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[Preprint]. 2023 Mar 2:2023.03.01.23286412. [Version 1] doi: 10.1101/2023.03.01.23286412

Cerebrospinal fluid 2-hydroxyglutarate (2-HG) as a monitoring biomarker for IDH-mutant gliomas

Cecile Riviere-Cazaux, Jean M Lacey, Lucas P Carlstrom, William J Laxen, Amanda Munoz-Casabella, Matthew D Hoplin, Samar Ikram, Abdullah Bin Zubair, Katherine M Andersen, Arthur E Warrington, Paul A Decker, Timothy J Kaufmann, Jian L Campian, Jeanette E Eckel-Passow, Sani H Kizilbash, Silvia Tortorelli, Terry C Burns
PMCID: PMC10002776  PMID: 36909488

Abstract

D-2-hydroxyglutarate (D-2-HG) is a well-established oncometabolite of isocitrate dehydrogenase (IDH) mutant gliomas. While prior studies have demonstrated that D-2-HG is elevated in the cerebrospinal fluid (CSF) of patients with IDH-mutant gliomas 1,2 , no study has determined if CSF D-2-HG can provide a plausible method to evaluate therapeutic response. We are obtaining CSF samples from consenting patients during their disease course via intra-operative collection and Ommaya reservoirs. D-2-HG and D/L-2-HG consistently decreased following tumor resection and throughout chemoradiation in patients monitored longitudinally. Our early experience with this strategy demonstrates the potential for intracranial CSF D-2-HG as a monitoring biomarker for IDH-mutant gliomas.

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