Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 May;122(4):415–420. doi: 10.1042/bj1220415

The substrate specificity of purine phosphoribosyltransferases in Schizosaccharomyces pombe

A De Groodt 1, E P Whitehead 1, H Heslot 1, L Poirier 1
PMCID: PMC1176796  PMID: 5123876

Abstract

1. The activities of the purine phosphoribosyltransferases (EC 2.4.2.7 and 2.4.2.8) in purine-analogue-resistant mutants of Schizosaccharomyces pombe were checked. An 8-azathioxanthine-resistant mutant lacked hypoxanthine phosphoribosyltransferase, xanthine phosphoribosyltransferase and guanine phosphoribosyltransferase activities (EC 2.4.2.8) and appeared to carry a single mutation. Two 2,6-diaminopurine-resistant mutants retained these activities but lacked adenine phosphoribosyltransferase activity (EC 2.4.2.7). This evidence, together with data on purification and heat-inactivation patterns of phosphoribosyltransferase activities towards the various purines, strongly suggests that there are two phosphoribosyltransferase enzymes for purine bases in Schiz. pombe, one active with adenine, the other with hypoxanthine, xanthine and guanine. 2. Neither growth-medium supplements of purines nor mutations on genes involved in the pathway for new biosynthesis of purine have any influence on the amount of hypoxanthine–xanthine–guanine phosphoribosyltransferase produced by this organism.

Full text

PDF
415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATKINSON M. R., MURRAY A. W. INHIBITION OF PRUINE PHOSPHORIBOSYLTRANSFERASES OF EHRLICH ASCITES-TUMOUR CELLS BY 6-MERCAPTOPURINE. Biochem J. 1965 Jan;94:64–70. doi: 10.1042/bj0940064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adye J. C., Gots J. S. Further studies on genetically altered purine nucleotide pyrophosphorylases of Salmonella. Biochim Biophys Acta. 1966 May 5;118(2):344–350. doi: 10.1016/s0926-6593(66)80043-7. [DOI] [PubMed] [Google Scholar]
  3. Henderson J. F., Brox L. W., Kelley W. N., Rosenbloom F. M., Seegmiller J. E. Kinetic studies of hypoxanthine-guanine phosphoribosyltransferase. J Biol Chem. 1968 May 25;243(10):2514–2522. [PubMed] [Google Scholar]
  4. KALLE G. P., GOTS J. S. Alterations in purine nucleotide pyrophosphorylases and resistance to purine analogues. Biochim Biophys Acta. 1961 Oct 14;53:166–173. doi: 10.1016/0006-3002(61)90803-4. [DOI] [PubMed] [Google Scholar]
  5. KALLE G. P., GOTS J. S. GENETIC ALTERATION OF ADENYLIC PYROPHOSPHORYLASE IN SALMONELLA. Science. 1963 Nov 8;142(3593):680–681. doi: 10.1126/science.142.3593.680. [DOI] [PubMed] [Google Scholar]
  6. KORNBERG A., LIEBERMAN I., SIMMS E. S. Enzymatic synthesis of purine nucleotides. J Biol Chem. 1955 Jul;215(1):417–427. [PubMed] [Google Scholar]
  7. Kelley W. N., Rosenbloom F. M., Henderson J. F., Seegmiller J. E. Xanthine phosphoribosyltransferase in man: relationship to hypoxanthine-guanine phosphoribosyltransferase. Biochem Biophys Res Commun. 1967 Aug 7;28(3):340–345. doi: 10.1016/0006-291x(67)90315-4. [DOI] [PubMed] [Google Scholar]
  8. Krenitsky T. A., Papaioannou R., Elion G. B. Human hypoxanthine phosphoribosyltransferase. I. Purification, properties, and specificity. J Biol Chem. 1969 Mar 10;244(5):1263–1270. [PubMed] [Google Scholar]
  9. LEUPOLD U. Methodisches zur Genetik von Schizosaccharomyces pombe. Schweiz Z Pathol Bakteriol. 1955;18(5):1141–1146. [PubMed] [Google Scholar]
  10. LEUPOLD U. Physiologisch-genetische Studien an adenin-abhängigen Mutanten von Schizosaccharomyces pombe; ein Beitrag zum Problem der Pseudoallelie. Schweiz Z Pathol Bakteriol. 1957;20(5):535–544. [PubMed] [Google Scholar]
  11. LITTLEFIELD J. W. THE INOSINIC ACID PYROPHOSPHORYLASE ACTIVITY OF MOUSE FIBROBLASTS PARTIALLY RESISTANT TO 8-AZAGUANINE. Proc Natl Acad Sci U S A. 1963 Sep;50:568–573. doi: 10.1073/pnas.50.3.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Miller R. L., Bieber A. L. Purification and properties of inosine monophosphate: pyrophosphate phosphoribosyltransferase (EC 2.4.2.8) from brewers yeast. Biochemistry. 1968 Apr;7(4):1420–1426. doi: 10.1021/bi00844a026. [DOI] [PubMed] [Google Scholar]
  14. Rosenbloom F. M., Henderson J. F., Kelly W. N., Seegmiller J. E. Accelerated purine biosynthesis de novo in skin fibroblasts deficient in hypoxanthine--guanine phosphoribosyltransferase activity. Biochim Biophys Acta. 1968 Aug 23;166(1):258–260. doi: 10.1016/0005-2787(68)90512-1. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES