Abstract
The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment with 2-mercaptoethanol and dithiothreitol, respectively. On the other hand, dual absorptions of rabbit anti-H immune sera with killed cells of Staphylococcus aureus strain Cowan I, i.e., protein A, failed to reduce significantly H-immobilization titers of rabbit sera, although human immunoglobulins G and M were bound by protein A. It was tentatively concluded that the 2-mercaptoethanol- and dithiothreitol-refractory H-immobilizing rabbit antibodies belonged to the immunoglublin M class. H-antigen (phase) variation was not demonstrable in several extramural, clinical isolates of S. marcescens for which this phenomenon had been claimed. Rather, four of these six isolates were found to consist of cell populations of two distinct serotypes, as also borne out by bacteriocin typing; the flagellar H-antigens of the remaining two isolates were stable, with minor, hterologous H-antigen cross-reactivity.
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- Farmer J. J., 3rd Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production. Appl Microbiol. 1972 Feb;23(2):218–225. doi: 10.1128/am.23.2.218-225.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farmer J. J., 3rd Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity. Appl Microbiol. 1972 Feb;23(2):226–231. doi: 10.1128/am.23.2.226-231.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goding J. W. Use of staphylococcal protein A as an immunological reagent. J Immunol Methods. 1978;20:241–253. doi: 10.1016/0022-1759(78)90259-4. [DOI] [PubMed] [Google Scholar]
- Grimont P. A., Grimont F. Biotyping of Serratia marcescens and its use in epidemiological studies. J Clin Microbiol. 1978 Jul;8(1):73–83. doi: 10.1128/jcm.8.1.73-83.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Le Minor S., Pigache F. Etude antigénique de souches de Serratia marcescens isolées en France. I--Antigènes H: individualisation de six nouveaux facteurs H. Ann Microbiol (Paris) 1977 Aug-Sep;128(2):207–214. [PubMed] [Google Scholar]
- Le Minor S., Pigache F. Etude antigénique de souches de Serratia marcescens isolées en France. II.--Caractérisation des antigènes O et individualisation de 5 nouveaux facteurs, fréquence des sérotypes et désignation des nouveaux facteurs H. Ann Microbiol (Paris) 1978 Oct;129 B(3):407–423. [PubMed] [Google Scholar]
- Olson P. R., Weiblen B. J., O'Leary J. J., Moscowitz A. J., McCullough J. A simple technique for the inactivation of IgM antibodies using dithiothreitol. Vox Sang. 1976;30(2):149–159. doi: 10.1111/j.1423-0410.1976.tb02806.x. [DOI] [PubMed] [Google Scholar]
- Rubin S. J., Brock S., Chamberland M., Lyons R. W. Combined serotyping and biotyping of Serratia marcescens. J Clin Microbiol. 1976 Jun;3(6):582–585. doi: 10.1128/jcm.3.6.582-585.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Traub W. H. Continued surveillance of Serratia marcescens infections by bacteriocin typing: investigation of two outbreaks of cross-infection in an intensive care unit. Appl Microbiol. 1972 May;23(5):982–985. doi: 10.1128/am.23.5.982-985.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Traub W. H., Kleber I. Serotyping of Serratia marcescens: determination of serogroup (O) antigens and serological cross-reactions. Zentralbl Bakteriol Orig A. 1978 Jan;240(1):30–56. [PubMed] [Google Scholar]
- Traub W. H., Kleber I. Serotyping of Serratia marcescens: evaluation of Le Minor's H-immobilization test and description of three new flagellar H antigens. J Clin Microbiol. 1977 Feb;5(2):115–121. doi: 10.1128/jcm.5.2.115-121.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Traub W. H., Raymond E. A., Startsman T. S. Bacteriocin (Marcescin) typing of clinical isolates of Serratia marcescens. Appl Microbiol. 1971 May;21(5):837–840. doi: 10.1128/am.21.5.837-840.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]