Skip to main content
NCBI home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1979 Jun;60(3):335–339.

The effect of a calcium antagonist (D600) on isoprenaline-induced myocardial necrosis in the rat.

E Steen, N Woolf, T Powell, P M Rowles
PMCID: PMC2041454  PMID: 475980

Abstract

The effect of inhibiting isoprenaline-induced intracellular calcium accumulation on the degree of damage produced in the rat myocardium by this amine has been investigated by simultaneously dosing rats with the calcium antagonistic drug D600. The degree of myocardial necrosis produced in animals given isoprenaline alone and those given D600 was measured by the use of a standard point counting method to show absence of formazan from dead muscle fibres in sections treated to demonstrate succinic dehydrogenase. The use of the calcium antagonistic drug D600 considerably reduced the degree of myocardial damage produced by a standard dose of isoprenaline bitartrate. This was associated with a decrease in the isoprenaline-induced cellular calcium uptake. The results are discussed in relation to the possible protection conferred by lowering of the calcium influx at the cell membrane and the maintenance of the cells' high-energy phosphates at a level which permits "normal" cell function.

Full text

PDF
335

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom S., Cancilla P. A. Myocytolysis and mitochondrial calcification in rat myocardium after low doses of isoproterenol. Am J Pathol. 1969 Mar;54(3):373–391. [PMC free article] [PubMed] [Google Scholar]
  2. Bloom S., Davis D. L. Calcium as mediator of isoproterenol-induced myocardial necrosis. Am J Pathol. 1972 Dec;69(3):459–470. [PMC free article] [PubMed] [Google Scholar]
  3. CHAPPEL C. I., RONA G., BALAZS T., GAUDRY R. Severe myocardial necrosis produced by isoproterenol in the rat. Arch Int Pharmacodyn Ther. 1959 Oct 1;122:123–128. [PubMed] [Google Scholar]
  4. Ceremuzyński L., Staszewska-Barczak J., Herbaczynska-Cedro K. Cardiac rhythm disturbances and the release of catecholamines after acute coronary occlusion in dogs. Cardiovasc Res. 1969 Apr;3(2):190–197. doi: 10.1093/cvr/3.2.190. [DOI] [PubMed] [Google Scholar]
  5. Csapó Z., Dusek J., Rona G. Early alterations of the cardiac muscle cells in isoproterenol-induced necrosis. Arch Pathol. 1972 Apr;93(4):356–365. [PubMed] [Google Scholar]
  6. Dhalla N. S. Involvement of membrane systems in heart failure due to intracellular calcium overload and deficiency. J Mol Cell Cardiol. 1976 Sep;08(9):661–667. doi: 10.1016/0022-2828(76)90008-0. [DOI] [PubMed] [Google Scholar]
  7. Hoffman J. I., Buckberg G. D. Pathophysiology of subendocardial ischaemia. Br Med J. 1975 Jan 11;1(5949):76–79. doi: 10.1136/bmj.1.5949.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katz A. M. Contractile proteins of the heart. Physiol Rev. 1970 Jan;50(1):63–158. doi: 10.1152/physrev.1970.50.1.63. [DOI] [PubMed] [Google Scholar]
  9. Kjekshus J. K., Mjos O. D. Effect of free fatty acids on myocardial function and metabolism in the ischemic dog heart. J Clin Invest. 1972 Jul;51(7):1767–1776. doi: 10.1172/JCI106978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langer G. A. Ion fluxes in cardiac excitation and contraction and their relation to myocardial contractility. Physiol Rev. 1968 Oct;48(4):708–757. doi: 10.1152/physrev.1968.48.4.708. [DOI] [PubMed] [Google Scholar]
  11. Lefkowitz R. J., Limbird L. E., Mukherjee C., Caron M. G. The beta-adrenergic receptor and adenylate cyclase. Biochim Biophys Acta. 1976 Apr 13;457(1):1–39. doi: 10.1016/0304-4157(76)90012-5. [DOI] [PubMed] [Google Scholar]
  12. Lehninger A. L., Carafoli E., Rossi C. S. Energy-linked ion movements in mitochondrial systems. Adv Enzymol Relat Areas Mol Biol. 1967;29:259–320. doi: 10.1002/9780470122747.ch6. [DOI] [PubMed] [Google Scholar]
  13. Nayler W. G., Grau A., Slade A. A protective effect of verapamil on hypoxic heart muscle. Cardiovasc Res. 1976 Nov;10(6):650–662. doi: 10.1093/cvr/10.6.650. [DOI] [PubMed] [Google Scholar]
  14. Opie L. H. Effect of fatty acids on contractility and rhythm of the heart. Nature. 1970 Sep 5;227(5262):1055–1056. doi: 10.1038/2271055a0. [DOI] [PubMed] [Google Scholar]
  15. Otto D. A., Ontko J. A. Activation of mitochondrial fatty acid oxidation by calcium. Conversion to the energized state. J Biol Chem. 1978 Feb 10;253(3):789–799. [PubMed] [Google Scholar]
  16. Reuter H. Localization of beta adrenergic receptors, and effects of noradrenaline and cyclic nucleotides on action potentials, ionic currents and tension in mammalian cardiac muscle. J Physiol. 1974 Oct;242(2):429–451. doi: 10.1113/jphysiol.1974.sp010716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woolf N., Davies M. J., Shaw M. J., Trickey R. J. Exeriences with isoprenaline induced myocardial necrosis in the rat. J Pathol. 1976 Oct;120(2):65–73. doi: 10.1002/path.1711200202. [DOI] [PubMed] [Google Scholar]
  18. Woolf N., Steen E., Rosen D., Trickey R., Long P., Miller A. L., Rowles P. M. The effect of anti-lipolytic agents on isoprenaline-induced myocardial necrosis in the rat. Br J Exp Pathol. 1977 Aug;58(4):438–443. [PMC free article] [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES