Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Mar;153(3):1259–1265. doi: 10.1128/jb.153.3.1259-1265.1983

Oligopeptidase-deficient mutants of Salmonella typhimurium.

E R Vimr, L Green, C G Miller
PMCID: PMC221771  PMID: 6337992

Abstract

An oligopeptidase that hydrolyzes N-acetyl-L-alanyl-L-alanyl-L-alanyl-L-alanine (AcAla4) has been identified in extracts of Salmonella typhimurium. Mutants lacking this activity have been isolated in dcp mutant strains by screening extracts of mutagenized clones for failure to hydrolyze AcAla4 or by screening colonies for inability to use AcAla4 as a nitrogen source. Double mutants (dcp optA) lacking both oligopeptidase A and dipeptidyl carboxypeptidase cannot use AcAla4 as a nitrogen source, although dcp+ optA and dcp optA+ strains grow on this peptide. The mutations responsible for the loss of activity map at a locus (optA) between asd (75 map units) and xylA (78 map units). Oligopeptidase A hydrolyzes certain N-blocked tetrapeptides, unblocked pentapeptides, and unblocked hexapeptides, usually but not always liberating the C-terminal tripeptide. These two activities seem to be responsible for the production of a large fraction of the dipeptides that accumulate during protein breakdown in a pepN pepA pepB pepD strain.

Full text

PDF
1259

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheng Y. S., Zipser D., Cheng C. Y., Rolseth S. J. Isolation and characterization of mutations in the structural gene for protease III (ptr). J Bacteriol. 1979 Oct;140(1):125–130. doi: 10.1128/jb.140.1.125-130.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheng Y. S., Zipser D. Purification and characterization of protease III from Escherichia coli. J Biol Chem. 1979 Jun 10;254(11):4698–4706. [PubMed] [Google Scholar]
  3. Chumley F. G., Menzel R., Roth J. R. Hfr formation directed by tn10. Genetics. 1979 Apr;91(4):639–655. doi: 10.1093/genetics/91.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deutch C. E., Soffer R. L. Escherichia coli mutants defective in dipeptidyl carboxypeptidase. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5998–6001. doi: 10.1073/pnas.75.12.5998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. McHugh G. L., Miller C. G. Isolation and characterization of proline peptidase mutants of Salmonella typhimurium. J Bacteriol. 1974 Oct;120(1):364–371. doi: 10.1128/jb.120.1.364-371.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller C. G. Gentic mapping of Salmonella typhimurium peptidase mutations. J Bacteriol. 1975 Apr;122(1):171–176. doi: 10.1128/jb.122.1.171-176.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller C. G., Mackinnon K. Peptidase mutants of Salmonella typhimurium. J Bacteriol. 1974 Oct;120(1):355–363. doi: 10.1128/jb.120.1.355-363.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller C. G., Schwartz G. Peptidase-deficient mutants of Escherichia coli. J Bacteriol. 1978 Aug;135(2):603–611. doi: 10.1128/jb.135.2.603-611.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Payne J. W., Gilvarg C. Size restriction on peptide utilization in Escherichia coli. J Biol Chem. 1968 Dec 10;243(23):6291–6299. [PubMed] [Google Scholar]
  11. Simmonds S., Szeto K. S., Fletterick C. G. Soluble tri- and dipeptidases in Escherichia coli K-12+. Biochemistry. 1976 Jan 27;15(2):261–271. doi: 10.1021/bi00647a004. [DOI] [PubMed] [Google Scholar]
  12. Vimr E. R., Miller C. G. Dipeptidyl carboxypeptidase-deficient mutants of Salmonella typhimurium. J Bacteriol. 1983 Mar;153(3):1252–1258. doi: 10.1128/jb.153.3.1252-1258.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Walter R., Yoshimoto T. Postproline cleaving enzyme: kinetic studies of size and stereospecificity of its active site. Biochemistry. 1978 Oct 3;17(20):4139–4144. doi: 10.1021/bi00613a006. [DOI] [PubMed] [Google Scholar]
  14. Yen C., Green L., Miller C. G. Degradation of intracellular protein in Salmonella typhimurium peptidase mutants. J Mol Biol. 1980 Oct 15;143(1):21–33. doi: 10.1016/0022-2836(80)90122-9. [DOI] [PubMed] [Google Scholar]
  15. Yen C., Green L., Miller C. G. Peptide accumulation during growth of peptidase deficient mutants. J Mol Biol. 1980 Oct 15;143(1):35–48. doi: 10.1016/0022-2836(80)90123-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES