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. 1974 Jun;71(6):2440–2444. doi: 10.1073/pnas.71.6.2440

Effect of Poliovirus Double-Stranded RNA on Viral and Host-Cell Protein Synthesis

M L Celma 1, E Ehrenfeld 1
PMCID: PMC388473  PMID: 4366768

Abstract

Cell-free protein-synthesizing systems that initiate on endogenous messenger RNA have been developed from uninfected and poliovirus-infected HeLa cells. Poliovirus double-stranded RNA is an effective inhibitor of protein synthesis in these extracts, and both cell-directed and virus-specific protein synthesis are equally sensitive to the inhibitory action of double-stranded RNA. The concentrations of double-stranded RNA required for inhibition are not achieved in the infected cell at early times after infection when host-cell shut-off occurs, but rather are achieved only late in infection when virus-specific protein synthesis begins to decline. This indicates that double-stranded RNA does not act as a direct agent to inhibit host cell protein synthesis following infection by poliovirus. The possible significance of inhibition by double-stranded RNA of poliovirus-specific protein synthesis is discussed.

Keywords: in vitro protein synthesis, host-cell “shut-off”

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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