Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Aug 1;229(3):771–778. doi: 10.1042/bj2290771

Major changes in phosphorylation of chromatin-associated non-histone proteins accompany development in the slime mould Dictyostelium discoideum.

J H Sinclair, D Rickwood
PMCID: PMC1145123  PMID: 4052024

Abstract

Striking changes in protein and RNA synthesis were shown to accompany development in the slime mould Dictyostelium discoideum. These changes are, at least in part, directly attributable to control at the level of transcription. Analysis of nuclear proteins and their states of phosphorylation by two-dimensional gel electrophoresis and autoradiography showed only minor changes in the species of proteins detectable by Coomassie Blue staining during the vegetative growth and development phases. Major changes, however, were detected in their patterns of phosphorylation, with major differences from the vegetative pattern occurring during both early development (0-2h) and late development (8-12h). These changes coincide with major changes in polypeptide synthesis and in nuclear and cytoplasmic RNA complexity.

Full text

PDF
771

Images in this article

774Fig. 1.
on p.774
774Fig. 2.
on p.774
775Fig. 3.
on p.775
776Fig. 4.
on p.776
776Fig. 5.
on p.776
777Fig. 6.
on p.777

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamietz P., Hilz H. Poly(adenosine diphosphate ribose) is covalently linked to nuclear proteins by two types of bonds. Hoppe Seylers Z Physiol Chem. 1976 Apr;357(4):527–534. doi: 10.1515/bchm2.1976.357.1.527. [DOI] [PubMed] [Google Scholar]
  2. Alton T. H., Lodish H. F. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. doi: 10.1016/0012-1606(77)90118-x. [DOI] [PubMed] [Google Scholar]
  3. Alton T. H., Lodish H. F. Synthesis of developmentally regulated proteins in Dictyostelium discoideum which are dependent on continued cell-cell interaction. Dev Biol. 1977 Oct 1;60(1):207–216. doi: 10.1016/0012-1606(77)90119-1. [DOI] [PubMed] [Google Scholar]
  4. Batts-Young B., Lodish H. F., Jacobson A. Similarity of the primary sequences of ribosomal RNAs isolated from vegetative and developing cells of Dictyostelium discoidem. Dev Biol. 1980 Aug;78(2):352–364. doi: 10.1016/0012-1606(80)90340-1. [DOI] [PubMed] [Google Scholar]
  5. Blumberg D. D., Lodish H. F. Changes in the messenger RNA population during differentiation of dictyostelium discoideum. Dev Biol. 1980 Aug;78(2):285–300. doi: 10.1016/0012-1606(80)90337-1. [DOI] [PubMed] [Google Scholar]
  6. Blumberg D. D., Lodish H. F. Complexity of nuclear and polysomal RNAs in growing Dictyostelium discoideum cells. Dev Biol. 1980 Aug;78(2):268–284. doi: 10.1016/0012-1606(80)90336-x. [DOI] [PubMed] [Google Scholar]
  7. Cocucci S. M., Sussman M. RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas. J Cell Biol. 1970 May;45(2):399–407. doi: 10.1083/jcb.45.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Comings D. E., Tack L. O. Non-histone proteins. The effect of nuclear washes and comparison of metaphase and interphase chromatin. Exp Cell Res. 1973 Nov;82(1):175–191. doi: 10.1016/0014-4827(73)90260-7. [DOI] [PubMed] [Google Scholar]
  9. Delaney S. J., Wilkinson D. G., Hames B. D. Phosphorylation of spore coat proteins during development of Dictyostelium discoideum. Biochem J. 1983 Jun 15;212(3):699–703. doi: 10.1042/bj2120699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Firtel R. A. Changes in the expression of single-copy DNA during development of the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1972 May 28;66(3):363–377. doi: 10.1016/0022-2836(72)90420-2. [DOI] [PubMed] [Google Scholar]
  11. Firtel R. A., Pederson T. Ribonucleoprotein particles containing heterogeneous nuclear RNA in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Jan;72(1):301–305. doi: 10.1073/pnas.72.1.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gezelius K., Felter S., Stahl A. Etude des polyphosphates pendant le développement multicellulaire de dictyostelium discoideum. C R Acad Sci Hebd Seances Acad Sci D. 1973 Jan 3;276(1):117–119. [PubMed] [Google Scholar]
  13. Kuehn G. D., Affolter H. U., Atmar V. J., Seebeck T., Gubler U., Braun R. Polyamine-mediated phosphorylation of a nucleolar protein from Physarum polycephalum that stimulates rRNA synthesis. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2541–2545. doi: 10.1073/pnas.76.6.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Landfear S. M., Lefebvre P., Chung S., Lodish H. F. Transcriptional control of gene expression during development of Dictyostelium discoideum. Mol Cell Biol. 1982 Nov;2(11):1417–1426. doi: 10.1128/mcb.2.11.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  16. Pederson T. Proteins associated with heterogeneous nuclear RNA in eukaryotic cells. J Mol Biol. 1974 Feb 25;83(2):163–183. doi: 10.1016/0022-2836(74)90386-6. [DOI] [PubMed] [Google Scholar]
  17. Ramagopal S., Ennis H. L. Regulation of synthesis of cell-specific ribosomal proteins during differentiation of Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1981 May;78(5):3083–3087. doi: 10.1073/pnas.78.5.3083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rickwood D., MacGillivray A. J. Improved techniques for the fractionation of non-histone proteins of chromatin on hydroxyapatite. Eur J Biochem. 1975 Feb 21;51(2):593–601. doi: 10.1111/j.1432-1033.1975.tb03961.x. [DOI] [PubMed] [Google Scholar]
  19. Rickwood D., Osman M. S. Characterisation of poly(ADP-Rib) polymerase activity in nuclei from the slime mould Dictyostelium discoideum. Mol Cell Biochem. 1979 Oct 15;27(2):79–84. doi: 10.1007/BF00218351. [DOI] [PubMed] [Google Scholar]
  20. Samarina O. P., Lukanidin E. M., Molnar J., Georgiev G. P. Structural organization of nuclear complexes containing DNA-like RNA. J Mol Biol. 1968 Apr 14;33(1):251–263. doi: 10.1016/0022-2836(68)90292-1. [DOI] [PubMed] [Google Scholar]
  21. Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
  22. Tsai S. Y., Harris S. E., Tsai M. J., O'Malley B. W. Effects of estrogen on gene expression in chick oviduct. The role of chromatin proteins in regulating transcription of the ovalbumin gene. J Biol Chem. 1976 Aug 10;251(15):4713–4721. [PubMed] [Google Scholar]
  23. Tuchman J., Alton T., Lodish H. F. Preferential synthesis of actin during early development of the slime mold Dictyostelium discoideum. Dev Biol. 1974 Sep;40(1):116–128. doi: 10.1016/0012-1606(74)90113-4. [DOI] [PubMed] [Google Scholar]
  24. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weisbrod S., Weintraub H. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630–634. doi: 10.1073/pnas.76.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Williams J. G., Lloyd M. M., Devine J. M. Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideum. Cell. 1979 Aug;17(4):903–913. doi: 10.1016/0092-8674(79)90330-1. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES