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. 1984 Jul;52(3):539–546.

An anti-mouse macrophage monoclonal antibody reacting with T-derived leukaemic cells.

A Martin, A M Chevrinais, D Bourel, R Fauchet, B Genetet, L Toujas
PMCID: PMC1454483  PMID: 6204931

Abstract

Hybridomas secreting anti-mouse macrophage antibodies were obtained by fusing a murine plasmacytoma with lymphocytes of a rat immunized against mouse macrophages. An IgM, monoclonal antibody (3 A 35) reacted with mouse monocytes, macrophages and polymorphonuclear leucocytes. It did not bind appreciably to erythrocytes, platelets and unstimulated T or B lymphocytes. However, 3 A 35 bound to various murine T-derived leukaemic cells and to a small proportion of Con A-stimulated thymocytes. Cross absorption experiments confirmed the existence of a common antigenic determinant on macrophages and leukaemic cells. The possibility that 3 A 35 identified a previously described antigen common to macrophages and normal or leukaemic T cells was investigated. The antibody was tested against thymocytes and macrophages of various mouse strains, some congenic for H-2 or T1a. The 3 A 35-detected antigen was found to be different from Ly5, Tla and Qa and did not represent a I-J encoded allotypic specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M., Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805–815. doi: 10.1002/eji.1830111013. [DOI] [PubMed] [Google Scholar]
  2. Ballieux R. E., Heijnen C. J. Immunoregulatory T cell subpopulations in man: dissection by monoclonal antibodies and Fc-receptors. Immunol Rev. 1983;74:5–28. doi: 10.1111/j.1600-065x.1983.tb01082.x. [DOI] [PubMed] [Google Scholar]
  3. Beller D. I., Springer T. A., Schreiber R. D. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982 Oct 1;156(4):1000–1009. doi: 10.1084/jem.156.4.1000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bourel D., Fauchet R., Genetet B., Merdrignac G., Hurez D. A monoclonal antibody to the HLA-A3 alloantigen. Immunol Lett. 1982 Nov;5(5):233–237. doi: 10.1016/0165-2478(82)90105-5. [DOI] [PubMed] [Google Scholar]
  5. Eisenbarth G. S., Haynes B. F., Schroer J. A., Fauci A. S. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J Immunol. 1980 Mar;124(3):1237–1244. [PubMed] [Google Scholar]
  6. Evan G. I., Lennox E. S., Alderson T., Croft L. A monoclonal anti-HLA antibody recognizes a mouse tumor-associated antigen. Eur J Immunol. 1983 Feb;13(2):160–166. doi: 10.1002/eji.1830130213. [DOI] [PubMed] [Google Scholar]
  7. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  8. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Habu S., Yamauchi K., Gershon R. K., Murphy D. B. A non-T:non-B cell bears I-A, I-E, I-J, and Tla (Qa-1?) determinants. Immunogenetics. 1981;13(3):215–225. doi: 10.1007/BF00350788. [DOI] [PubMed] [Google Scholar]
  10. Ho M. K., Springer T. A. Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J Immunol. 1982 Mar;128(3):1221–1228. [PubMed] [Google Scholar]
  11. LeBien T. W., Kersey J. H. A monoclonal antibody (TA-1) reactive with human T lymphocytes and monocytes. J Immunol. 1980 Nov;125(5):2208–2214. [PubMed] [Google Scholar]
  12. LeBlanc P. A., Russell S. W., Chang S. M. Mouse mononuclear phagocyte antigenic heterogeneity detected by monoclonal antibodies. J Reticuloendothel Soc. 1982 Sep;32(3):219–231. [PubMed] [Google Scholar]
  13. Mantovani A., Jerrells T. R., Dean J. H., Herberman R. B. Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer. 1979 Jan 15;23(1):18–27. doi: 10.1002/ijc.2910230105. [DOI] [PubMed] [Google Scholar]
  14. Martin A., Toujas L., Le Garrec Y., Dazord L., Amice J. Resistance to tumor graft in mice treated with inactivated Brucella abortus cultured in smooth or rough phase. J Natl Cancer Inst. 1979 Jan;62(1):123–127. [PubMed] [Google Scholar]
  15. McKenzie I. F., Potter T. Murine lymphocyte surface antigens. Adv Immunol. 1979;27:179–338. doi: 10.1016/s0065-2776(08)60263-1. [DOI] [PubMed] [Google Scholar]
  16. Metcalf D. Transformation of granulocytes to macrophages in bone marrow colonies in vitro. J Cell Physiol. 1971 Apr;77(2):277–280. doi: 10.1002/jcp.1040770216. [DOI] [PubMed] [Google Scholar]
  17. Murphy D. B., Herzenberg L. A., Okumura K., Herzenberg L. A., McDevitt H. O. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):699–712. doi: 10.1084/jem.144.3.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murphy G. F., Bhan A. K., Sato S., Mihm M. C., Jr, Harrist T. J. A new immunologic marker for human Langerhans cells. N Engl J Med. 1981 Mar 26;304(13):791–792. doi: 10.1056/NEJM198103263041320. [DOI] [PubMed] [Google Scholar]
  19. Niederhuber J. E., Allen P., Mayo L. The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response. J Immunol. 1979 Apr;122(4):1342–1349. [PubMed] [Google Scholar]
  20. OLD L. J., BOYSE E. A. ANTIGENIC PROPERTIES OF EXPERIMENTAL LEUKEMIAS. I. SEROLOGICAL STUDIES IN VITRO WITH SPONTANEOUS AND RADIATION-INDUCED LEUKEMIAS. J Natl Cancer Inst. 1963 Oct;31:977–995. [PubMed] [Google Scholar]
  21. Okumura K., Herzenberg L. A., Murphy D. B., McDevitt H. O., Herzenberg L. A. Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):685–698. doi: 10.1084/jem.144.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Old L. J., Boyse E. A., Stockert E. The G (Gross) leukemia antigen. Cancer Res. 1965 Jul;25(6):813–819. [PubMed] [Google Scholar]
  23. Rosenson R. S., Flaherty L., Reinisch C. L. Induction of surface Qa2 on lymphoid cells. J Immunol. 1981 Jun;126(6):2253–2257. [PubMed] [Google Scholar]
  24. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  25. Sun D., Lohmann-Matthes M. L. Functionally different subpopulations of mouse macrophages recognized by monoclonal antibodies. Eur J Immunol. 1982 Feb;12(2):134–140. doi: 10.1002/eji.1830120207. [DOI] [PubMed] [Google Scholar]
  26. Taniyama T., Watanabe T. Establishment of a hybridoma secreting a monoclonal antibody specific for activated tumoricidal macrophages. J Exp Med. 1982 Oct 1;156(4):1286–1291. doi: 10.1084/jem.156.4.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thivolet J., Schmitt D., Viac J., Brochier J. Les antigènes reconnus par les anticorps monoclonaux antilymphocytes peuvent avoir une localisation tissulaire inattendue. C R Seances Acad Sci III. 1983 Feb 7;296(5):203–206. [PubMed] [Google Scholar]
  28. Unkeless J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580–596. doi: 10.1084/jem.150.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]

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