Abstract
A method for the determination of dry matter and elemental content of individual bacterial cells is described. The method is based on energy-dispersive X-ray microanalysis in a transmission electron microscope. A theory for area correction of intensity is developed. Escherichia coli in the late exponential phase of growth and early stationary phase (glucose limited) had an average dry matter content of 278 and 154 fg/cell, respectively. Of the elements detected, sodium, magnesium, phosphorus, sulphur, chlorine, potassium, and calcium together made up 15 to 17% of the dry matter content. A phosphorus content of 4.2 to 5.4% of the dry matter was found in these cells. Volume measurements of air-dried cells gave an average of 1.20 to 1.25 micron3. These results emphasize that dry matter content and elemental composition can be measured directly on single cells from complex microbial communities.
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- Bratbak G., Dundas I. Bacterial dry matter content and biomass estimations. Appl Environ Microbiol. 1984 Oct;48(4):755–757. doi: 10.1128/aem.48.4.755-757.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chandler J. A., Battersby S. X-ray microanalysis of ultrathin frozen and freeze-dried sections of human sperm cells. J Microsc. 1976 May;107(1):55–65. doi: 10.1111/j.1365-2818.1976.tb02423.x. [DOI] [PubMed] [Google Scholar]
- Davies T. W., Morgan A. J. The application of x-ray analysis in the transmission electron analytical microscope (T.E.A.M.) to the quantitative bulk analysis of biological microsamples. J Microsc. 1976 May;107(1):47–54. doi: 10.1111/j.1365-2818.1976.tb02422.x. [DOI] [PubMed] [Google Scholar]
- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kung F. C., Raymond J., Glaser D. A. Metal ion content of Escherichia coli versus cell age. J Bacteriol. 1976 Jun;126(3):1089–1095. doi: 10.1128/jb.126.3.1089-1095.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lechene C. P., Bronner C., Kirk R. G. Electron probe microanalysis of chemical elemental content of single human red cells. J Cell Physiol. 1977 Jan;90(1):117–126. doi: 10.1002/jcp.1040900114. [DOI] [PubMed] [Google Scholar]
- Lusk J. E., Williams R. J., Kennedy E. P. Magnesium and the growth of Escherichia coli. J Biol Chem. 1968 May 25;243(10):2618–2624. [PubMed] [Google Scholar]
- Newell S. Y., Christian R. R. Frequency of dividing cells as an estimator of bacterial productivity. Appl Environ Microbiol. 1981 Jul;42(1):23–31. doi: 10.1128/aem.42.1.23-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watson S. W., Novitsky T. J., Quinby H. L., Valois F. W. Determination of bacterial number and biomass in the marine environment. Appl Environ Microbiol. 1977 Apr;33(4):940–946. doi: 10.1128/aem.33.4.940-946.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woldringh C. L., de Jong M. A., van den Berg W., Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. doi: 10.1128/jb.131.1.270-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]