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. 1982 Sep;79(18):5460–5464. doi: 10.1073/pnas.79.18.5460

Gamma-irradiation activates biochemical systems: induction of nitrate reductase activity in plant callus.

K N Pandey, P S Sabharwal
PMCID: PMC346923  PMID: 6957874

Abstract

Gamma-irradiation induced high levels of nitrate reductase activity (NADH:nitrate oxidoreductase, EC 1.6.6.1) in callus of Haworthia mirabilis Haworth. Subcultures of gamma-irradiated tissues showed autonomous growth on minimal medium. We were able to mimic the effects of gamma-irradiation by inducing nitrate reductase activity in unirradiated callus with exogenous auxin and kinetin. These results revealed that induction of nitrate reductase activity by gamma-irradiation is mediated through in vitro activation of hormone synthesis in callus cells.

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Selected References

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