Abstract
Investigation of the specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHEC-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent, monoclonal antibody 4E8C12, were present in numerous serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA was improved by a modified test protocol incorporating immunocapture.
Full Text
The Full Text of this article is available as a PDF (167.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borczyk A. A., Harnett N., Lombos M., Lior H. False-positive identification of Escherichia coli O157 by commercial latex agglutination tests. Lancet. 1990 Oct 13;336(8720):946–947. doi: 10.1016/0140-6736(90)92322-9. [DOI] [PubMed] [Google Scholar]
- Gannon V. P., Gyles C. L., Friendship R. W. Characteristics of verotoxigenic Escherichia coli from pigs. Can J Vet Res. 1988 Jul;52(3):331–337. [PMC free article] [PubMed] [Google Scholar]
- Griffin P. M., Tauxe R. V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98. doi: 10.1093/oxfordjournals.epirev.a036079. [DOI] [PubMed] [Google Scholar]
- Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kim M. S., Doyle M. P. Dipstick immunoassay to detect enterohemorrhagic Escherichia coli O157:H7 in retail ground beef. Appl Environ Microbiol. 1992 May;58(5):1764–1767. doi: 10.1128/aem.58.5.1764-1767.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Padhye N. V., Doyle M. P. Production and characterization of a monoclonal antibody specific for enterohemorrhagic Escherichia coli of serotypes O157:H7 and O26:H11. J Clin Microbiol. 1991 Jan;29(1):99–103. doi: 10.1128/jcm.29.1.99-103.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Padhye N. V., Doyle M. P. Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food. Appl Environ Microbiol. 1991 Sep;57(9):2693–2698. doi: 10.1128/aem.57.9.2693-2698.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas A., Chart H., Cheasty T., Smith H. R., Frost J. A., Rowe B. Vero cytotoxin-producing Escherichia coli, particularly serogroup O 157, associated with human infections in the United Kingdom: 1989-91. Epidemiol Infect. 1993 Jun;110(3):591–600. doi: 10.1017/s0950268800051013. [DOI] [PMC free article] [PubMed] [Google Scholar]