Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jun;40(3):1075–1082. doi: 10.1128/iai.40.3.1075-1082.1983

Lack of antibodies to human heart tissue in sera of rhesus monkeys immunized with Streptococcus mutans antigens and comparative study with rabbit antisera.

L A Bergmeier, T Lehner
PMCID: PMC348160  PMID: 6852912

Abstract

Immunization of rabbits and rhesus monkeys with Streptococcus mutans whole cells, cell walls, and defined streptococcal antigens (SAs) SA I/II, SA I, and SA II resulted in high antibody titers to SA I/II (10(-4) to 10(-6)) when tested by the solid-phase radioimmunoassay. Cross-reactive antibodies to heart homogenates (HH) were not elicited in rhesus monkeys. A few rabbits immunized with cell wall or SA II preparation in Freund complete adjuvant followed by the incomplete adjuvant yielded low antibody titers (up to 10(-2)) to the HH. The specificity of the putative heart cross-reactive antibodies was tested by immunoadsorption with related and unrelated antigens. Whereas antibody to SA I/II showed specific adsorption with SA I/II but not with HH, immunoglobulin G, or the unrelated antigens, antibody to HH seemed to have been adsorbed with all of the related and unrelated antigens. There was no evidence for the development of heart cross-reactive antibodies on immunization of rhesus monkeys or rabbits with SA I/II and aluminium hydroxide or Freund incomplete adjuvant, administered by the subcutaneous or intramuscular route in doses of up to 13 mg of the immunogen.

Full text

PDF
1075

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowden G. H., Hardie J. M., Fillery E. D. Antigens from Actinomyces species and their value in identification. J Dent Res. 1976 Jan;55:A192–A204. doi: 10.1177/002203457605500112011. [DOI] [PubMed] [Google Scholar]
  2. Bowen W. H., Cohen B., Cole M. F., Colman G. Immunization against dental caries. Br Dent J. 1975 Jul 15;139(2):45–58. [PubMed] [Google Scholar]
  3. Christensen P., Schalén C., Holm S. E. Reevaluation of experiments intended to demonstrate immunological cross-reactions between mammalian tissues and streptococci. Prog Allergy. 1979;26:1–41. doi: 10.1159/000314455. [DOI] [PubMed] [Google Scholar]
  4. FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferretti J. J., Shea C., Humphrey M. W. Cross-reactivity of Streptococcus mutans antigens and human heart tissue. Infect Immun. 1980 Oct;30(1):69–73. doi: 10.1128/iai.30.1.69-73.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Hughes M., Machardy S. M., Sheppard A. J., Woods N. C. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infect Immun. 1980 Feb;27(2):576–588. doi: 10.1128/iai.27.2.576-588.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lamb J. R., Kontiainen S., Lehner T. A comparative investigation of the generation of specific T cell-helper function induced by Streptococcus mutans in monkeys and mice. J Immunol. 1980 May;124(5):2384–2389. [PubMed] [Google Scholar]
  9. Lamb J. R., Kontiainen S., Lehner T. Generation of specfic T-cell suppressor function induced by Streptococcus mutans in monkeys and mice. Infect Immun. 1979 Dec;26(3):903–909. doi: 10.1128/iai.26.3.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lehner T., Challacombe S. J., Caldwell J. An experimental model for immunological studies of dental caries in the rhesus monkey. Arch Oral Biol. 1975 May-Jun;20(5-6):299–304. doi: 10.1016/0003-9969(75)90018-7. [DOI] [PubMed] [Google Scholar]
  11. Lehner T., Challacombe S. J., Caldwell J. Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys. Nature. 1975 Apr 10;254(5500):517–520. doi: 10.1038/254517a0. [DOI] [PubMed] [Google Scholar]
  12. Lehner T., Challacombe S. J., Wilton J. M., Caldwell J. Cellular and humoral immune responses in vaccination against dental caries in monkeys. Nature. 1976 Nov 4;264(5581):69–72. doi: 10.1038/264069a0. [DOI] [PubMed] [Google Scholar]
  13. Lehner T., Lamb J. R., Welsh K. L., Batchelor R. J. Association between HLA-DR antigens and helper cell activity in the control of dental caries. Nature. 1981 Aug 20;292(5825):770–772. doi: 10.1038/292770a0. [DOI] [PubMed] [Google Scholar]
  14. Lehner T., Russell M. W., Caldwell J. Immunisation with a purified protein from Streptococcus mutans against dental caries in rhesus monkeys. Lancet. 1980 May 10;1(8176):995–996. doi: 10.1016/s0140-6736(80)91437-3. [DOI] [PubMed] [Google Scholar]
  15. Lehner T., Russell M. W., Caldwell J., Smith R. Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect Immun. 1981 Nov;34(2):407–415. doi: 10.1128/iai.34.2.407-415.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lehner T. The relationship between human helper and suppressor factors to a streptococcal protein antigen. J Immunol. 1982 Nov;129(5):1936–1940. [PubMed] [Google Scholar]
  17. Michalek S. M., McGhee J. R., Mestecky J., Arnold R. R., Bozzo L. Ingestion of Streptococcus mutans induces secretory immunoglobulin A and caries immunity. Science. 1976 Jun 18;192(4245):1238–1240. doi: 10.1126/science.1273589. [DOI] [PubMed] [Google Scholar]
  18. Michalek S. M., McGhee J. R., Shiota T., Devenyns D. Virulence of Streptococcus mutans: cariogenicity of S. mutans in adult gnotobiotic rats. Infect Immun. 1977 Feb;15(2):466–471. doi: 10.1128/iai.15.2.466-471.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Russell M. W., Lehner T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol. 1978;23(1):7–15. doi: 10.1016/0003-9969(78)90047-x. [DOI] [PubMed] [Google Scholar]
  21. Russell M. W., Zanders E. D., Bergmeier L. A., Lehner T. Affinity purification and characterization of protease-susceptible antigen I of Streptococcus mutans. Infect Immun. 1980 Sep;29(3):999–1006. doi: 10.1128/iai.29.3.999-1006.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Russell R. R., Beighton D., Cohen B. Immunisation of monkeys (Macaca fascicularis) with antigens purified from Streptococcus mutans. Br Dent J. 1982 Feb 2;152(3):81–84. doi: 10.1038/sj.bdj.4804751. [DOI] [PubMed] [Google Scholar]
  23. Smith D. J., Taubman M. A., Ebersole J. L. Effects of local immunization with glucosyltransferase on colonization of hamsters by Streptococcus mutans. Infect Immun. 1982 Aug;37(2):656–661. doi: 10.1128/iai.37.2.656-661.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Smith R., Lehner T. A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans. Clin Exp Immunol. 1981 Feb;43(2):417–424. [PMC free article] [PubMed] [Google Scholar]
  25. Talbman M. A., Smith D. J. Effects of local immunization with Streptococcus mutans on induction of salivary immunoglobulin A antibody and experimental dental caries in rats. Infect Immun. 1974 Jun;9(6):1079–1091. doi: 10.1128/iai.9.6.1079-1091.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Van de Rijn I., Bleiweis A. S., Zabriskie J. B. Antigens in Streptococcus mutans cross reactive with human heart muscle. J Dent Res. 1976 Apr;55(Spec No):C59–C64. doi: 10.1177/002203457605500326011. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES