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. 1985 Nov;62(2):442–448.

Enhancement of human neutrophil complement receptors: a comparison of the rosette technique with the uptake of radio-labelled anti-CR1 monoclonal antibody.

H B Richerson, G M Walsh, M J Walport, R Moqbel, A B Kay
PMCID: PMC1577443  PMID: 3936652

Abstract

We have compared the rosette technique (using C3b-coated red cells) with the uptake of a 125I F(ab')2 anti-complement receptor type 1 (CR1) monoclonal antibody (E11) for studying the phenomenon of chemotactic factor (fMLP)-induced complement receptor enhancement (CRE) on human neutrophils. With both methods the dose responses of fMLP were similar with maximal CRE being observed at 10(-7) moles 1(-1). Conversely, the time course of CRE and the effects of disodium cromoglycate (DSCG) on inhibition of enhancement were discrepant. Maximal CRE with the rosette method was observed at 30 min, whereas increased uptake of anti-CR1 had still not reached a plateau after 1 h. DSCG inhibited fMLP-induced CRE, as assessed by the rosette technique, whereas this agent had no effect on increased binding of radio-labelled anti-CR1 antibody. These results suggest that in CRE the increased adherence of C3b-coated red cells to fMLP-stimulated neutrophils, as measured by the rosette technique, is not dependent exclusively on the increased numbers of CR1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger M., O'Shea J., Cross A. S., Folks T. M., Chused T. M., Brown E. J., Frank M. M. Human neutrophils increase expression of C3bi as well as C3b receptors upon activation. J Clin Invest. 1984 Nov;74(5):1566–1571. doi: 10.1172/JCI111572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carroll M., Lukacs K., Hodson M., Kay A. B. Defective chemotactic factor-induced monocyte complement receptor enhancement in lung cancer. Clin Exp Immunol. 1983 Dec;54(3):785–792. [PMC free article] [PubMed] [Google Scholar]
  3. Craddock P. R., Hammerschmidt D., White J. G., Dalmosso A. P., Jacob H. S. Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia. J Clin Invest. 1977 Jul;60(1):260–264. doi: 10.1172/JCI108763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Durham S. R., Carroll M., Walsh G. M., Kay A. B. Leukocyte activation in allergen-induced late-phase asthmatic reactions. N Engl J Med. 1984 Nov 29;311(22):1398–1402. doi: 10.1056/NEJM198411293112202. [DOI] [PubMed] [Google Scholar]
  5. Fearon D. T., Collins L. A. Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J Immunol. 1983 Jan;130(1):370–375. [PubMed] [Google Scholar]
  6. Gallin J. I., Durocher J. R., Kaplan A. P. Interaction of leukocyte chemotactic factors with the cell surface. I. Chemotactic factor-induced changes in human granulocyte surface charge. J Clin Invest. 1975 May;55(5):967–974. doi: 10.1172/JCI108026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hogg N., Ross G. D., Jones D. B., Slusarenko M., Walport M. J., Lachmann P. J. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236–243. doi: 10.1002/eji.1830140307. [DOI] [PubMed] [Google Scholar]
  8. Iida K., Mornaghi R., Nussenzweig V. Complement receptor (CR1) deficiency in erythrocytes from patients with systemic lupus erythematosus. J Exp Med. 1982 May 1;155(5):1427–1438. doi: 10.1084/jem.155.5.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kay A. B., Glass E. J., Salter D. M. Leucoattractants enhance complement receptors on human phagocytic cells. Clin Exp Immunol. 1979 Nov;38(2):294–299. [PMC free article] [PubMed] [Google Scholar]
  10. Kay A. B., Walsh G. M. Chemotactic factor-induced enhancement of the binding of human immunoglobulin classes and subclasses to neutrophils and eosinophils. Clin Exp Immunol. 1984 Sep;57(3):729–734. [PMC free article] [PubMed] [Google Scholar]
  11. Papageorgiou N., Carroll M., Durham S. R., Lee T. H., Walsh G. M., Kay A. B. Complement receptor enhancement as evidence of neutrophil activation after exercise-induced asthma. Lancet. 1983 Nov 26;2(8361):1220–1223. doi: 10.1016/s0140-6736(83)91270-9. [DOI] [PubMed] [Google Scholar]
  12. Sklar L. A., Oades Z. G., Finney D. A. Neutrophil degranulation detected by right angle light scattering: spectroscopic methods suitable for simultaneous analyses of degranulation or shape change, elastase release, and cell aggregation. J Immunol. 1984 Sep;133(3):1483–1487. [PubMed] [Google Scholar]

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