line and twiddle motions, we obtain a satisfactory agreement between the theory and the measured angular dependence of the line shape. As a consequence we are able to extract a parameter beta, which measures the average fraction of twiddling bacteria in the center of the band at a given time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. Chemotaxis in bacteria. Science. 1966 Aug 12;153(3737):708–716. doi: 10.1126/science.153.3737.708. [DOI] [PubMed] [Google Scholar]
  2. Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. doi: 10.1146/annurev.bi.44.070175.002013. [DOI] [PubMed] [Google Scholar]
  3. Adler J., Dahl M. M. A method for measuring the motility of bacteria and for comparing random and non-random motility. J Gen Microbiol. 1967 Feb;46(2):161–173. doi: 10.1099/00221287-46-2-161. [DOI] [PubMed] [Google Scholar]
  4. Adler J., Templeton B. The effect of environmental conditions on the motility of Escherichia coli. J Gen Microbiol. 1967 Feb;46(2):175–184. doi: 10.1099/00221287-46-2-175. [DOI] [PubMed] [Google Scholar]
  5. Berg H. C., Brown D. A. Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972 Oct 27;239(5374):500–504. doi: 10.1038/239500a0. [DOI] [PubMed] [Google Scholar]
  6. Berg H. C. Chemotaxis in bacteria. Annu Rev Biophys Bioeng. 1975;4(00):119–136. doi: 10.1146/annurev.bb.04.060175.001003. [DOI] [PubMed] [Google Scholar]
  7. Boon J. P., Nossal R., Chien S. H. Light-scattering spectrum due to wiggling motions of bacteria. Biophys J. 1974 Nov;14(11):847–864. doi: 10.1016/s0006-3495(74)85954-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahlquist F. W., Lovely P., Koshland D. E., Jr Quantitative analysis of bacterial migration in chemotaxis. Nat New Biol. 1972 Mar 29;236(65):120–123. doi: 10.1038/newbio236120a0. [DOI] [PubMed] [Google Scholar]
  9. Keller E. F., Segel L. A. Traveling bands of chemotactic bacteria: a theoretical analysis. J Theor Biol. 1971 Feb;30(2):235–248. doi: 10.1016/0022-5193(71)90051-8. [DOI] [PubMed] [Google Scholar]
  10. Koshland D. E., Jr A response regulator model in a simple sensory system. Science. 1977 Jun 3;196(4294):1055–1063. doi: 10.1126/science.870969. [DOI] [PubMed] [Google Scholar]
  11. Lovely P. S., Dahlquist F. W. Statistical measures of bacterial motility and chemotaxis. J Theor Biol. 1975 Apr;50(2):477–496. doi: 10.1016/0022-5193(75)90094-6. [DOI] [PubMed] [Google Scholar]
  12. Macnab R. M., Koshland D. E., Jr The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509–2512. doi: 10.1073/pnas.69.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCabe M., Laurent T. C. Diffucison of OXYGEN, Nitrogen and water in hyluronate solutions. Biochim Biophys Acta. 1975 Jul 14;399(1):131–138. doi: 10.1016/0304-4165(75)90219-6. [DOI] [PubMed] [Google Scholar]
  14. Nossal R., Chen S. H. Effects of chemoattractants on the motility of Escherichia coli. Nat New Biol. 1973 Aug 22;244(138):253–254. doi: 10.1038/newbio244253a0. [DOI] [PubMed] [Google Scholar]
  15. Nossal R. Growth and movement of rings of chemotactic bacteria. Exp Cell Res. 1972 Nov;75(1):138–142. doi: 10.1016/0014-4827(72)90529-0. [DOI] [PubMed] [Google Scholar]
  16. Nossal R., Weiss G. H. Analysis of a densitometry assay for bacterial chemotaxis. J Theor Biol. 1973 Sep 14;41(1):143–147. doi: 10.1016/0022-5193(73)90194-x. [DOI] [PubMed] [Google Scholar]
  17. Schaefer D. W., Banks G., Alpert S. S. Intensity fluctuation spectroscopy of motile microorganisms. Nature. 1974 Mar 8;248(5444):162–164. doi: 10.1038/248162a0. [DOI] [PubMed] [Google Scholar]
  18. Scribner T. L., Segel L. A., Rogers E. H. A numerical study of the formation and propagation of traveling bands of chemotactic bacteria. J Theor Biol. 1974 Jul;46(1):189–219. doi: 10.1016/0022-5193(74)90147-7. [DOI] [PubMed] [Google Scholar]
  19. Springer M. S., Kort E. N., Larsen S. H., Ordal G. W., Reader R. W., Adler J. Role of methionine in bacterial chemotaxis: requirement for tumbling and involvement in information processing. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4640–4644. doi: 10.1073/pnas.72.11.4640. [DOI] [PMC free article] [PubMed] [Google Scholar]

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