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. 1970 Nov;20(5):704–709. doi: 10.1128/am.20.5.704-709.1970

Comparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria

II. Skin Cross-Reactivity in Sensitized Guinea Pigs

S Landi 1, H R Held 1
PMCID: PMC377029  PMID: 5485083

Abstract

A study on skin cross-reactivity between stabilized 14C-labeled mycobacterial antigens, namely tuberculin purified protein derivative (PPD; from Mycobacterium tuberculosis), PPD-A (M. avium), PPD-Y (M. kansasii), PPD-G (M. scrofulaceum), PPD-B (M. intracellulare), and PPD-F (M. fortuitum), has been carried out in groups of guinea pigs sensitized with one of the following heat-killed mycobacteria: M. tuberculosis, M. avium, M. kansasii, M. scrofulaceum, M. intracellulare, or M. fortuitum. For each type of sensitization, the average response for the corresponding PPD antigen was higher than the average response for any of the other antigens. However, the responses to the heterologous PPD antigens were not necessarily significantly different among themselves, and the significant differences of the heterologous PPD antigens were distributed differently according to the type of sensitization. Therefore, 14C-PPD antigens skin cross-reacted in guinea pigs essentially in the same manner as reported by others for nonradioactive PPD antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EDWARDS L. B., PALMER C. E., AFFRONTI L. F., HOPWOOD L., EDWARDS P. Q. Epidemiologic studies of tuberculin sensitivity. II. Response to experimental infection with myobacteria isolated from human sources. Am J Hyg. 1960 Mar;71:218–241. [PubMed] [Google Scholar]
  2. Edwards L. B., Acquaviva F. A., Livesay V. T., Cross F. W., Palmer C. E. An atlas of sensitivity to tuberculin, PPD-B, and histoplasmin in the United States. Am Rev Respir Dis. 1969 Apr;99(4 Suppl):1–132. [PubMed] [Google Scholar]
  3. Landi S., Held H. R., Hauschild A. H., Hilsheimer R. Adsorption of tuberculin PPD to glass and plastic surfaces. Bull World Health Organ. 1966;35(4):593–602. [PMC free article] [PubMed] [Google Scholar]
  4. Landi S., Held H. R., Tseng M. C. Comparative study of 14C-labeled purified protein derivative from various mycobacteria. I. Preparation of 14C-labeled purified protein derivative antigens and their adsorption to glass. Appl Microbiol. 1970 Nov;20(5):696–703. doi: 10.1128/am.20.5.696-703.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MAGNUSSON M., ENGBAEK H. C., BENTZON M. W. Specificity of mycobacterial sensitins. II. Studies in guinea pigs with purified sensitin prepared from unclassified acid-fast bacilli. Am Rev Respir Dis. 1961 Jan;83:69–84. doi: 10.1164/arrd.1961.83.1.69. [DOI] [PubMed] [Google Scholar]
  6. MAGNUSSON M. Specificity of mycobacterial sensitins. I. Studies in guinea pigs with purified "tuberculin" prepared from mammalian and avian tubercle bacilli, Mycobacterium balnei, and other acid-fast bacilli. Am Rev Respir Dis. 1961 Jan;83:57–68. doi: 10.1164/arrd.1961.83.1.57. [DOI] [PubMed] [Google Scholar]
  7. PALMER C. E., EDWARDS L. B., HOPWOOD L., EDWARDS P. Q. Experimental and epidemiologic basis for the interpretation of tuberculin sensitivity. J Pediatr. 1959 Oct;55:413–429. doi: 10.1016/s0022-3476(59)80280-8. [DOI] [PubMed] [Google Scholar]
  8. RUNYON E. H. Anonymous mycobacteria in pulmonary disease. Med Clin North Am. 1959 Jan;43(1):273–290. doi: 10.1016/s0025-7125(16)34193-1. [DOI] [PubMed] [Google Scholar]
  9. Wayne L. G., Runyon E. H., Kubica G. P. Mycobacteria: a guide to nomenclatural usage. Am Rev Respir Dis. 1969 Nov;100(5):732–734. doi: 10.1164/arrd.1969.100.5.732. [DOI] [PubMed] [Google Scholar]

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