Abstract
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potentially be useful for demonstrating other medically important bacteria.
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- Bevanger L., Maeland J. A., Naess A. I. Agglutinins and antibodies to Francisella tularensis outer membrane antigens in the early diagnosis of disease during an outbreak of tularemia. J Clin Microbiol. 1988 Mar;26(3):433–437. doi: 10.1128/jcm.26.3.433-437.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bevanger L., Maeland J. A., Naess A. I. Competitive enzyme immunoassay for antibodies to a 43,000-molecular-weight Francisella tularensis outer membrane protein for the diagnosis of tularemia. J Clin Microbiol. 1989 May;27(5):922–926. doi: 10.1128/jcm.27.5.922-926.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown S. L., McKinney F. T., Klein G. C., Jones W. L. Evaluation of a safranin-O-stained antigen microagglutination test for francisella tularensis antibodies. J Clin Microbiol. 1980 Feb;11(2):146–148. doi: 10.1128/jcm.11.2.146-148.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlsson H. E., Lindberg A. A., Lindberg G., Hederstedt B., Karlsson K. A., Agell B. O. Enzyme-linked immunosorbent assay for immunological diagnosis of human tularemia. J Clin Microbiol. 1979 Nov;10(5):615–621. doi: 10.1128/jcm.10.5.615-621.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forsman M., Sandström G., Jaurin B. Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis. Appl Environ Microbiol. 1990 Apr;56(4):949–955. doi: 10.1128/aem.56.4.949-955.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulop M. J., Webber T., Manchee R. J., Kelly D. C. Production and characterization of monoclonal antibodies directed against the lipopolysaccharide of Francisella tularensis. J Clin Microbiol. 1991 Jul;29(7):1407–1412. doi: 10.1128/jcm.29.7.1407-1412.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geisbert T. W., Jahrling P. B., Hanes M. A., Zack P. M. Association of Ebola-related Reston virus particles and antigen with tissue lesions of monkeys imported to the United States. J Comp Pathol. 1992 Feb;106(2):137–152. doi: 10.1016/0021-9975(92)90043-t. [DOI] [PubMed] [Google Scholar]
- Geisbert T. W., Rhoderick J. B., Jahrling P. B. Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy. J Clin Pathol. 1991 Jun;44(6):521–522. doi: 10.1136/jcp.44.6.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karlsson K. A., Dahlstrand S., Hanko E., Söderlind O. Demonstration of Francisella tularensis (syn. Pasteurella tularensis) in sylvan animals with the aid of fluorescent antibodies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(5):647–651. doi: 10.1111/j.1699-0463.1970.tb04351.x. [DOI] [PubMed] [Google Scholar]
- OVERHOLT E. L., TIGERTT W. D., KADULL P. J., WARD M. K., CHARKES N. D., RENE R. M., SALZMAN T. E., STEPHENS M. An analysis of forty-two cases of laboratory-acquired tularemia. Treatment with broad spectrum antibiotics. Am J Med. 1961 May;30:785–806. doi: 10.1016/0002-9343(61)90214-5. [DOI] [PubMed] [Google Scholar]
- Syrjälä H., Koskela P., Ripatti T., Salminen A., Herva E. Agglutination and ELISA methods in the diagnosis of tularemia in different clinical forms and severities of the disease. J Infect Dis. 1986 Jan;153(1):142–145. doi: 10.1093/infdis/153.1.142. [DOI] [PubMed] [Google Scholar]