Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Jul;37(1):160–165. doi: 10.1128/iai.37.1.160-165.1982

Comparison of enzyme-linked immunosorbent assay and neutralization techniques for measurement of antibody to respiratory syncytial virus: implications for parenteral immunization with live virus vaccine.

R B Belshe, L P Van Voris, M A Mufson, E B Buynak, A A McLean, M A Hilleman
PMCID: PMC347505  PMID: 7107002

Abstract

The sensitivity of an enzyme-linked immunosorbent assay (ELISA) to detect low levels of antibody to respiratory syncytial (RS) virus was compared with a tube dilution neutralization test (NEUT) on sera obtained from children who received a parenteral live RS virus vaccine. Among the children who developed antibody in response to live RS virus vaccine. ELISA was as sensitive as NEUT at detecting antibody increases. Some children who did not have detectable prevaccine ELISA antibody possessed NEUT antibody; these children were generally less than 12 months old, suggesting that they had low levels of maternal antibody. Low levels of NEUT or ELISA antibody were associated with the absence of antibody increases after injection of live RS virus vaccine. The quantity of antibody stimulated by this live RS virus vaccine was small compared with that which was stimulated by naturally acquired RS virus infection. We concluded that ELISA is a satisfactory test for determining antibody to RS virus in vaccine field trials, given the understanding that low levels of preexisting antibody are not detected in some instances.

Full text

PDF
160

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrecht P., Ennis F. A., Saltzman E. J., Krugman S. Persistence of maternal antibody in infants beyond 12 months: mechanism of measles vaccine failure. J Pediatr. 1977 Nov;91(5):715–718. doi: 10.1016/s0022-3476(77)81021-4. [DOI] [PubMed] [Google Scholar]
  2. Belshe R. B., Van Voris L. P., Mufson M. A., Hyler L. Epidemiology of severe respiratory syncytial virus infections in Huntington, West Virginia. W V Med J. 1981 Mar;77(3):49–52. [PubMed] [Google Scholar]
  3. Belshe R. B., Van Voris L. P., Mufson M. A. Parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. J Infect Dis. 1982 Mar;145(3):311–319. doi: 10.1093/infdis/145.3.311. [DOI] [PubMed] [Google Scholar]
  4. Black F. L., Lamm S. H., Emmons J. E., Pinheiro F. P. Durability of antibody titers induced by RA27/3 rubella virus vaccine. J Infect Dis. 1978 Mar;137(3):322–323. doi: 10.1093/infdis/137.3.322. [DOI] [PubMed] [Google Scholar]
  5. Buynak E. B., Weibel R. E., Carlson A. J., McLean A. A., Hilleman M. R. Further investigations of live respiratory syncytial virus vaccine administered parenterally. Proc Soc Exp Biol Med. 1979 Feb;160(2):272–277. doi: 10.3181/00379727-160-40433. [DOI] [PubMed] [Google Scholar]
  6. Buynak E. B., Weibel R. E., McLean A. A., Hilleman M. R. Live respiratory syncytial virus vaccine administered parenterally. Proc Soc Exp Biol Med. 1978 Apr;157(4):636–642. doi: 10.3181/00379727-157-40112. [DOI] [PubMed] [Google Scholar]
  7. Buynak E. B., Weibel R. E., Mclean A. A., Hilleman M. K. Long-term persistence of antibody following Enders' original and More attenuated live measles virus vaccine. Proc Soc Exp Biol Med. 1976 Dec;153(3):441–443. doi: 10.3181/00379727-153-39564. [DOI] [PubMed] [Google Scholar]
  8. Gail M. H., Green S. B. A generalization of the one-sided two-sample Kolmogorov-Smirnov statistic for evaluating diagnostic tests. Biometrics. 1976 Sep;32(3):561–570. [PubMed] [Google Scholar]
  9. Kim H. W., Arrobio J. O., Brandt C. D., Wright P., Hodes D., Chanock R. M., Parrott R. H. Safety and antigenicity of temperature sensitive (TS) mutant respiratory syncytial virus (RSV) in infants and children. Pediatrics. 1973 Jul;52(1):56–63. [PubMed] [Google Scholar]
  10. Kim H. W., Arrobio J. O., Pyles G., Brandt C. D., Camargo E., Chanock R. M., Parrott R. H. Clinical and immunological response of infants and children to administration of low-temperature adapted respiratory syncytial virus. Pediatrics. 1971 Nov;48(5):745–755. [PubMed] [Google Scholar]
  11. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. Am J Epidemiol. 1969 Apr;89(4):422–434. doi: 10.1093/oxfordjournals.aje.a120955. [DOI] [PubMed] [Google Scholar]
  12. Leinikki P. O., Passila S. Quantitative, semiautomated, enzyme-linked immunosorbent assay for viral antibodies. J Infect Dis. 1977 Oct;136 (Suppl):S294–S299. doi: 10.1093/infdis/136.supplement_2.s294. [DOI] [PubMed] [Google Scholar]
  13. Mills J., 5th, Van Kirk J. E., Wright P. F., Chanock R. M. Experimental respiratory syncytial virus infection of adults. Possible mechanisms of resistance to infection and illness. J Immunol. 1971 Jul;107(1):123–130. [PubMed] [Google Scholar]
  14. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. Epidemiology of respiratory syncytial virus infection in Washington, D.C. II. Infection and disease with respect to age, immunologic status, race and sex. Am J Epidemiol. 1973 Oct;98(4):289–300. doi: 10.1093/oxfordjournals.aje.a121558. [DOI] [PubMed] [Google Scholar]
  15. Richardson L. S., Yolken R. H., Belshe R. B., Camargo E., Kim H. W., Chanock R. M. Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection. Infect Immun. 1978 Jun;20(3):660–664. doi: 10.1128/iai.20.3.660-664.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weibel R. E., Buynak E. B., McLean A. A., Hilleman M. R. Long-term follow-up for immunity after monovalent or combined live measles, mumps, and rubella virus vaccines. Pediatrics. 1975 Sep;56(3):380–387. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES