Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1978 Jun;32(3):443–450.

Class-specific antibody titres (ELISA) against the primary immunogen Helix pomatia haemocyanin (HPH) in man.

J Weits, G C de Gast, T H The, M T Esselink, A M Deelder, M Petrovic, E Mandema
PMCID: PMC1541332  PMID: 80296

Abstract

Using the indirect ELISA technique, IgM, IgG and IgA antibody titres against the primary test immunogen Helix pomatia haemocyanin (HPH) could be measured. All twenty-seven normal volunteers (age range 26-74 years) developed maximal or high IgM, IgG and IgA antibody titres 2 weeks after primary immunization with 1.0 mg HPH subcutaneously. Lower dose immunization resulted in submaximal responses. Titre kinetics in the three Ig classes correlated in height and time per person. There was no significant difference in antibody response between two adult age groups. Secondary immunization after 6 weeks, again with 1.0 mg HPH, when high titres were still present, resulted in a small titre increase in all three Ig classes. Comparison with the tanned red cell agglutination technique used previously, in combination with the 2-mercaptoethanol treatment of sera, showed the unreliability of 7S titres measured thus early in the primary immune response. Investigation of four patients with humoral immunodeficiency confirmed the class specificity of the test.

Full text

PDF
443

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Curtis J. E., Hersh E. M., Harris J. E., McBride C., Freireich E. J. The human primary immune response to keyhole limpet haemocyanin: interrelationships of delayed hypersensitivity, antibody response and in vitro blast transformation. Clin Exp Immunol. 1970 Apr;6(4):473–491. [PMC free article] [PubMed] [Google Scholar]
  2. Curtis J. E., Hersh E. M. The human secondary immune response to Keyhole limpet haemocyanin. Clin Exp Immunol. 1972 Jan;10(1):171–177. [PMC free article] [PubMed] [Google Scholar]
  3. Deelder A. M., Ruttenberg E. J., Kornelis D., Steerenberg P. A. Schistosoma mansoni: comparison of the immunoperoxidase techniques, DASS and ELISA, for human diagnosis. Exp Parasitol. 1977 Feb;41(1):133–140. doi: 10.1016/0014-4894(77)90139-4. [DOI] [PubMed] [Google Scholar]
  4. Dixon F. J., Jacot-Guillarmod H., McConahey P. J. The antibody responses of rabbits and rats to hemocyanin. J Immunol. 1966 Sep;97(3):350–355. [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. Folds J. D., Stavitsky A. B. Enhanced protein and IgM and IgG antibody synthesis following an early second injection of hemocyanin into the rabbit. J Immunol. 1971 Feb;106(2):383–391. [PubMed] [Google Scholar]
  7. Gast G. C., The T. H., Schraffordt Koops H., Snijder A. M., Huiges H. A. Primary immune response to Helix pomatia haemocyanin in malignant melanoma. Relationship between 19S and 7S antibody response and in vitro lymphocyte transformation. Clin Exp Immunol. 1976 Mar;23(3):491–498. [PMC free article] [PubMed] [Google Scholar]
  8. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  9. Konings W. N., van Driel R., van Bruggen E. F., Gruber M. Structure and properties of hemocyanins. V. Binding of oxygen and copper in Helix pomatia hemocyanin. Biochim Biophys Acta. 1969 Nov 11;194(1):55–66. doi: 10.1016/0005-2795(69)90179-2. [DOI] [PubMed] [Google Scholar]
  10. Newcomb R. W., Ishizaka K. Human diphtheria antitoxin in immunoglobulin classes IgG and IgA. J Immunol. 1967 Jul;99(1):40–48. [PubMed] [Google Scholar]
  11. Peacock D. B., Jones J. V., Gough M. The immune response to thetaX 174 in man. I. Primary and secondary antibody production in normal adults. Clin Exp Immunol. 1973 Apr;13(4):497–513. [PMC free article] [PubMed] [Google Scholar]
  12. Rowley M. J., Mackay I. R. Measurement of antibody-producing capacity in man. I. The normal response to flagellin from Salmonella adelaide. Clin Exp Immunol. 1969 Oct;5(4):407–418. [PMC free article] [PubMed] [Google Scholar]
  13. Rowley M. J., Wistar R., Mackay I. R. Measurement of antibody-producing capacity in man. V. Immunoglobulin classes of antibodies to flagellin. Immunology. 1972 Mar;22(3):475–484. [PMC free article] [PubMed] [Google Scholar]
  14. UHR J. W. THE HETEROGENEITY OF THE IMMUNE RESPONSE. Science. 1964 Jul 31;145(3631):457–464. doi: 10.1126/science.145.3631.457. [DOI] [PubMed] [Google Scholar]
  15. Weits J., de Gast G. C., The T. H., Esselink M. T., Mandema E. Cellular immunocompetence in asymptomatic paraproteinaemia. Acta Med Scand. 1977;202(1-2):17–21. doi: 10.1111/j.0954-6820.1977.tb16775.x. [DOI] [PubMed] [Google Scholar]
  16. Weits J., de Gast G. C., The T. H., Marrink J., Mandema E. Immune response in asymptomatic paraproteinemia. I. Primary and secondary antibody response to Helix pomatia hemocyanin. Scand J Immunol. 1976;5(10):1163–1171. [PubMed] [Google Scholar]
  17. de Gast G. C., The T. H., Snijder J. A. The human immune response to alpha-haemocyanin of Helix pomatia. Acta Med Scand. 1973 Oct;194(4):303–309. doi: 10.1111/j.0954-6820.1973.tb19450.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES