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. 1969 Nov;64(3):973–980. doi: 10.1073/pnas.64.3.973

A STIMULATION BY CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE OF AMINO ACID ACTIVATION AND POLYMERIZATION IN RETICULOCYTE HEMOLYSATES*

Martin Malkin 1,†, Fritz Lipmann 1
PMCID: PMC223331  PMID: 4313337

Abstract

With reticulocyte supernatant, cyclic 3′,5′-adenosine monophosphate at concentrations of 10-3 to 10-2M causes stimulation of aminoacyl-tRNA synthetases for some, e.g., valine and leucine, but not all, amino acids; it is highest at nonsaturating concentrations of ATP. Similar concentrations of cyclic 3′,5′-adenosine monophosphate are found to stimulate phenylalanine polymerization from phenylalanyl transfer ribonucleic acid on polyuridylic acid-charged reticulocyte ribosomes. The degree of stimulation is highest at low GTP concentrations. It is abolished by addition of phosphoenolpyruvate + pyruvate kinase, which stimulate similarly or more effectively at low GTP levels. Under the conditions of these experiments, cyclic 3′,5′-adenosine monophosphate did not appreciably inhibit GTP hydrolysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CONWAY T. W., LIPMANN F. CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS. Proc Natl Acad Sci U S A. 1964 Dec;52:1462–1469. doi: 10.1073/pnas.52.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FERGUSON J. J., Jr PROTEIN SYNTHESIS AND ADRENOCORTICOTROPIN RESPONSIVENESS. J Biol Chem. 1963 Aug;238:2754–2759. [PubMed] [Google Scholar]
  3. FUCHS F., BRIGGS F. N. Direct isolation of a soluble relaxing system from muscle. Biochim Biophys Acta. 1961 Aug 5;51:423–425. doi: 10.1016/0006-3002(61)90200-1. [DOI] [PubMed] [Google Scholar]
  4. Felicetti L., Lipmann F. Comparison of amino acid polymerization factors isolated from rat liver and rabbit reticulocytes. Arch Biochem Biophys. 1968 May;125(2):548–557. doi: 10.1016/0003-9861(68)90613-9. [DOI] [PubMed] [Google Scholar]
  5. GRUNBERG-MANAGO M., ORITZ P. J., OCHOA S. Enzymatic synthesis of nucleic acidlike polynucleotides. Science. 1955 Nov 11;122(3176):907–910. doi: 10.1126/science.122.3176.907. [DOI] [PubMed] [Google Scholar]
  6. Gill G. N., Garren L. D. On the mechanism of action of adrenocorticotropic hormone: the binding of cyclic-3',5'-adenosine monophosphate to an adrenal cortical protein. Proc Natl Acad Sci U S A. 1969 Jun;63(2):512–519. doi: 10.1073/pnas.63.2.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grahame-Smith D. G., Butcher R. W., Ney R. L., Sutherland E. W. Adenosine 3',5'-monophosphate as the intracellular mediator of the action of adrenocorticotropic hormone on the adrenal cortex. J Biol Chem. 1967 Dec 10;242(23):5535–5541. [PubMed] [Google Scholar]
  8. Khairallah E. A., Pitot H. C. 3',5'-Cyclic AMP and the release of polysome-bound proteins in vitro. Biochem Biophys Res Commun. 1967 Nov 17;29(3):269–273. doi: 10.1016/0006-291x(67)90447-0. [DOI] [PubMed] [Google Scholar]
  9. Lissitzky S., Manté S., Attali J. C., Cartouzou G. Action of 3',5'-cyclic adenosinemonophosphate on the protein synthesizing capacity of thyroid polyribosomes in vitro. Biochem Biophys Res Commun. 1969 May 22;35(4):437–443. doi: 10.1016/0006-291x(69)90364-7. [DOI] [PubMed] [Google Scholar]
  10. Malkin M., Lipmann F. Fusidic acid: inhibition of factor T2 in reticulocyte protein synthesis. Science. 1969 Apr 4;164(3875):71–72. doi: 10.1126/science.164.3875.71. [DOI] [PubMed] [Google Scholar]
  11. Mitra K., Mehler A. H. The role of transfer ribonucleic acid in the pyrophsphate exchange reaction of arginine-transfer ribonucleic acid synthetase. J Biol Chem. 1966 Nov 10;241(21):5161–5162. [PubMed] [Google Scholar]
  12. NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
  13. Pastan I., Perlman R. L. Stimulation of tryptophanase synthesis in Escherichia coli by cyclic 3',5'-adenosine monophosphate. J Biol Chem. 1969 Apr 25;244(8):2226–2232. [PubMed] [Google Scholar]
  14. Perlman R. L., Pastan I. Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate. J Biol Chem. 1968 Oct 25;243(20):5420–5427. [PubMed] [Google Scholar]
  15. Tao M., Lipmann F. Isolation of adenyl cyclase from Escherichia coli. Proc Natl Acad Sci U S A. 1969 May;63(1):86–92. doi: 10.1073/pnas.63.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taunton O. D., Roth J., Pastan I. ACTH stimulation of adenyl cyclase in adrenal homogenates. Biochem Biophys Res Commun. 1967 Oct 11;29(1):1–7. doi: 10.1016/0006-291x(67)90531-1. [DOI] [PubMed] [Google Scholar]
  17. Ullmann A., Monod J. Cyclic AMP as an antagonist of catabolite repression in Escherichia coli. FEBS Lett. 1968 Nov;2(1):57–60. doi: 10.1016/0014-5793(68)80100-0. [DOI] [PubMed] [Google Scholar]
  18. WETTSTEIN F. O., STAEHELIN T., NOLL H. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome. Nature. 1963 Feb 2;197:430–435. doi: 10.1038/197430a0. [DOI] [PubMed] [Google Scholar]

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