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. 1983 Sep;3(3):255–262. doi: 10.1007/BF00710951

Simultaneous determination of leu-enkephalin localization and [3H]γ-aminobutyric acid uptake in rat striatal cell cultures

Joseph E Mazurkiewicz 1,2, Anne Messer 1,2
PMCID: PMC11572923  PMID: 6367989

Abstract

  1. The purpose of this study was to investigate the coexistence ofγ-aminobutyric acid (GABA) and leu-enkephalin in single neurons from the corpus striatum.

  2. Monolayer cell cultures, started from newborn rat corpus striatum, were grown in serum-free medium and examined using GABA autoradiography and leu-enkephalin immunocytochemistry in a double-label protocol.

  3. Examples of cells were found which were positive for one or the other neurotransmitter or for neither transmitter, but not for both. Furthermore, cells which appear similar by morphological criteria alone differed in transmitter specificity.

  4. We conclude that the two transmitters tested are not localized within single cells and that morphology alone is inadequate to identify functional cell classes in this area.

Key words: cell culture, striatum, γ-aminobutyric acid, leu-enkephalin

References

  1. Bottenstein, J. E., and Sato, G. H. (1979). Growth of a rat neuroblastoma cell line in serum-free supplemented medium.Proc. Natl. Acad. Sci. USA76514–517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buck, S. H., and Yamamura, H. I. (1982). Neuropeptides in normal and pathological basal ganglia.Adv. Neurol.35121–132. [PubMed] [Google Scholar]
  3. Dahlstrom, A. (1968). Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat.Eur. J. Pharmacol.5111–113. [DOI] [PubMed] [Google Scholar]
  4. Elde, R., Hokfelt, T., Johanson, D., and Terenius, L. (1976). Immunohistochemical studies using antibodies to leucine enkephalin: Initial observations on the nervous system of the rat.Neuroscience5349–351. [DOI] [PubMed] [Google Scholar]
  5. Fahn, S. (1976). Biochemistry of the basal ganglia.Adv. Neurol.1459–88. [PubMed] [Google Scholar]
  6. Goldstein, A., Tachibama, S., Lowney, L. I., Hunkapiller, M., and Hood, L., (1979). Dynorphin-(1–13), an extraordinarily potent opioid peptide.Proc. Natl. Acad. Sci. USA766666–6670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldstein, A., Fischli, W., Lowney, L. I., Hunkapillar, M., and Hood, L. (1981). Porcine pituitary dynorphin: Complete amino acid sequence of the biologically active heptadecapeptide.Proc. Natl. Acad. Sci. USA787219–7223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hokfelt, T., Elde, R., Johansson, D., Terrenius, L., and Stein, L. (1977). The distribution of enkephalin immunoreactive cell bodies in rat central nervous system.Neurosci. Lett.525–31. [DOI] [PubMed] [Google Scholar]
  9. Hokfelt, T., Johansson, O., Ljungdahl, A., Lundberg, J. M., and Schultzbert, M. (1980). Peptidergic neurones.Nature284515–521. [DOI] [PubMed] [Google Scholar]
  10. Kakidani, H., Furutani, Y., Takahashi, H., Noda, M., Morimoto, Y., Hirose, T., Asai, M., Inayoma, S., Nakaniski, S., and Numa S. (1982). Cloning and sequence analysis of cDNA for porcine B-neo-endorphin/dynorphin precursor.Nature298245–249. [DOI] [PubMed] [Google Scholar]
  11. Kreutzbert, G. W. (1969). Neuronal dynamics and axonal flow, IV. Blockage of intra-neuronal enzyme transport by colchicine.Proc. Natl. Acad. Sci USA62722–728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Messer, A. (1981a). Primary monolayer cultures of the rat corpus striatum: Morphology and properties related to acetylcholine andγ-amino butyrate.Neuroscience62677–2687. [DOI] [PubMed] [Google Scholar]
  13. Messer, A. (1981b). Use of primary neuronal cultures to investigate neurological mutation at the cellular level. InGenetic Research Strategies for Psychobiology and Psychiatry (Gershon, E. S.,et al., Eds.), Boxwood Press, Pacific Grove, Calif., pp. 143–156. [Google Scholar]
  14. Messer, A., Mazurkiewicz, J., and Maskin, P. (1981). Growth of dissociated rat cerebellar cells using serum-free supplemented media and varied transferrin concentrations.Cell Mol. Neurobiol.199–114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller, R. J., and Pickel, V. M. (1980). The distribution and functions of the enkephalins.J. Histochem. Cytochem.28903–917. [DOI] [PubMed] [Google Scholar]
  16. Mori, S. (1966). Some observations on the fine structure of the corpus striatum of the rat brain.Z. Zeitschift Zellforschung70461–488. [DOI] [PubMed] [Google Scholar]
  17. Panula, P., Emson, P., and Wu, J.-Y. (1980). Demonstration of enkephalin-, substance P- and glutamate decarboxylase-like immunoreactivity in cultured cells derived from newborn rat neostriatum.Histochemistry69169–179. [DOI] [PubMed] [Google Scholar]
  18. Pickel, V. M., Sumai, K. K., Beady, S. C., Miller, R. J., and Reis, D. J. (1980). Immunocytochemical localization of enkephalin in the neostriatum of rat brain: A light and electron microscopic study.J. Comp. Neurol.189721–740. [DOI] [PubMed] [Google Scholar]
  19. Sar, M., Stumpf, W. A., Miller, R. J., Chang, K. J., and Cuatracasas P. (1978). Immunohistochemical localization of enkephalin in rat brain and spinal cord.J. Comp. Neurol.18217–38. [DOI] [PubMed] [Google Scholar]
  20. Schon, F., and Kelly, J. S. (1975). Selective uptake of3H-β-alanine by glia:Association with the glial uptake system for GABA.Brain Res.86243–257. [DOI] [PubMed] [Google Scholar]
  21. Somogyi, P., Priestley, J. V., Cuello, A. C., Smith, A. D., and Takagi, H. (1982). Synaptic connections of enkephalin-immunoreactive nerve terminals in the neostriatum: A correlated light and electron microscopic study.J. Neurocytol.11779–807. [DOI] [PubMed] [Google Scholar]
  22. Watson, S. J., Khachaturian, H., Akil, H., Coy, D. H., and Goldstein, A. (1982). Comparison of the distribution of dynorphin systems and enkephalin systems in the rat.Science2181134–1136. [DOI] [PubMed] [Google Scholar]
  23. Weber, E., and Barchas, J. D. (1983). Immunohistochemical distribution of dynorphin A andα-neo-endorphin systems.Proc. Natl. Acad. Sci. USA801125–1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weyhenmeyer, J. A., Raizida, M. K., Miller, R. J., and Fellows, R. F. (1980). Leucine-enkephalin immunoreactivity-localization in rat brain cells in culture.Brain Res.181465–469. [DOI] [PubMed] [Google Scholar]

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