Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1970 Apr;5(4):446–450. doi: 10.1128/jvi.5.4.446-450.1970

Origin of Thymidine Kinase in Adenovirus-Infected Human Cell Lines

Saul Kit 1, K Nakajima 1, D R Dubbs 1
PMCID: PMC376025  PMID: 5452502

Abstract

Human adenovirus type 5 enhances the thymidine kinase activity of KB cells but does not induce the enzyme in kinase-deficient HeLa (BU25) cells. Vaccinia induces thymidine kinase activity in both KB and HeLa (BU25) cells. Human adenovirus types 2, 4, 7, and 12 also fail to induce the enzyme in HeLa (BU25) cells. Vaccinia replicates equally well in the presence or absence of HATG (hypoxanthine-aminopterin-thymidine-glycine) in KB and HeLa (BU25) cells. Adenovirus type 5 replicates in KB and in HeLa (BU25) cells in the absence of HATG, and adenovirus type 5 replicates in kinase-positive KB cells in the presence of HATG. However, replication of adenovirus type 5 is grossly inhibited in HeLa (BU25) cells in the presence of HATG. These results suggest that human adenoviruses do not code for a new virus-specific thymidine kinase.

Full text

PDF
446

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basilico C., Matsuya Y., Green H. Origin of the thymidine kinase induced by polyoma virus in productively infected cells. J Virol. 1969 Feb;3(2):140–145. doi: 10.1128/jvi.3.2.140-145.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bresnick E., Rapp F. Thymidine kinase activity in cells abortively and productively infected with human adenoviruses. Virology. 1968 Apr;34(4):799–802. doi: 10.1016/0042-6822(68)90102-5. [DOI] [PubMed] [Google Scholar]
  3. Buchan A., Watson D. H. The immunological specificity of thymidine kinases in cells infected by viruses of the herpes group. J Gen Virol. 1969 Apr;4(3):461–463. doi: 10.1099/0022-1317-4-3-461. [DOI] [PubMed] [Google Scholar]
  4. Carp R. I. Thymidine kinase from normal, simian virus 40-transformed and simian virus 40-lytically infected cells. J Virol. 1967 Oct;1(5):912–919. doi: 10.1128/jvi.1.5.912-919.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUBBS D. R., KIT S. ISOLATION AND PROPERTIES OF VACCINIA MUTANTS DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY. Virology. 1964 Feb;22:214–225. doi: 10.1016/0042-6822(64)90006-6. [DOI] [PubMed] [Google Scholar]
  6. DUBBS D. R., KIT S. MUTANT STRAINS OF HERPES SIMPLEX DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY. Virology. 1964 Apr;22:493–502. doi: 10.1016/0042-6822(64)90070-4. [DOI] [PubMed] [Google Scholar]
  7. DUBBS D. R., KIT S. THE EFFECT OF TEMPERATURE ON INDUCTION OF DEOXYTHYMIDINE KINASE ACTIVITY BY HERPERS SIMPLEX MUTANTS. Virology. 1965 Feb;25:256–270. doi: 10.1016/0042-6822(65)90204-7. [DOI] [PubMed] [Google Scholar]
  8. Dubbs D. R., Kit S., De Torres R. A., Anken M. Virogenic properties of bromodeoxyuridine-sensitive and bromodeoxyuridine-resistant simian virus 40-transformed mouse kidney cells. J Virol. 1967 Oct;1(5):968–979. doi: 10.1128/jvi.1.5.968-979.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  10. Hatanaka M., Dulbecco R. SV 40-specific thymidine kinase. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1888–1894. doi: 10.1073/pnas.58.5.1888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hatanaka M., Twiddy E., Gilden R. Electrophoresis of adenovirus-specified thymidine kinase. J Virol. 1969 Nov;4(5):801–803. doi: 10.1128/jvi.4.5.801-803.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KIT S., DUBBS D. R. PROPERTIES OF DEOXYTHYMIDINE KINASE PARTIALLY PURIFIED FROM NONINFECTED MOUSE FIBROBLAST CELLS. Virology. 1965 May;26:16–27. doi: 10.1016/0042-6822(65)90021-8. [DOI] [PubMed] [Google Scholar]
  13. Kit S., Dubbs D. R., Anken M. Altered properties of thymidine kinase after infection of mouse fibroblast cells with herpes simplex virus. J Virol. 1967 Feb;1(1):238–240. doi: 10.1128/jvi.1.1.238-240.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kit S., Dubbs D. R., DeTorres R. A., Melnick J. L. Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid". Virology. 1965 Nov;27(3):453–457. doi: 10.1016/0042-6822(65)90133-9. [DOI] [PubMed] [Google Scholar]
  15. Kit S., Dubbs D. R., Frearson P. M. HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity. Int J Cancer. 1966 Jan;1(1):19–30. doi: 10.1002/ijc.2910010105. [DOI] [PubMed] [Google Scholar]
  16. Kit S., Nakajima K., Kurimura T., Dubbs D. R., Cassingena R. Monkey-mouse hybrid cell lines containing the SV40 genome in a partially repressed state. Int J Cancer. 1970 Jan 15;5(1):1–14. doi: 10.1002/ijc.2910050102. [DOI] [PubMed] [Google Scholar]
  17. Kit S., Piekarski L. J., Dubbs D. R., De Torres R. A., Anken M. Enzyme induction in green monkey kidney cultures infected with simian adenovirus. J Virol. 1967 Feb;1(1):10–15. doi: 10.1128/jvi.1.1.10-15.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ledinko N. Enhanced deoxyribonucleic acid polymerase activity in human embryonic kidney cultures infected with adenovirus 2 or 12. J Virol. 1968 Feb;2(2):89–98. doi: 10.1128/jvi.2.2.89-98.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ledinko N., Fong C. K. Kinetics of nucleic acid synthesis in human embryonic kidney cultures infected with adenovirus 2 or 12: inhibition of cellular deoxyribonucleic acid synthesis. J Virol. 1969 Aug;4(2):123–132. doi: 10.1128/jvi.4.2.123-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ledinko N. Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by Adenovirus 2 or 12. Cancer Res. 1967 Aug;27(8):1459–1469. [PubMed] [Google Scholar]
  21. Littlefield J. W., Basilico C. Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature. 1966 Jul 16;211(5046):250–252. doi: 10.1038/211250a0. [DOI] [PubMed] [Google Scholar]
  22. Ogino T., Takahashi M. Altered properties of thymidine kinase induced in hamster kidney cells by adenovirus types 5 and 12. Biken J. 1969 Mar;12(1):17–23. [PubMed] [Google Scholar]
  23. Piña M., Green M. Biochemical studies on adenovirus multiplication. XIV. Macromolecule and enzyme synthesis in cells replicating oncogenic and nononcogenic human adenovirus. Virology. 1969 Aug;38(4):573–586. doi: 10.1016/0042-6822(69)90178-0. [DOI] [PubMed] [Google Scholar]
  24. Takahashi M., Ueda S., Ogino T. Enhancement of the thymidine kinase activity of human embryonic kidney cells and newborn hamster kidney cells by infection with human adenovirus types 5 and 12. Virology. 1966 Dec;30(4):742–743. doi: 10.1016/0042-6822(66)90181-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES