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. 1985 Feb;59(2):475–481.

The isotype and specificity of antiglobulins in BALB/c mice.

M G Jones, F C Hay
PMCID: PMC1577152  PMID: 3872191

Abstract

Antiglobulin activity was detected in the IgM class and the IgG1, IgG2a and IgG2b subclasses in normal and unstimulated BALB/c mice. There was an age related increase in their IgM antiglobulin, but this increase was not observed with IgG antiglobulin. Antiglobulin production could be induced by polyclonal activation of BALB/c mice with lipopolysaccharide, but this produces an increase in IgM antiglobulin only. IgM antiglobulin binding with mouse IgG1 and human IgG peaked on day 4 following lipopolysaccharide, whereas IgM antiglobulin binding with mouse IgG2a peaked around day 7. There was no IgM response with specificity for binding to mouse IgG2b. The level of IgG antiglobulin remained constant following polyclonal activation of BALB/c mice with lipopolysaccharide. Interestingly IgG antiglobulin binds preferentially with isologous IgG compared with heterologous IgG. Possible physiological roles have been discussed for the presence of these rheumatoid factors in normal mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dresser D. W. Most IgM-producing cells in the mouse secrete auto-antibodies (rheumatoid factor). Nature. 1978 Aug 3;274(5670):480–483. doi: 10.1038/274480a0. [DOI] [PubMed] [Google Scholar]
  3. Dresser D. W., Popham A. M. Induction of an IgM anti-(bovine)-IgG response in mice by bacterial lipopolysaccharide. Nature. 1976 Dec 9;264(5586):552–554. doi: 10.1038/264552a0. [DOI] [PubMed] [Google Scholar]
  4. Gearhart P. J., Johnson N. D., Douglas R., Hood L. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Nature. 1981 May 7;291(5810):29–34. doi: 10.1038/291029a0. [DOI] [PubMed] [Google Scholar]
  5. Grabar P. "Self"and "not-self" in immunology. Lancet. 1974 Jun 29;1(7870):1320–1322. doi: 10.1016/s0140-6736(74)90685-0. [DOI] [PubMed] [Google Scholar]
  6. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Izui S., Eisenberg R. A., Dixon F. J. IgM rheumatoid factors in mice injected with bacterial lipopolysaccharides. J Immunol. 1979 May;122(5):2096–2102. [PubMed] [Google Scholar]
  8. Klaus G. G. Generation of memory cells. III. Antibody class requirements for the generation of B-memory cells by antigen--antibody complexes. Immunology. 1979 Jun;37(2):345–351. [PMC free article] [PubMed] [Google Scholar]
  9. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  10. Nemazee D. A., Sato V. L. Enhancing antibody: a novel component of the immune response. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3828–3832. doi: 10.1073/pnas.79.12.3828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nemazee D. A., Sato V. L. Induction of rheumatoid antibodies in the mouse. Regulated production of autoantibody in the secondary humoral response. J Exp Med. 1983 Aug 1;158(2):529–545. doi: 10.1084/jem.158.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steele E. J., Cunningham A. J. High proportion of Ig-producing cells making autoantibody in normal mice. Nature. 1978 Aug 3;274(5670):483–484. doi: 10.1038/274483a0. [DOI] [PubMed] [Google Scholar]
  13. Van Snick J. L. A gene linked to the Igh-C locus controls the production of rheumatoid factor in the mouse. J Exp Med. 1981 Mar 1;153(3):738–742. doi: 10.1084/jem.153.3.738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van Snick J. L., Masson P. L. Age-dependent production of IgA and IgM autoantibodies against IgG2a in a colony of 129/Sv mice. J Exp Med. 1979 Jun 1;149(6):1519–1530. doi: 10.1084/jem.149.6.1519. [DOI] [PMC free article] [PubMed] [Google Scholar]

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