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. 2023 Aug 1;150(15):dev202221. doi: 10.1242/dev.202221

Correction: The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice

Christian A Burr, Jinjing Sun, Maria V Yamburenko, Andrew Willoughby, Charles Hodgens, Samantha Louise Boeshore, Agustus Elmore, Jonathan Atkinson, Zachary L Nimchuk, Anthony Bishopp, G Eric Schaller, Joseph J Kieber
PMCID: PMC11106663  PMID: 37526652

There was an error in Development (2020) 147, dev191734 (doi:10.1242/dev.191734).

The authors have found that the cytokinin histidine kinase mutant lines used (hk5, hk6 and hk5 hk6) all contained, in addition to the stated hk5 and/or hk6 mutations, a further mutation in the HK4 gene. The mutation is a 1-bp insertion of an A residue at position 1179 in the genomic sequence (where the A in the start codon is +1):

WT HK4 sequence at CRISPR site: CGGTGGAGGATCGTGTTGCT

hk4-4 sequence at CRISPR site: CGGATGGAGGATCGTGTTGCT

The authors designated this allele hk4-4.

Once the authors discovered the problem, they sequenced all four HK genes. All versions of the lines used in the study were WT for HK3, homozygous mutant for the CRISPR-induced frameshift mutation in hk4, and had the expected hk5 and/or hk6 mutations. Therefore, the hk5 line reported in the paper is actually a hk4 hk5 double mutant, hk6 is a hk4 hk6 double mutant and hk5 hk6 is a hk4 hk5 hk6 triple mutant.

This does not substantially change the conclusions, except of course that the hk4 mutation probably also contributes to the phenotypes reported.


Articles from Development (Cambridge, England) are provided here courtesy of Company of Biologists

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