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. 1977 Apr 15;164(1):161–167. doi: 10.1042/bj1640161

Effects of adenine nucleotides and phosphate on adenosine triphosphate sulphurylase from Anabaena cylindrica.

S K Sawhney, D J Nicholas
PMCID: PMC1164770  PMID: 406902

Abstract

Production of adenosine 5'-[35S]sulphatophosphate by a partially purified ATP sulphurylase from Anabaena cylindrica was inhibited by AMP, ADP and P1. Decreases in enzyme activity in the presence of these inhibitors were reversed by increasing the concentrations of ATP. The adenine nucleotides inhibited the enzyme competitively with respect to ATP. In the presence of P1, ATP showed a positive co-operative effect on enzyme activity. The inhibition by P1 was enhanced by increasing concentrations of MG2+. The effects of the adenine nucleotides and the interaction of P1 and Mg2+ on ATP sulphurylase activity are discussed in relation to the regulation of sulphate assimilation via the energy metabolism of the alga.

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Selected References

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  1. Adams C. A., Warnes G. M., Nicholas D. J. Preparation of labeled adenosine 5'-phosphosulfate using APS reductase from Thibacillus denitrificans. Anal Biochem. 1971 Jul;42(1):207–213. doi: 10.1016/0003-2697(71)90028-5. [DOI] [PubMed] [Google Scholar]
  2. Balharry G. J., Nicholas D. J. New assay for ATP-sulphurylase using the luciferin-luciferase method. Anal Biochem. 1971 Mar;40(1):1–17. doi: 10.1016/0003-2697(71)90078-9. [DOI] [PubMed] [Google Scholar]
  3. Blair J. M. Magnesium, potassium, and the adenylate kinase equilibrium. Magnesium as a feedback signal from the adenine nucleotide pool. Eur J Biochem. 1970 Apr;13(2):384–390. doi: 10.1111/j.1432-1033.1970.tb00940.x. [DOI] [PubMed] [Google Scholar]
  4. Brownell P. F., Nicholas D. J. Some Effects of Sodium on Nitrate Assimilation and N(2) Fixation in Anabaena cylindrica. Plant Physiol. 1967 Jul;42(7):915–921. doi: 10.1104/pp.42.7.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DEVITO P. C., DREYFUSS J. METABOLIC REGULATION OF ADENOSINE TRIPHOSPHATE SULFURYLASE IN YEAST. J Bacteriol. 1964 Nov;88:1341–1348. doi: 10.1128/jb.88.5.1341-1348.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dreyfuss J., Pardee A. B. Regulation of sulfate transport in Salmonella typhimurium. J Bacteriol. 1966 Jun;91(6):2275–2280. doi: 10.1128/jb.91.6.2275-2280.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hawes C. S., Nicholas D. J. Adenosine 5'-triphosphate sulphurylase from Saccharomyces cerevisiae. Biochem J. 1973 Jul;133(3):541–550. doi: 10.1042/bj1330541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hodson R. C., Schiff J. A. Preparation of adenosine-3'-phosphate-51-phosphosulfate (PAPS): an improved enzymatic method using Chlorella pyrenoidosa. Arch Biochem Biophys. 1969 Jun;132(1):151–156. doi: 10.1016/0003-9861(69)90347-6. [DOI] [PubMed] [Google Scholar]
  9. Hodson R. C., Schiff J. A., Scarsella A. J., Levinthal M. Studies of Sulfate Utilization by Algae. 6. Adenosine-3'-Phosphate-5'-Phosphosulfate (PAPS) as an Intermediate in Thiosulfate Formation From Sulfate by Cell-Free Extracts of Chlorella. Plant Physiol. 1968 Apr;43(4):563–569. doi: 10.1104/pp.43.4.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kitchin S. E., Watts D. C. Comparison of adenylate kinase from liver and muscle of normal mice and those with an hereditary muscular dystrophy. Biochim Biophys Acta. 1974 Oct 17;364(2):272–283. doi: 10.1016/0005-2744(74)90013-8. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Levi A. S., Wolf G. Purification and properties of the enzyme ATP-sulfurylase and its relation to vitamin A. Biochim Biophys Acta. 1969 Apr 22;178(2):262–282. doi: 10.1016/0005-2744(69)90395-7. [DOI] [PubMed] [Google Scholar]
  13. Lik-Shing Tsang M., Schiff J. A. Properties of enzyme fraction A from Chlorella and copurification of 3' (2'), 5'-biphosphonucleoside 3' (2')-phosphohydrolase, adenosine 5'phosphosulfate sulfohydrolase and adenosine-5'-phosphosulfate cyclase activities. Eur J Biochem. 1976 May 17;65(1):113–121. doi: 10.1111/j.1432-1033.1976.tb10395.x. [DOI] [PubMed] [Google Scholar]
  14. Onajobi F. D. Effects of adenine nucleotides on rice-root adenosine triphosphate sulphurylase activity in vitro. Biochem J. 1975 Jul;149(1):301–304. doi: 10.1042/bj1490301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schmidt A. Sulfate reduction in a cell-free system of Chlorella. The ferredoxin dependent reduction of a protein-bound intermediate by a thiosulfonate reductase. Arch Mikrobiol. 1973 Oct 4;93(1):29–52. [PubMed] [Google Scholar]
  16. Tweedie J. W., Segel I. H. Adenosine triphosphate sulfurylase from Penicillium chrysogenum. II. Physical, kinetic, and regulatory properties. J Biol Chem. 1971 Apr 25;246(8):2438–2446. [PubMed] [Google Scholar]
  17. Wheldrake J. F. Intracellular concentration of cysteine in Escherichia coli and its relation to repression of the sulphate-activating enzymes. Biochem J. 1967 Nov;105(2):697–699. doi: 10.1042/bj1050697. [DOI] [PMC free article] [PubMed] [Google Scholar]

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