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. 1971 Feb;20(2):191–203.

Antibody production in mice

II. The mechanism of antigenic stimulation in the secondary immune response

T Hamaoka, M Kitagawa
PMCID: PMC1455793  PMID: 4994119

Abstract

To study the mechanism of antigenic stimulation in the secondary immune response, primed lymphoid cells were stimulated with antigen in various ways in vitro. The effect of antigenic stimulation was assessed by the antibody titres obtained after in vivo culture of primed cells in X-irradiated recipients.

Primed cells were much more effectively stimulated by antigen—antibody (Ag—Ab) complex than by free antigen. Primed cells could also be stimulated by spleen or lymph node cells from normal mice which had been exposed to free antigen or Ag—Ab complex in vitro or in vivo and thoroughly washed. Under these conditions, Ag—Ab complex was again much more effective than free antigen. When the cells were incubated with Ag—Ab complex, the dose of antigen bound to the cells was somewhat increased. But this increased binding of antigen could not solely account for the increase in immunogenicity.

It is suggested that the ingestion of antigen by macrophages is facilitated by the presence of antibody and that the macrophages mediate the effective immune stimulus to memory cells.

The effect of antibody in increasing the immunogenicity of antigen was lost completely when antibody was digested with pepsin. Thus, the Fc portion of antibody seemed to be important for this effect. However, it was demonstrated that antibody does not operate by becoming attached to macrophages as cytophilic antibody, and that complement is not involved in this process. The augmenting mechanism of antibody on the antigenic stimulation mediated by macrophages was discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed] [Google Scholar]
  2. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  3. Cohen M. W., Jacobson E. B., Thorbecke G. J. Gamma-globulin and antibody formation in vitro. V. The secondary response made by splenic white and red pulp with reference to the role of secondary nodules. J Immunol. 1966 Jun;96(6):944–952. [PubMed] [Google Scholar]
  4. FISHMAN M. Antibody formation in vitro. J Exp Med. 1961 Dec 1;114:837–856. doi: 10.1084/jem.114.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallily R., Feldman M. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology. 1967 Feb;12(2):197–206. [PMC free article] [PubMed] [Google Scholar]
  7. Gigli I., Nelson R. A., Jr Complement dependent immune phagocytosis. I. Requirements for C'1, C'4, C'2, C'3. Exp Cell Res. 1968 Jul;51(1):45–67. doi: 10.1016/0014-4827(68)90158-4. [DOI] [PubMed] [Google Scholar]
  8. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamaoka T., Kitagawa M., Matsuoka Y., Yamamura Y. Antibody production in mice. I. The analysis of immunological memory. Immunology. 1969 Jul;17(1):55–69. [PMC free article] [PubMed] [Google Scholar]
  10. Harris G. Studies of the mechanism of antigen stimulation of DNA synthesis in rabbit spleen cultures. Immunology. 1965 Dec;9(6):529–541. [PMC free article] [PubMed] [Google Scholar]
  11. ISHIZAKA K., CAMPBELL D. H. Biologic activity of soluble antigen-antibody complexes. V. Change of optical rotation by the formation of skin reactive complexes. J Immunol. 1959 Sep;83:318–326. [PubMed] [Google Scholar]
  12. Lang P. G., Ada G. L. Antigen in tissues. IV. The effect of antibody on the retention and localization of antigen in rat lymph nodes. Immunology. 1967 Nov;13(5):523–534. [PMC free article] [PubMed] [Google Scholar]
  13. Mitchison N. A. The immunogenic capacity of antigen taken up by peritoneal exudate cells. Immunology. 1969 Jan;16(1):1–14. [PMC free article] [PubMed] [Google Scholar]
  14. Nossal G. J., Ada G. L., Austin C. M., Pye J. Antigens in immunity. 8. Localization of 125-I-labelled antigens in the secondary response. Immunology. 1965 Oct;9(4):349–357. [PMC free article] [PubMed] [Google Scholar]
  15. PATTERSON R., SUSZKO I. M., PRUZANSKY J. J. In vitro uptake of antigen-antibody complexes by phagocytic cells. J Immunol. 1962 Oct;89:471–482. [PubMed] [Google Scholar]
  17. Pribnow J. F., Silverman M. S. Studies on the radiosensitive phase of the primary antibody response in rabbits. I. The role of the macrophage. J Immunol. 1967 Feb;98(2):225–229. [PubMed] [Google Scholar]
  18. RABINOWITZ Y. SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS. Blood. 1964 Jun;23:811–828. [PubMed] [Google Scholar]
  19. SORKIN E., BOYDEN S. V. Studies on the fate of antigens in vitro. I. The effect of specific antibody on the fate of I 131 trace labeled human serum albumin in vitro in the presence of guinea pig monocytes. J Immunol. 1959 Apr;82(4):332–339. [PubMed] [Google Scholar]
  20. Uhr J. W. Passive sensitization of lymphocytes and macrophages by antigen-antibody complexes. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1599–1606. doi: 10.1073/pnas.54.6.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WEIGLE W. O. Elimination of antigen-antibody complexes from sera of rabbits. J Immunol. 1958 Sep;81(3):204–213. [PubMed] [Google Scholar]
  22. Wakefield J. D., Thorbecke G. J. Relationship of germinal centers in lymphoid tissue to immunological memory. I. Evidence for the formation of small lymphocytes upon transfer of primed splenic white pulp to syngeneic mice. J Exp Med. 1968 Jul 1;128(1):153–169. doi: 10.1084/jem.128.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wakefield J. D., Thorbecke G. J. Relationship of germinal centers in lymphoid tissue to immunological memory. II. The detection of primed cells and their proliferation upon cell transfer to lethally irradiated syngeneic mice. J Exp Med. 1968 Jul 1;128(1):171–187. doi: 10.1084/jem.128.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]

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