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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1982 Oct;63(5):472–478.

Polypeptide synthesis in columnar and squamous explants of human uterine cervix.

M E Cowan, K Ward, C B Woodman, G R Skinner
PMCID: PMC2040760  PMID: 6184064

Abstract

There were quantitative and qualitative differences in the in-vitro synthesis of 3 polypeptides between squamous and columnar epithelial explants of human cervix. One cross-linked keratin-like polypeptide of mol. wt 50,000 was synthesized and phosphorylated by squamous but not by columnar explants; a second cross-linked keratin-like polypeptide of mol. wt 52,000, which was present in larger amounts in squamous than columnar explants, was both glycosylated and phosphorylated during in-vitro explantation of squamous tissue; a third polypeptide of mol. wt 25,200 which was keratin-like but not cross-linked, was synthesized in squamous-tissue explants but in only 4% of columnar-tissue explants.

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Selected References

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  1. Birch J., Fink C. G., Skinner G. R., Thomas G. H., Jordan J. A. Replication of type 2 herpes simplex virus in human endocervical tissue in organ culture. Br J Exp Pathol. 1976 Aug;57(4):460–471. [PMC free article] [PubMed] [Google Scholar]
  2. FORAKER A. G. Histochemistry of the uterine cervix: normal exocevical, metaplastic, dysplastic, intraepithelial, and invasive squamous carcinomatous epithelium. Ann N Y Acad Sci. 1962 Sep 29;97:632–637. doi: 10.1111/j.1749-6632.1962.tb34674.x. [DOI] [PubMed] [Google Scholar]
  3. Fuchs E., Green H. The expression of keratin genes in epidermis and cultured epidermal cells. Cell. 1978 Nov;15(3):887–897. doi: 10.1016/0092-8674(78)90273-8. [DOI] [PubMed] [Google Scholar]
  4. Harper L., Bedson H. S., Buchan A. Identification of orthopoxviruses by polyacrylamide gel electrophoresis of intracellular polypeptides. I. Four major groupings. Virology. 1979 Mar;93(2):435–444. doi: 10.1016/0042-6822(79)90247-2. [DOI] [PubMed] [Google Scholar]
  5. Philipp E. Symposium on scanning electron microscopy of fertility and infertility, Eighth World Congress of Fertility and Sterility, Buenos Aires, Argentina, November 1974. Normal endocervical endothelium. J Reprod Med. 1975 May;14(5):188–191. [PubMed] [Google Scholar]
  6. Sun T. T., Green H. Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation. J Biol Chem. 1978 Mar 25;253(6):2053–2060. [PubMed] [Google Scholar]
  7. Sun T. T., Shih C., Green H. Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2813–2817. doi: 10.1073/pnas.76.6.2813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. VANTSIS J. T., WILDY P. Interaction of herpes virus and HeLa cells: comparison of cell killing and infective center formation. Virology. 1962 Jun;17:225–232. doi: 10.1016/0042-6822(62)90112-5. [DOI] [PubMed] [Google Scholar]
  9. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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