Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1974 Jul;27(1):43–52.

In vitro antibody response to a distinct antigenic determinant of Escherichia coli β-D-galactosidase

Effect of antigen dosage and co-cultivation of lymph node fragments on peak titres and duration of antibody synthesis

A J L Macario, E Conway De Macario
PMCID: PMC1445580  PMID: 4136807

Abstract

The anti-β-D-galactosidase activating antibody response in lymph node fragment cultures was investigated in an attempt to understand the mechanism determining the magnitude of an immune response towards a natural hapten, which occurs only once in each antigen molecule, under in vitro conditions that preserve the histology of the responding tissue. Fragments were individually challenged for defined time periods, and washed; cultures with one and two fragments were then set up. Co-cultivation of fragments produced titres higher than predicted from results in single fragment cultures. This happened even when the latter cultures produced low titres because of excessive antigen dosages. Co-cultivation of fragments also improved the duration of the antibody response. These effects appeared to correlate with the number of memory cells present in the fragments at the beginning of the cultures. These observations, together with data obtained by manipulating different doses and times of antigen exposure suggest that collaboration between cells and determinants with different specificities may occur, not only in the initial induction, but also in the sequential stimulation of antibody-forming clones. It is postulated that the co-operative mechanism shown to act in the initiation of the antibody response by cell suspensions toward polyvalent artificial conjugates may also operate when intact lymphoid tissue is reached by a single bacterial determinant in its natural molecular configuration

Full text

PDF
43

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Byers V. S., Sercarz E. E. Induction and reversal of immune paralysis in vitro. J Exp Med. 1970 Nov;132(5):845–857. doi: 10.1084/jem.132.5.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Celada F., Ellis J., Bodlund K., Rotman B. Antibody-mediated activation of a defective beta-D-galactosidase. II. Immunological relationship between the normal and the defective enzyme. J Exp Med. 1971 Sep 1;134(3 Pt 1):751–764. doi: 10.1084/jem.134.3.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Celada F. Quantitative studies of the adoptive immunological memory in mice. II. Linear transmission of cellular memory. J Exp Med. 1967 Feb 1;125(2):199–211. doi: 10.1084/jem.125.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doria G., Agarossi G., Di Pietro S. Enhancing activity of thymocyte culture cell-free medium on the in vitro immune response of spleen cells from neonatally thymectomized mice to sheep RBC. J Immunol. 1972 Jan;108(1):268–270. [PubMed] [Google Scholar]
  5. Feldmann M., Basten A. Cell interactions in the immune response in vitro. I. Metabolic activities of T cells in a collaborative antibody response. Eur J Immunol. 1972 Jun;2(3):213–224. doi: 10.1002/eji.1830020305. [DOI] [PubMed] [Google Scholar]
  6. Macario A. J., Conway de Macario E., Celada F. An immunoenzymatic system to study in vitro immune responses. Synthesis of antibodies directed toward different -D-galactosidase determinants in lymph node fragment cultures. Immunology. 1973 Feb;24(2):237–248. [PMC free article] [PubMed] [Google Scholar]
  7. Macario A. J., Conway de Macario E., Franceschi C., Celada F. Maturation of the immkune response in vitro. Focal fluctuation and changes in affinity of anti-beta-D-galactosidase activating antibody. J Exp Med. 1972 Aug 1;136(2):353–368. doi: 10.1084/jem.136.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rotman M. B., Celada F. Antibody-mediated activation of a defective beta-D-galactosidase extracted from an Escherichia coli mutant. Proc Natl Acad Sci U S A. 1968 Jun;60(2):660–667. doi: 10.1073/pnas.60.2.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Unanue E. R., Askonas B. A. Persistence of immunogenicity of antigen after uptake by macrophages. J Exp Med. 1968 May 1;127(5):915–926. doi: 10.1084/jem.127.5.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Yuan L., Campbell D. H. The in vivo and in vitro immunogenicity and antigenic specificity of lymphoid antigen fragment-oligoribonucleopeptide conjugates. Immunochemistry. 1972 Jan;9(1):1–8. doi: 10.1016/0019-2791(72)90277-7. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES