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. 1987 Mar;55(3):259–263. doi: 10.1038/bjc.1987.50

Effects of in vivo modulation of splenic natural killer cell activity on the growth of spleen-seeking tumour variants.

M A Skinner, K Thompson, T Ezaki, J Marbrook
PMCID: PMC2001739  PMID: 3552015

Abstract

A novel tumour system has been used to study the effect of natural killer cells on tumour growth by using agents which modify natural killer cell activity. The tumour cells are hybridoma cells which secrete antibody specific for red blood cells so that tumour growth can be quantitated by a haemolytic plaque assay. Spleen-seeking variants have been derived from original hybrids which are sensitive to natural killer cells. Treatment of mice with polyinosinic-polycytidylic acid substantially enhanced natural killer cell activity and correlated closely with a reduction in the growth of the hybridoma tumour cells in the spleen and life extension. Conversely, a single injection of anti-asialo GM, antibody resulted in a substantial increase in the number of plaque forming splenic tumour cells and virtual elimination of natural killer cell activity. These data demonstrate the important role of natural killer cells in constraining the growth of a tumour of B cell origin and establishes the usefulness of this tumour model in studying the biology of effects on tumour growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Djeu J. Y., Heinbaugh J. A., Holden H. T., Herberman R. B. Augmentation of mouse natural killer cell activity by interferon and interferon inducers. J Immunol. 1979 Jan;122(1):175–181. [PubMed] [Google Scholar]
  2. Ezaki T., Christensen N. D., Skinner M. A., Marbrook J. The adaptation of the plaque reduction assay for measuring specific cytotoxic cells in limiting dilution cultures. J Immunol Methods. 1984 Feb 10;66(2):357–368. doi: 10.1016/0022-1759(84)90349-1. [DOI] [PubMed] [Google Scholar]
  3. Ezaki T., Marbrook J. The use of hybridoma cells as a murine model to study tumor immunity. Int J Cancer. 1985 Jan 15;35(1):107–111. doi: 10.1002/ijc.2910350117. [DOI] [PubMed] [Google Scholar]
  4. Ezaki T., Skinner M. A., Marbrook J. Spontaneous cytotoxic T cells in murine spleen-cell cultures. II. Distinguishing between spontaneous cytotoxic T cells and NK cells according to kinetics and target selectivity. Immunology. 1983 Nov;50(3):351–357. [PMC free article] [PubMed] [Google Scholar]
  5. Gidlund M., Orn A., Wigzell H., Senik A., Gresser I. Enhanced NK cell activity in mice injected with interferon and interferon inducers. Nature. 1978 Jun 29;273(5665):759–761. doi: 10.1038/273759a0. [DOI] [PubMed] [Google Scholar]
  6. Gorelik E., Wiltrout R. H., Okumura K., Habu S., Herberman R. B. Role of NK cells in the control of metastatic spread and growth of tumor cells in mice. Int J Cancer. 1982 Jul 15;30(1):107–112. doi: 10.1002/ijc.2910300118. [DOI] [PubMed] [Google Scholar]
  7. Gresser I., Tovey M. G. Antitumor effects of interferon. Biochim Biophys Acta. 1978 Oct 27;516(2):231–247. doi: 10.1016/0304-419x(78)90009-4. [DOI] [PubMed] [Google Scholar]
  8. Grimm E. A., Mazumder A., Zhang H. Z., Rosenberg S. A. Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes. J Exp Med. 1982 Jun 1;155(6):1823–1841. doi: 10.1084/jem.155.6.1823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanna N., Burton R. C. Definitive evidence that natural killer (NK) cells inhibit experimental tumor metastases in vivo. J Immunol. 1981 Nov;127(5):1754–1758. [PubMed] [Google Scholar]
  10. Hanna N., Fidler I. J. Expression of metastatic potential of allogenic and xenogeneic neoplasms in young nude mice. Cancer Res. 1981 Feb;41(2):438–444. [PubMed] [Google Scholar]
  11. Herberman R. B., Nunn M. E., Lavrin D. H. Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic acid allogeneic tumors. I. Distribution of reactivity and specificity. Int J Cancer. 1975 Aug 15;16(2):216–229. doi: 10.1002/ijc.2910160204. [DOI] [PubMed] [Google Scholar]
  12. Kawase I., Urdal D. L., Brooks C. G., Henney C. S. Selective depletion of NK cell activity in vivo and its effect on the growth of NK-sensitive and NK-resistant tumor cell variants. Int J Cancer. 1982 May 15;29(5):567–574. doi: 10.1002/ijc.2910290513. [DOI] [PubMed] [Google Scholar]
  13. Moore M. Natural immunity to tumours--theoretical predictions and biological observations. Br J Cancer. 1985 Aug;52(2):147–151. doi: 10.1038/bjc.1985.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nolibe D., Aumaitre E., Thang M. N. In vivo augmentation of rat lung natural killer cell activity and inhibition of experimental metastases by double-stranded polynucleotides. Cancer Res. 1985 Oct;45(10):4774–4778. [PubMed] [Google Scholar]
  15. Ruggeri Z. M., De Marco L., Gatti L., Bader R., Montgomery R. R. Platelets have more than one binding site for von Willebrand factor. J Clin Invest. 1983 Jul;72(1):1–12. doi: 10.1172/JCI110946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Skinner M. A., Marbrook J. The measurement of cytotoxicity by the reduction of hybridoma plaque-forming cells. J Immunol Methods. 1981;42(2):171–177. doi: 10.1016/0022-1759(81)90146-0. [DOI] [PubMed] [Google Scholar]
  17. Talmadge J. E., Meyers K. M., Prieur D. J., Starkey J. R. Role of NK cells in tumour growth and metastasis in beige mice. Nature. 1980 Apr 17;284(5757):622–624. doi: 10.1038/284622a0. [DOI] [PubMed] [Google Scholar]
  18. Warner J. F., Dennert G. Effects of a cloned cell line with NK activity on bone marrow transplants, tumour development and metastasis in vivo. Nature. 1982 Nov 4;300(5887):31–34. doi: 10.1038/300031a0. [DOI] [PubMed] [Google Scholar]

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