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. 1983 Oct;18(4):834–836. doi: 10.1128/jcm.18.4.834-836.1983

Viability of Trichomonas vaginalis in vitro at four temperatures.

R F Smith
PMCID: PMC270915  PMID: 6605364

Abstract

The effect of temperature as a possible factor on the survival of Trichomonas vaginalis for shipment or routine laboratory maintenance was studied. Ten strains of T. vaginalis, ATCC 30001, ATCC 30238, and eight clinical isolates, were examined for viability when kept incubated at 37 degrees C or removed from this temperature and held at 42, 22, or 5 degrees C for increasing lengths of time without subculture or reincubation at 37 degrees C. The order in which the strains remained viable without subculture was: 5 degrees C, 8 to 10 days; 22 degrees C, 4 to 8 days; 37 degrees C, 4 to 6 days; 42 degrees C, less than 2 days. Vials of medium with cells were also held at 22 and 5 degrees C and then reincubated at 37 degrees C. Cultures held at 22 degrees C remained viable 6 to 8 days, whereas those stored at 5 degrees C remained viable 10 to 14 days. These data show that T. vaginalis withstands a wide range of temperatures, particularly below normal growth temperatures without subcultures, beyond what would be expected in mailing cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaulieu B. B., Jr, McLafferty M. A., Koch R. L., Goldman P. Metronidazole metabolism in cultures of Entamoeba histolytica and Trichomonas vaginalis. Antimicrob Agents Chemother. 1981 Sep;20(3):410–414. doi: 10.1128/aac.20.3.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DIAMOND L. S. The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol. 1957 Aug;43(4):488–490. [PubMed] [Google Scholar]
  3. Daly J. J., Baker M. L., Burton S. B. The sensitivity of Trichomonas vaginalis and Trichomonas gallinae to ultraviolet radiation. Photochem Photobiol. 1981 Feb;33(2):191–195. doi: 10.1111/j.1751-1097.1981.tb05323.x. [DOI] [PubMed] [Google Scholar]
  4. Fouts A. C., Kraus S. J. Trichomonas vaginalis: reevaluation of its clinical presentation and laboratory diagnosis. J Infect Dis. 1980 Feb;141(2):137–143. doi: 10.1093/infdis/141.2.137. [DOI] [PubMed] [Google Scholar]
  5. Hollander D. H. Colonial morphology of Trichomonas vaginalis in Agar. J Parasitol. 1976 Oct;62(5):826–828. [PubMed] [Google Scholar]
  6. Ivey M. H. Use of solid medium techniques to evaluate factors affecting the ability of Trichomonas vaginalis to survive freezing. J Parasitol. 1975 Dec;61(6):1101–1103. [PubMed] [Google Scholar]
  7. Krieger J. N., Rein M. F. Zinc sensitivity of Trichomonas vaginalis: in vitro studies and clinical implications. J Infect Dis. 1982 Sep;146(3):341–345. doi: 10.1093/infdis/146.3.341. [DOI] [PubMed] [Google Scholar]
  8. Kulda J., Vojtechovská M., Tachezy J., Demes P., Kunzová E. Metronidazole resistance of Trichomonas vaginalis as a cause of treatment failure in trichomoniasis--A case report. Br J Vener Dis. 1982 Dec;58(6):394–399. doi: 10.1136/sti.58.6.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lumsden W. H., Robertson D. H., McNeillage G. J. Isolation, cultivation, low temperature preservation, and infectivity titration of Trichomonas vaginalis. Br J Vener Dis. 1966 Sep;42(3):145–154. doi: 10.1136/sti.42.3.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mason P. R. Trichomoniasis. New ideas on an old disease. S Afr Med J. 1980 Nov 22;58(21):857–859. [PubMed] [Google Scholar]
  11. Matthews R. S., Daly J. J. Trichomonas gallinae: use of solid medium to test survival under various environmental conditions. Exp Parasitol. 1974 Oct;36(2):288–298. doi: 10.1016/0014-4894(74)90068-x. [DOI] [PubMed] [Google Scholar]
  12. Meingassner J. G., Nesvadba H., Mieth H. New chemotherapeutic nitroheterocycles active against 5-nitroimidazole-resistant strains of trichomonads. Arzneimittelforschung. 1981;31(1):6–8. [PubMed] [Google Scholar]
  13. Müller M., Meingassner J. G., Miller W. A., Ledger W. J. Three metronidazole-resistant strains of Trichomonas vaginalis from the United States. Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 1):808–812. doi: 10.1016/s0002-9378(16)32741-7. [DOI] [PubMed] [Google Scholar]
  14. Smith R. F., Di Domenico A. Measuring the in vitro susceptibility of Trichomonas vaginalis to metronidazole: a disk broth method. Sex Transm Dis. 1980 Jul-Sep;7(3):120–124. doi: 10.1097/00007435-198007000-00005. [DOI] [PubMed] [Google Scholar]
  15. WHITTINGTON M. J. The survival of Trichomonas vaginalis at temperatures below +37 degrees C. J Hyg (Lond) 1951 Dec;49(4):400–409. doi: 10.1017/s0022172400066717. [DOI] [PMC free article] [PubMed] [Google Scholar]

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