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. 1974 Feb;138(2):233–237. doi: 10.1042/bj1380233

Sterol biosynthesis in the free-living nematode Panagrellus redivivus

James D Willett 1, William L Downey 1
PMCID: PMC1166200  PMID: 4822732

Abstract

The fate of the known sterol precursor squalene 2,3-oxide was investigated in the free-living nematode Panagrellus redivivus. The nematodes were cultured axenically in the presence of [4-3H]squalene 2,3-oxide. Radioactivity was found in the total lipids of the isolated nematodes. Essentially all of the radioactivity encountered in the total lipids was found in the non-saponifiable fraction. The components present in the non-saponifiable fraction were separated and isolated by t.l.c. Three labelled components were identified by a combination of t.l.c., g.l.c. and mass spectroscopy. It is established that P. redivivus has the capacity for biosynthesis of lanosterol. No labelled C27 sterols could be detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolla R. I., Weinstein P. P., Lou C. In vitro nutritional requirements of Nippostrongylus brasiliensis. I. Effects of sterols, sterol derivatives and heme compounds on the free-living stages. Comp Biochem Physiol B. 1972 Nov 15;43(3):487–501. doi: 10.1016/0305-0491(72)90132-0. [DOI] [PubMed] [Google Scholar]
  2. CLAYTON R. B., NELSON A. N., FRANTZ I. D., Jr THE SKIN STEROLS OF NORMAL AND TRIPARANOL-TREATED RATS. J Lipid Res. 1963 Apr;4:166–176. [PubMed] [Google Scholar]
  3. CLAYTON R. B. THE UTILIZATION OF STEROLS BY INSECTS. J Lipid Res. 1964 Jan;5:3–19. [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. HASLEWOOD G. A. Steroids in marine organisms. Ann N Y Acad Sci. 1960 Nov 17;90:877–883. doi: 10.1111/j.1749-6632.1960.tb26430.x. [DOI] [PubMed] [Google Scholar]
  6. Hieb W. F., Rothstein M. Sterol requirement for reproduction of a free-living nematode. Science. 1968 May 17;160(3829):778–780. doi: 10.1126/science.160.3829.778. [DOI] [PubMed] [Google Scholar]
  7. Hieb W. F., Stokstad E. L., Rothstein M. Heme requirement for reproduction of a free-living nematode. Science. 1970 Apr 3;168(3927):143–144. doi: 10.1126/science.168.3927.143. [DOI] [PubMed] [Google Scholar]
  8. Lehoux J. G., Sandor T. The occurrence of steroids and steroid metabolizing enzyme systems in invertebrates. A review. Steroids. 1970 Aug;16(2):141–171. doi: 10.1016/s0039-128x(70)80102-7. [DOI] [PubMed] [Google Scholar]
  9. Nes W. R. Regulation of the sequencing in sterol biosynthesis. Lipids. 1971 Apr;6(4):219–224. doi: 10.1007/BF02538391. [DOI] [PubMed] [Google Scholar]
  10. Rothstein M. Nematode biochemistry. IX. Lack of sterol biosynthesis in free-living nematodes. Comp Biochem Physiol. 1968 Oct;27(1):309–317. doi: 10.1016/0010-406x(68)90773-1. [DOI] [PubMed] [Google Scholar]
  11. Willett J. D., Downey W. L. Isolation and identification of lanosterol as a normal constituent of the lipids of the free living nematode Panagrellus redivivus. Comp Biochem Physiol B. 1973 Sep 15;46(1):139–142. doi: 10.1016/0305-0491(73)90054-0. [DOI] [PubMed] [Google Scholar]

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