Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1976 May;83(2):255–268.

Mononuclear cell turnover in chronic inflammation: studies on tritiated thymidine-labeled cells in blood, tuberculin traps, and dermal BCG lesions of rabbits.

T Tsuda, A M Dannenberg, M Ando, H Abbey, A R Corrin
PMCID: PMC2032319  PMID: 773192

Abstract

Rabbits were injected intradermally in multiple sites with BCG. Four days after the BCG injection, they were injected intravenously with a single pulse of tritiated thymidine (3H-TdR) at various times thereafter, the BCG lesions were biopsied and evaluated for 3H-TdR-labeled mononuclear cells (MN). Periodically, Old Tuberculin (OT) was injected intradermally, creating MN traps which were biopsied and evaluated 1 day after their onset. 3H-TdR-labeled cells were also evaluated in samples of blood. During the first 8 days after the 3H-TdR pulse, the labeled MNs represent short-lived cells, i.e., recently dividing monocytes and lymphocytes. During this time, the percentage of labeled MNs in the blood, in the traps, and in the BCG lesions rose and fell together. This result suggests that the majority of the MNs in the BCG lesions had a turnover rate of about a week. By 12 days and afterward, the percentage of MNs labeled by 3H-TdR in the blood was higher than that in the BCG lesions which was in turn higher than that in the traps. At this time the circulating MN population probably contained labeled long-lived lymphocytes that did not enter inflammatory sites (the traps) as readily as the short-lived lymphocytes. The labeled MNs remaining in the BCG lesions probably did not divide and dilute out their 3H-TdR label as readily as those that were trapped from the bone marrow via the blood. The percentage of MNs labeled with 3H-TdR in the traps had decreased to about one-fifteenth of its peak value by 12 days, suggesting that the bone marrow's supply of labeled MNs was depleted at this time, except for the few cells labeled as a result of 3H-TdR reutilization.

Full text

PDF
255

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ando M., Dannenberg A. M., Jr, Courtade E., Shima K. Turnover of tritiated-thymidine-labeled mononuclear cells in tuberculous lesions of rabbits: a comparison of primary dermal BCG lesions and those of reinfection. Proc Soc Exp Biol Med. 1976 Mar;151(3):491–494. doi: 10.3181/00379727-151-39242. [DOI] [PubMed] [Google Scholar]
  2. Ando M., Dannenberg A. M., Jr Macrophage accumulation, division, maturation, and digestive and microbicidal capacities in tuberculous lesions. IV. Macrophage turnover, lysosomal enzymes, and division in healing lesions. Lab Invest. 1972 Nov;27(5):466–472. [PubMed] [Google Scholar]
  3. Ando M., Dannenberg A. M., Jr, Shima K. Macrophage accumulation, division, maturation and digestive and microbicidal capacities in tuberculous lesions. II. Rate at which mononuclear cells enter and divide in primary BCG lesions and those of reinfection. J Immunol. 1972 Jul;109(1):8–19. [PubMed] [Google Scholar]
  4. Ando M. Macrophage activation in tuberculin reactions of rabbits with primary BCG infection and reinfection. J Reticuloendothel Soc. 1973 Aug;14(2):132–145. [PubMed] [Google Scholar]
  5. Boggs D. R. The kinetics of neutrophilic leukocytes in health and in disease. Semin Hematol. 1967 Oct;4(4):359–386. [PubMed] [Google Scholar]
  6. Borges J. S., Johnson W. D., Jr Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products. J Exp Med. 1975 Feb 1;141(2):483–496. doi: 10.1084/jem.141.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CRONKITE E. P., FLIEDNER T. M. GRANULOCYTOPOIESIS. N Engl J Med. 1964 Jun 18;270:1347–CONTD. doi: 10.1056/NEJM196406182702506. [DOI] [PubMed] [Google Scholar]
  8. Chandrasekhar S., Shima K., Dannenberg A. M., Kambara T., Fabrikant J. I., Roessler W. G. Radiation, Infection, and Macrophage Function IV. Effect of Radiation on the Proliferative Abilities of Mononuclear Phagocytes in Tuberculous Lesions of Rabbits. Infect Immun. 1971 Feb;3(2):254–259. doi: 10.1128/iai.3.2.254-259.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dannenberg A. M., Jr Cellular hypersensitivity and cellular immunity in the pathogensis of tuberculosis: specificity, systemic and local nature, and associated macrophage enzymes. Bacteriol Rev. 1968 Jun;32(2):85–102. doi: 10.1128/br.32.2.85-102.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dannenberg A. M., Jr, Meyer O. T., Esterly J. R., Kambara T. The local nature of immunity in tuberculosis, illustrated histochemically in dermal BCG lesions. J Immunol. 1968 May;100(5):931–941. [PubMed] [Google Scholar]
  12. Feinendegen L. E., Heiniger H. J., Friedrich G., Cronkite E. P. Differences in reutilization of thymidine in hemopoietic and lymphopoietic tissues of the normal mouse. Cell Tissue Kinet. 1973 Nov;6(6):573–585. doi: 10.1111/j.1365-2184.1973.tb01648.x. [DOI] [PubMed] [Google Scholar]
  13. Jacoby F., Lee C. G. Long-term culture of mouse peritoneal macrophages with special reference to proliferation. J Physiol. 1969 Jul;203(1):20P–22P. [PubMed] [Google Scholar]
  14. KOSUNEN T. U., WAKSMAN B. H., FLAX M. H., TIHEN W. S. Radioautographic study of cellular mechanisms in delayed hypersensitivity. I. Delayed reactions to tuberculin and purified proteins in the rat and guinea-pig. Immunology. 1963 May;6:276–290. [PMC free article] [PubMed] [Google Scholar]
  15. Kambara T., Chandrasekhar S., Dannenberg A. M., Jr, Meyer O. T. Radiation, infection, and macrophage function. I. Effects of whole body radiation on dermal tuberculous lesions in rabbits: development, histology, and histochemistry. J Reticuloendothel Soc. 1970 Jan;7(1):53–78. [PubMed] [Google Scholar]
  16. Koster F. T., McGregor D. D., Mackaness G. B. The mediator of cellular immunity. II. Migration of immunologically committed lymphocytes into inflammatory exudates. J Exp Med. 1971 Feb 1;133(2):400–409. doi: 10.1084/jem.133.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Koster F. T., McGregor D. D. The mediator of cellular immunity. 3. Lymphocyte traffic from the blood into the inflamed peritoneal cavity. J Exp Med. 1971 Apr 1;133(4):864–876. doi: 10.1084/jem.133.4.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MITCHELL J., MCDONALD W., NOSSAL G. J. AUTORADIOGRAPHIC STUDIES OF THE IMMUNE RESPONSE. 3. DIFFERENTIAL LYMPHOPOIESIS IN VARIOUS ORGANS. Aust J Exp Biol Med Sci. 1963 Aug;41:SUPPL411–SUPPL421. doi: 10.1038/icb.1963.62. [DOI] [PubMed] [Google Scholar]
  19. Mackaness G. B. The immunology of antituberculous immunity. Am Rev Respir Dis. 1968 Mar;97(3):337–344. doi: 10.1164/arrd.1968.97.3.337. [DOI] [PubMed] [Google Scholar]
  20. Mauel J., Defendi V. Infection and transformation of mouse peritoneal macrophages by simian virus 40. J Exp Med. 1971 Aug 1;134(2):335–350. doi: 10.1084/jem.134.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McGregor D. D., Logie P. S. The mediator of cellular immunity. VII. Localization of sensitized lymphocytes in inflammatory exudates. J Exp Med. 1974 Jun 1;139(6):1415–1430. doi: 10.1084/jem.139.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. North R. J. The mitotic potential of fixed phagocytes in the liver as revealed during the development of cellular immunity. J Exp Med. 1969 Aug 1;130(2):315–326. doi: 10.1084/jem.130.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. North R. J. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. doi: 10.1084/jem.132.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Papadimitriou J. M., Spector W. G. The ultrastructure of high- and low-turnover inflammatory granulomata. J Pathol. 1972 Jan;106(1):37–43. doi: 10.1002/path.1711060104. [DOI] [PubMed] [Google Scholar]
  25. Shima K., Dannenberg A. M., Jr, Ando M., Chandrasekhar S., Seluzicki J. A., Fabrikant J. I. Macrophage accumulation, division, maturation, and digestive and microbicidal capacities in tuberculous lesions. I. Studies involving their incorporation of tritiated thymidine and their content of lysosomal enzymes and bacilli. Am J Pathol. 1972 Apr;67(1):159–180. [PMC free article] [PubMed] [Google Scholar]
  26. Smith J. B., Pedersen N. C., Morris B. The role of the lymphatic system in inflammatory responses. Ser Haematol. 1970;3(2):17–61. [PubMed] [Google Scholar]
  27. Spector W. G., Lykke A. W. The cellular evolution of inflammatory granulomata. J Pathol Bacteriol. 1966 Jul;92(1):163–167. doi: 10.1002/path.1700920117. [DOI] [PubMed] [Google Scholar]
  28. Spector W. G. The granulomatous inflammatory exudate. Int Rev Exp Pathol. 1969;8:1–55. [PubMed] [Google Scholar]
  29. VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
  30. Van Furth R., Diesselhoff-den Dulk M. C., Mattie H. Quantitative study on the production and kinetics of mononuclear phagocytes during an acute inflammatory reaction. J Exp Med. 1973 Dec 1;138(6):1314–1330. doi: 10.1084/jem.138.6.1314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Virolainen M., Defendi V. Dependence of macrophage growth in vitro upon interaction with other cell types. Wistar Inst Symp Monogr. 1967;7:67–85. [PubMed] [Google Scholar]
  32. Volkman A. The origin and fate of the monocyte. Ser Haematol. 1970;3(2):62–92. [PubMed] [Google Scholar]
  33. Zucker-Franklin D. The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood. J Immunol. 1974 Jan;112(1):234–240. [PubMed] [Google Scholar]
  34. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. van Furth R. Origin and kinetics of monocytes and macrophages. Semin Hematol. 1970 Apr;7(2):125–141. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES