Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Dec;32(12):3075–3077. doi: 10.1128/jcm.32.12.3075-3077.1994

Transportation of Helicobacter pylori cultures by optimal systems.

H X Xia 1, C T Keane 1, J Chen 1, J Zhang 1, E J Walsh 1, A P Moran 1, J S Hua 1, F Megraud 1, C A O'Morain 1
PMCID: PMC264234  PMID: 7883907

Abstract

Cultures of Helicobacter pylori on chocolate agar slants in bijou bottles and on chocolate agar plates inside BBL Campy Pouches were mailed from Dublin to Galway, Ireland; Bordeaux, France; and Beijing, China. Both systems maintained viability of H. pylori for at least 4 days under mailing conditions. Ninety percent of the isolates on the slants survived for 6 days, but only 30% of the isolates in the pouches survived. When the slants were stored at 4 degrees C after arrival, 50% of the isolates were recoverable 10 days after mailing. Failure of recovery was due to coccoid formation by the organisms. Contamination was not a problem in either system. Chocolate agar slants are considered the more suitable system for transporting H. pylori cultures, especially when transport time longer than 4 days is expected.

Full text

PDF
3075

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axon A. T. Helicobacter pylori infection. J Antimicrob Chemother. 1993 Jul;32 (Suppl A):61–68. doi: 10.1093/jac/32.suppl_a.61. [DOI] [PubMed] [Google Scholar]
  2. Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Humphreys H., Bourke S., Dooley C., McKenna D., Power B., Keane C. T., Sweeney E. C., O'Moráin C. Effect of treatment on Campylobacter pylori in peptic disease: a randomised prospective trial. Gut. 1988 Mar;29(3):279–283. doi: 10.1136/gut.29.3.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Megraud F., Bonnet F., Garnier M., Lamouliatte H. Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content. J Clin Microbiol. 1985 Dec;22(6):1007–1010. doi: 10.1128/jcm.22.6.1007-1010.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Owen R. J., On S. L., Costas M. Potential transport medium for Campylobacter pylori. J Clin Pathol. 1988 Dec;41(12):1337–1338. doi: 10.1136/jcp.41.12.1337-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Queiroz D. M., Mendes E. N., Rocha G. A. Indicator medium for isolation of Campylobacter pylori. J Clin Microbiol. 1987 Dec;25(12):2378–2379. doi: 10.1128/jcm.25.12.2378-2379.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Soltesz V., Zeeberg B., Wadström T. Optimal survival of Helicobacter pylori under various transport conditions. J Clin Microbiol. 1992 Jun;30(6):1453–1456. doi: 10.1128/jcm.30.6.1453-1456.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Xia H. X., English L., Keane C. T., O'Morain C. A. Enhanced cultivation of Helicobacter pylori in liquid media. J Clin Pathol. 1993 Aug;46(8):750–753. doi: 10.1136/jcp.46.8.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Xia H. X., Keane C. T., O'Morain C. A. Culture of Helicobacter pylori under aerobic conditions on solid media. Eur J Clin Microbiol Infect Dis. 1994 May;13(5):406–409. doi: 10.1007/BF01971998. [DOI] [PubMed] [Google Scholar]
  10. Xia H. X., Keane C. T., O'Morain C. A. Determination of the optimal transport system for Helicobacter pylori cultures. J Med Microbiol. 1993 Nov;39(5):334–337. doi: 10.1099/00222615-39-5-334. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES