Abstract
Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30 cell separator and an Aminco Celltrifuge. The best results were achieved using the paediatric pheresis set on the Hemonetics model 30. Marrow was frozen in 120 ml aliquots in a programmed freezer with rapid cooling of the freezing chamber during the phase change from the liquid to the solid state. After freeze-thawing the average nucleated cell recovery was approximately 50% and the progenitor cell recovery 80%.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Appelbaum F. R., Herzig G., Graw R. G., Zeigler J. L. Accelerated hemopoietic recovery following the infusion of cryopreserved autologous bone marrow in humans. Exp Hematol. 1979;7 (Suppl 5):297–301. [PubMed] [Google Scholar]
- Burgess A. W., Wilson E. M., Metcalf D. Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells. Blood. 1977 Apr;49(4):573–583. [PubMed] [Google Scholar]
- Dicke K. A., Zander A., Spitzer G., Verma D. S., Peters L., Vellekoop L., McCredie K. B., Hester J. Autologous bone-marrow transplantation in relapsed adult acute leukaemia. Lancet. 1979 Mar 10;1(8115):514–517. doi: 10.1016/s0140-6736(79)90943-7. [DOI] [PubMed] [Google Scholar]
- Lewis J. P., Passovoy M., Conti S. A., McFate P. A., Trobaugh F. E., Jr The effect of cooling regimens on the transplantation potential of marrow. Transfusion. 1967 Jan-Feb;7(1):17–32. doi: 10.1111/j.1537-2995.1967.tb04826.x. [DOI] [PubMed] [Google Scholar]
- PEGG D. E., KEMP N. H. Collection, storage, and administration of autologous human bone-marrow. Lancet. 1960 Dec 31;2(7166):1426–1429. doi: 10.1016/s0140-6736(60)92571-x. [DOI] [PubMed] [Google Scholar]
- Pike B. L., Robinson W. A. Human bone marrow colony growth in agar-gel. J Cell Physiol. 1970 Aug;76(1):77–84. doi: 10.1002/jcp.1040760111. [DOI] [PubMed] [Google Scholar]
- WILSON R. E. Technics of human-bone-marrow procurement by aspiration from living donors. N Engl J Med. 1959 Oct 15;261:781–785. doi: 10.1056/NEJM195910152611602. [DOI] [PubMed] [Google Scholar]
- Weiner R. S., Tobias J. S., Yankee R. A. The processing of human bone marrow for cryopreservation and reinfusion. Biomedicine. 1976;24(4):226–231. [PubMed] [Google Scholar]
- van Bekkum D. W. The double barrier in bone marrow transplantation. Semin Hematol. 1974 Jul;11(3):325–340. [PubMed] [Google Scholar]