Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1973 Aug;25(2):227–235.

Cellular immunity in experimental glomerulonephritis of rats

II. A sequential study of immunological events occurring during development of the disease*

A Litwin, L E Adams, Y Yamauchi, E V Hess
PMCID: PMC1422862  PMID: 4200317

Abstract

The exact roles played by specific antibody, circulating antigen—antibody complexes and cellular immunity in the causation of experimental glomerulonephritis of rats (EGN) need clarification and a sequential study of these factors was therefore undertaken. Rats immunized with renal tubular antigen and studied for humoral response only (group I) produced neither precipitating nor 2- or 48-hour PCA antibodies but did have an antibody titre by an immunofluorescent test against the proximal portion of the proximal convoluted tubular cells by the second to sixth week post-immunization in six of six animals. A C1q test for antigen—antibody complexes became positive in most rats at the time of onset of proteinuria. Rats in group II were studied for cellular immunity to the renal tubular antigen. Skin tests did not become positive until approximately the time of onset of proteinuria when peripheral blood lymphocyte cultures were also stimulated in seven of nine rats tested. Direct immunofluorescence of the kidneys from this group revealed a linear deposition of γG and renal tubular antigen along the glomerular basement membrane. Since both cellular immunity and antigen—antibody complexes involving the renal tubular antigen were present at the onset of proteinuria, both modalities may play a significant role in EGN.

Full text

PDF
227

Images in this article

232FIG. 1
on p.232
234FIG. 2
on p.234

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agnello V., Winchester R. J., Kunkel H. G. Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology. 1970 Dec;19(6):909–919. [PMC free article] [PubMed] [Google Scholar]
  2. Edgington T. S., Glassock R. J., Dixon F. J. Autologous immune complex nephritis induced with renal tubular antigen. I. Identification and isolation of the pathogenetic antigen. J Exp Med. 1968 Mar 1;127(3):555–572. doi: 10.1084/jem.127.3.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edgington T. S., Glassock R. J., Dixon F. J. Autologous immune-complex pathogenesis of experimental allergic glomerulonephritis. Science. 1967 Mar 17;155(3768):1432–1434. doi: 10.1126/science.155.3768.1432. [DOI] [PubMed] [Google Scholar]
  4. HESS E. V., ASHWORTH C. T., ZIFF M. Transfer of an autoimmune nephrosis in the rat by means of lymph node cells. J Exp Med. 1962 Feb 1;115:421–438. doi: 10.1084/jem.115.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HEYMANN W., HACKEL D. B., HARWOOD S., WILSON S. G., HUNTER J. L. Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med. 1959 Apr;100(4):660–664. doi: 10.3181/00379727-100-24736. [DOI] [PubMed] [Google Scholar]
  6. Litwin A., Adams L. E., Levy R., Cline S., Hess E. V. Cellular immunity in experimental glomerulonephritis of rats. I. Delayed hypersensitivity and lymphocyte stimulation studies with renal tubular antigens. Immunology. 1971 May;20(5):755–766. [PMC free article] [PubMed] [Google Scholar]
  7. Stechschulte D. J., Austen K. F., Bloch K. J. Antibodies involved in antigen-induced release of slow reacting substance of anaphylaxis (SRS-A) in the guinea pig and rat. J Exp Med. 1967 Jan 1;125(1):127–147. doi: 10.1084/jem.125.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Temple A., Loewi G. Transfer of delayed hypersensitivity in rats. Immunology. 1971 May;20(5):799–802. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES